HRP Anti-Biotin antibody (ab53468)

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Key features and details

  • HRP Rabbit polyclonal to Biotin
  • Suitable for: WB, Competitive ELISA
  • Reacts with: Species independent
  • Conjugation: HRP
  • Isotype: IgG

Overview

  • Product name

    HRP Anti-Biotin antibody
    See all Biotin primary antibodies

  • Description

    HRP Rabbit polyclonal to Biotin

  • Host species

    Rabbit

  • Conjugation

    HRP

  • Specificity

    Reacts with free biotin and biotinylated antibody and proteins.

  • Tested applications

    Suitable for: WB, Competitive ELISAmore details

  • Species reactivity

    Reacts with: Species independent

  • Immunogen

    Chemical/ Small Molecule corresponding to Biotin conjugated to keyhole limpet haemocyanin.

  • General notes

    Reductive amination; Ab/HRP ratio=1/10.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Applications

Our Abpromise guarantee covers the use of ab53468 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.25 - 1 µg/ml.
Competitive ELISA Use a concentration of 0.25 - 1 µg/ml.

Target

  • Relevance

    Biotin is a water soluble vitamin, generally classified as a B complex vitamin, also called vitamin B4. After the initial discovery of biotin, nearly forty years of research were required to establish it as a vitamin. Biotin is required by all organisms but can only be synthesized by bacteria, yeasts, molds, algae, and some plant species. Biotin is required as prosthetic group of enzymes involved in incorporation of carbon dioxide into organic compounds. Biotin has a MW of 244 Da.

  • Alternative names

    • Vitamin B4 antibody

References (1)

Publishing research using ab53468? Please let us know so that we can cite the reference in this datasheet.

ab53468 has been referenced in 1 publication.

  • Króliczewski J  et al. ALB3 Insertase Mediates Cytochrome b6 Co-translational Import into the Thylakoid Membrane. Sci Rep 6:34557 (2016). WB . PubMed: 27698412

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