HRP Anti-BNP antibody [24C5] (ab14699)

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Key features and details

  • HRP Mouse monoclonal [24C5] to BNP
  • Reacts with: Human
  • Conjugation: HRP
  • Isotype: IgG1

Overview

  • Product name

    HRP Anti-BNP antibody [24C5]
    See all BNP primary antibodies

  • Description

    HRP Mouse monoclonal [24C5] to BNP

  • Host species

    Mouse

  • Conjugation

    HRP

  • Specificity

    MAb 1B6 recognizes proBNP in Western Blotting after tricine-SDS-gel electrophoresis in reducing conditions.

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide:

    FGRKMDRISSSS

    , corresponding to N terminal amino acids 11-22 of Human BNP
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • General notes

    Immunoglobulin concentrations vary from batch to batch (generally 5-8 mg/ml for this clone). Please contact technical services for the latest information.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

Applications

  • Application notes
    ELISA: Use at an assay dependent dilution.
    sELISA: Use at an assay dependent dilution.
    Can be paired for ELISA with Mouse monoclonal [50E1] to BNP (ab20984).

    WB: Use at an assay dependent dilution. Predicted molecular weight: 16 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • Target

    • Relevance

      Brain natriuretic peptide (BNP) circulates in blood as a peptide hormone with natriuretic, vasodilatory and renin inhibitory properties. BNP is secreted predominantly by the left ventricular myocytes in response to volume expansion and pressure overload. BNP belongs to a family of structurally similar peptide hormones, which includes atrial natriuretic peptide (ANP), BNP, C type natriuretic peptide (CNP) and urodilatin. These peptides are characterized by a common 17 amino acid ring structure with a disulfide bond between two cystein residues. This ring structure shows high homology between different natriuretic peptides (eleven of the 17 amino acid residues are homologous in the ring of each of the natriuretic peptides). BNP is a 32 amino acid peptide with disulfide bond between the cysteine residues Cys10 and Cys26. In earlier studies it has been demonstrated that BNP concentration in blood increases with the severity of congestive heart failure. Quantitative measurement of BNP in blood provides an objective indicator of congestive heart failure severity.

    • Cellular localization

      Secreted

    • Database links

      • Alternative names

        • BNP(1-28) antibody
        • BNP(1-29) antibody
        • BNP(1-30) antibody
        • BNP(1-32) antibody
        • BNP(2-31) antibody
        • BNP(3-29) antibody
        • BNP(3-30) antibody
        • BNP(3-32) antibody
        • BNP(4-27) antibody
        • BNP(4-29) antibody
        • BNP(4-30) antibody
        • BNP(4-31) antibody
        • BNP(4-32) antibody
        • BNP(5-29) antibody
        • BNP(5-31) antibody
        • BNP(5-32) antibody
        • Brain natriuretic peptide 32 antibody
        • Brain type natriuretic peptide antibody
        • Gamma brain natriuretic peptide antibody
        • Natriuretic peptide B antibody
        • Natriuretic peptide precursor B antibody
        • natriuretic peptide, brain type antibody
        • Natriuretic peptides B antibody
        • natriuretic protein antibody
        • NPPB antibody
        see all

      References (2)

      Publishing research using ab14699? Please let us know so that we can cite the reference in this datasheet.

      ab14699 has been referenced in 2 publications.

      • Gong Y  et al. Improved LFIAs for highly sensitive detection of BNP at point-of-care. Int J Nanomedicine 12:4455-4466 (2017). PubMed: 28670119
      • Ding YD  et al. A sandwich ELISA for assessment of pharmacokinetics of HSA-(BNP)2 fusion protein in mouse plasma. J Pharm Biomed Anal 51:658-63 (2010). ELISA . PubMed: 19836183

      Customer reviews and Q&As

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      1-2 of 2 Abreviews or Q&A

      Question

      What is the concentration?

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      Answer

      Thank you for your email. For ab13111, the concentration of the batch that is currently in stock is 4.7 mg/ml. For ab14699, the concentration of the batch that is currently in stock is 5.80 mg/ml. If you have any additional questions, please contact us again.

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      Question

      I am a gradutate student at Boston University School of Medicine. I am interested in your anti-BNP and anti-proBNP monoclonal antibodies. Is there any information on whether this detects mouse BNP? Additionally, is it possible to purchase any of these antibodies in sizes smaller than 200 ug? I cannot convince my advisor to buy that much of an antibody that we have not tested before.

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      Answer

      Thank you for your enquiry. Thank you for your enquiry. All the information we have on species cross reactivity is specified on the datasheet, these are updated as soon as any new information is brought to our attention. As far as we are aware, cross reactivity with mouse BNP has not yet been tested for use with these antibodies. Should you decide to go ahead and purchase this product, please let us know how you get on and in return we will forward a reward of your choice, typically an Amazon gift voucher. Please let me know if you require any further assistance.

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