Key features and details
- HRP Mouse monoclonal [BT7R] to beta Tubulin
- Suitable for: WB
- Reacts with: Mouse, Rat, Rabbit, Chicken, Human, Non human primates
- Conjugation: HRP
- Isotype: IgG2a
Product nameHRP Anti-beta Tubulin antibody [BT7R]
See all beta Tubulin primary antibodies
DescriptionHRP Mouse monoclonal [BT7R] to beta Tubulin
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Mouse, Rat, Rabbit, Chicken, Human, Non human primates
Synthetic peptide corresponding to Human beta Tubulin (N terminal) conjugated to Keyhole Limpet Haemocyanin (KLH).
Database link: P07437
- A431, U2OS, 293T, Jurkat, HeLa, COS7, C2C12 and NRK cell lysates.
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C.
Concentration information loading...
- TMB ELISA Substrate (Highest Sensitivity) (ab171522)
- TMB ELISA Substrate (High Sensitivity) (ab171523)
- TMB ELISA Substrate (Fast Kinetic Rate) (ab171524)
- TMB ELISA Substrate (Slow Kinetic Rate) (ab171525)
- TMB ELISA Substrate (Slower Kinetic Rate) (ab171526)
- TMB ELISA Substrate (Slowest Kinetic Rate) (ab171527)
- 450 nm Stop Solution for TMB Substrate (ab171529)
- 650 nm Stop Solution for TMB Substrate (ab171531)
- Immunoassay Blocking Buffer (ab171534)
- Immunoassay Blocking (BSA Free) (ab171535)
Our Abpromise guarantee covers the use of ab173840 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/2000. Predicted molecular weight: 50 kDa.|
FunctionTubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.
Tissue specificityUbiquitously expressed with highest levels in spleen, thymus and immature brain.
Involvement in diseaseCortical dysplasia, complex, with other brain malformations 6
Skin creases, congenital symmetric circumferential, 1
Sequence similaritiesBelongs to the tubulin family.
DomainThe highly acidic C-terminal region may bind cations such as calcium.
modificationsSome glutamate residues at the C-terminus are polyglutamylated, resulting in polyglutamate chains on the gamma-carboxyl group (PubMed:26875866). Polyglutamylation plays a key role in microtubule severing by spastin (SPAST). SPAST preferentially recognizes and acts on microtubules decorated with short polyglutamate tails: severing activity by SPAST increases as the number of glutamates per tubulin rises from one to eight, but decreases beyond this glutamylation threshold (PubMed:26875866).
Some glutamate residues at the C-terminus are monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella). Both polyglutamylation and monoglycylation can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of monoglycylation is still unclear.
Phosphorylated on Ser-172 by CDK1 during the cell cycle, from metaphase to telophase, but not in interphase. This phosphorylation inhibits tubulin incorporation into microtubules.
Cellular localizationCytoplasm, cytoskeleton.
- Information by UniProt
- Beta 4 tubulin antibody
- Beta 5 tubulin antibody
- beta Ib tubulin antibody
All lanes : HRP Anti-beta Tubulin antibody [BT7R] (ab173840) at 1/1000 dilution
Lane 1 : A431 cell lysate
Lane 2 : U2OS cell lysate
Lane 3 : 293T cell lysate
Lane 4 : Jurkat cell lysate
Lane 5 : HeLa cell lysate
Lane 6 : COS7 cell lysate
Lane 7 : C2C12 cell lysate
Lane 8 : NRK cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 50 kDa
4-20% Tris-HCl polyacrylamide gel.
ab173840 has been referenced in 1 publication.
- Chen C et al. Proteomic analysis of the effects of CSF-1 and IL-1a on dental follicle cells. Mol Med Rep 14:2405-14 (2016). PubMed: 27484316