Application
Western blot
Sample
Mouse Tissue lysate - whole (pancreas)
Gel Running Conditions
Reduced Denaturing
Loading amount
5 µg
Specification
pancreas
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 20°C
Other product details
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 20°C · Diluent: 1% non fat milk
Dilution
1/10000
Secondary antibody
Secondary antibody
None used
Detection
Detection method
ECL
Exposure
5 minute(s) and 0 second(s)
Bands
Specific: 55 kDa
Additional data
Additional Notes
works great with most of the mouse tissue. easy to use. strong and very specific signals.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
Submitted Jan 21 2022