Application
Western blot
Sample
Mouse Cell lysate - whole cell (Primary macrophage)
Gel Running Conditions
Reduced Denaturing (4-12%)
Loading amount
10 µg
Specification
Primary macrophage
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
Other product details
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 22°C
Dilution
1/10000
Secondary antibody
Secondary antibody
None used
Detection
Detection method
ECL
Exposure
5 second(s)
Bands
Specific: 55 kDa
Additional data
Additional Notes
Molecular weight was determined using the PageRuler prestained protein ladder.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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Submitted Oct 06 2017