HRP Anti-BNP antibody [24C5] (ab14699)
Key features and details
- HRP Mouse monoclonal [24C5] to BNP
- Reacts with: Human
- Conjugation: HRP
- Isotype: IgG1
Overview
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Product name
HRP Anti-BNP antibody [24C5]
See all BNP primary antibodies -
Description
HRP Mouse monoclonal [24C5] to BNP -
Host species
Mouse -
Conjugation
HRP -
Specificity
MAb 1B6 recognizes proBNP in Western Blotting after tricine-SDS-gel electrophoresis in reducing conditions. -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide:
FGRKMDRISSSS
, corresponding to N terminal amino acids 11-22 of Human BNP -
General notes
Immunoglobulin concentrations vary from batch to batch (generally 5-8 mg/ml for this clone). Please contact technical services for the latest information.Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.40
Preservative: 0.05% Proclin 300
Constituents: 0.8% Sodium chloride, 0.02% Potassium chloride, 0.024% Monobasic dihydrogen potassium phosphate, 0.18% Dibasic monohydrogen sodium phosphate -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
24C5 -
Myeloma
Sp2/0 -
Isotype
IgG1 -
Research areas
Associated products
-
Positive Controls
-
Recombinant Protein
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-
sELISA pair antibody
Applications
sELISA: Use at an assay dependent dilution.
Can be paired for ELISA with Mouse monoclonal [50E1] to BNP (ab20984).
WB: Use at an assay dependent dilution. Predicted molecular weight: 16 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
-
Relevance
Brain natriuretic peptide (BNP) circulates in blood as a peptide hormone with natriuretic, vasodilatory and renin inhibitory properties. BNP is secreted predominantly by the left ventricular myocytes in response to volume expansion and pressure overload. BNP belongs to a family of structurally similar peptide hormones, which includes atrial natriuretic peptide (ANP), BNP, C type natriuretic peptide (CNP) and urodilatin. These peptides are characterized by a common 17 amino acid ring structure with a disulfide bond between two cystein residues. This ring structure shows high homology between different natriuretic peptides (eleven of the 17 amino acid residues are homologous in the ring of each of the natriuretic peptides). BNP is a 32 amino acid peptide with disulfide bond between the cysteine residues Cys10 and Cys26. In earlier studies it has been demonstrated that BNP concentration in blood increases with the severity of congestive heart failure. Quantitative measurement of BNP in blood provides an objective indicator of congestive heart failure severity. -
Cellular localization
Secreted -
Database links
- Entrez Gene: 4879 Human
- Omim: 600295 Human
- SwissProt: P16860 Human
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Alternative names
- BNP(1-28) antibody
- BNP(1-29) antibody
- BNP(1-30) antibody
see all
References (2)
ab14699 has been referenced in 2 publications.
- Gong Y et al. Improved LFIAs for highly sensitive detection of BNP at point-of-care. Int J Nanomedicine 12:4455-4466 (2017). PubMed: 28670119
- Ding YD et al. A sandwich ELISA for assessment of pharmacokinetics of HSA-(BNP)2 fusion protein in mouse plasma. J Pharm Biomed Anal 51:658-63 (2010). ELISA . PubMed: 19836183
