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  1. Link

    hrp-beta-tubulin-antibody-bt7r-ab173840.pdf

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Isotype/Loading Controls Loading Controls Tubulin
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HRP Anti-beta Tubulin antibody [BT7R] (ab173840)

  • Datasheet
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Western blot - HRP Anti-beta Tubulin antibody [BT7R] (ab173840)

    Key features and details

    • HRP Mouse monoclonal [BT7R] to beta Tubulin
    • Suitable for: WB
    • Reacts with: Mouse, Rat, Rabbit, Chicken, Human, Non human primates
    • Conjugation: HRP
    • Isotype: IgG2a

    You may also be interested in

    Protein
    Product image
    Recombinant Human beta Tubulin protein (ab70187)

    View more associated products

    Overview

    • Product name

      HRP Anti-beta Tubulin antibody [BT7R]
      See all beta Tubulin primary antibodies
    • Description

      HRP Mouse monoclonal [BT7R] to beta Tubulin
    • Host species

      Mouse
    • Conjugation

      HRP
    • Tested applications

      Suitable for: WBmore details
    • Species reactivity

      Reacts with: Mouse, Rat, Rabbit, Chicken, Human, Non human primates
    • Immunogen

      Synthetic peptide corresponding to Human beta Tubulin (N terminal) conjugated to Keyhole Limpet Haemocyanin (KLH).
      Database link: P07437

    • Positive control

      • A431, U2OS, 293T, Jurkat, HeLa, COS7, C2C12 and NRK cell lysates.
    • General notes

      Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

      Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

      We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

      In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

      We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

      Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

      Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Store at +4°C.
    • Concentration information loading...
    • Clonality

      Monoclonal
    • Clone number

      BT7R
    • Isotype

      IgG2a
    • Research areas

      • Isotype/Loading Controls
      • Loading Controls
      • Tubulin
      • Tags & Cell Markers
      • Subcellular Markers
      • Cytoskeleton
      • Microtubules
      • Signal Transduction
      • Cytoskeleton / ECM
      • Cytoskeleton
      • Microtubules
      • Tubulin
      • Isotype/Loading Controls
      • Loading Controls
      • Beta Tubulin

    Associated products

    • Alternative Versions

      • Biotin Anti-beta Tubulin antibody [BT7R] (ab173839)
    • Positive Controls

      • HeLa whole cell lysate (ab150035)
      • HeLa whole cell lysate (ab29545)
      • Jurkat whole cell lysate (ab30128)
      • A431 whole cell lysate (ab30132)
      • Jurkat whole cell lysate (ab7899)
      • A431 whole cell lysate (ab7909)
    • Recombinant Protein

      • Recombinant Human beta Tubulin protein (ab70187)
    • Related Products

      • TMB ELISA Substrate (Highest Sensitivity) (ab171522)
      • TMB ELISA Substrate (High Sensitivity) (ab171523)
      • TMB ELISA Substrate (Fast Kinetic Rate) (ab171524)
      • TMB ELISA Substrate (Slow Kinetic Rate) (ab171525)
      • TMB ELISA Substrate (Slower Kinetic Rate) (ab171526)
      • TMB ELISA Substrate (Slowest Kinetic Rate) (ab171527)
      • 450 nm Stop Solution for TMB Substrate (ab171529)
      • 650 nm Stop Solution for TMB Substrate (ab171531)
      • Immunoassay Blocking Buffer (ab171534)
      • Immunoassay Blocking (BSA Free) (ab171535)

    Applications

    Our Abpromise guarantee covers the use of ab173840 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    WB 1/500 - 1/2000. Predicted molecular weight: 50 kDa.

    Target

    • Function

      Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.
    • Tissue specificity

      Ubiquitously expressed with highest levels in spleen, thymus and immature brain.
    • Involvement in disease

      Cortical dysplasia, complex, with other brain malformations 6
      Skin creases, congenital symmetric circumferential, 1
    • Sequence similarities

      Belongs to the tubulin family.
    • Domain

      The highly acidic C-terminal region may bind cations such as calcium.
    • Post-translational
      modifications

      Some glutamate residues at the C-terminus are polyglutamylated, resulting in polyglutamate chains on the gamma-carboxyl group (PubMed:26875866). Polyglutamylation plays a key role in microtubule severing by spastin (SPAST). SPAST preferentially recognizes and acts on microtubules decorated with short polyglutamate tails: severing activity by SPAST increases as the number of glutamates per tubulin rises from one to eight, but decreases beyond this glutamylation threshold (PubMed:26875866).
      Some glutamate residues at the C-terminus are monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella). Both polyglutamylation and monoglycylation can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of monoglycylation is still unclear.
      Phosphorylated on Ser-172 by CDK1 during the cell cycle, from metaphase to telophase, but not in interphase. This phosphorylation inhibits tubulin incorporation into microtubules.
    • Cellular localization

      Cytoplasm, cytoskeleton.
    • Target information above from: UniProt accession P07437 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 396254 Chicken
      • Entrez Gene: 203068 Human
      • Entrez Gene: 22154 Mouse
      • Entrez Gene: 29214 Rat
      • Omim: 191130 Human
      • SwissProt: P07437 Human
      • SwissProt: P99024 Mouse
      • SwissProt: P69897 Rat
      • Unigene: 636480 Human
      see all
    • Alternative names

      • Beta 4 tubulin antibody
      • Beta 5 tubulin antibody
      • beta Ib tubulin antibody
      • Beta1 tubulin antibody
      • Class I beta tubulin antibody
      • M40 antibody
      • MGC117247 antibody
      • MGC16435 antibody
      • OK/SW cl.56 antibody
      • OK/SWcl.56 antibody
      • TBB5_HUMAN antibody
      • TUBB 1 antibody
      • TUBB 2 antibody
      • TUBB 5 antibody
      • TUBB antibody
      • TUBB1 antibody
      • TUBB2 antibody
      • TUBB5 antibody
      • tubulin beta 1 chain antibody
      • Tubulin beta 2 chain antibody
      • tubulin beta 5 chain antibody
      • Tubulin beta chain antibody
      • Tubulin beta class I antibody
      • tubulin beta polypeptide antibody
      • Tubulin beta-5 chain antibody
      see all

    Images

    • Western blot - HRP Anti-beta Tubulin antibody [BT7R] (ab173840)
      Western blot - HRP Anti-beta Tubulin antibody [BT7R] (ab173840)
      All lanes : HRP Anti-beta Tubulin antibody [BT7R] (ab173840) at 1/1000 dilution

      Lane 1 : A431 cell lysate
      Lane 2 : U2OS cell lysate
      Lane 3 : 293T cell lysate
      Lane 4 : Jurkat cell lysate
      Lane 5 : HeLa cell lysate
      Lane 6 : COS7 cell lysate
      Lane 7 : C2C12 cell lysate
      Lane 8 : NRK cell lysate

      Lysates/proteins at 50 µg per lane.

      Developed using the ECL technique.

      Predicted band size: 50 kDa



      4-20% Tris-HCl polyacrylamide gel.

    Protocols

    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
  • References (1)

    Publishing research using ab173840? Please let us know so that we can cite the reference in this datasheet.

    ab173840 has been referenced in 1 publication.

    • Chen C  et al. Proteomic analysis of the effects of CSF-1 and IL-1a on dental follicle cells. Mol Med Rep 14:2405-14 (2016). PubMed: 27484316

    Customer reviews and Q&As

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