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    hrp-bnp-antibody-24c5-ab14699.pdf

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Signal Transduction Growth Factors/Hormones Hormones
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HRP Anti-BNP antibody [24C5] (ab14699)

  • Datasheet
  • SDS
Submit a review Q&A (2)References (2)

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Key features and details

  • HRP Mouse monoclonal [24C5] to BNP
  • Reacts with: Human
  • Conjugation: HRP
  • Isotype: IgG1

You may also be interested in

Protein
Recombinant Human BNP protein (ab151881)
Primary
Anti-BNP antibody [50E1] (ab20984)
Primary
Product image
Anti-BNP antibody [EPR22339-65] (ab243440)

View more associated products

Overview

  • Product name

    HRP Anti-BNP antibody [24C5]
    See all BNP primary antibodies
  • Description

    HRP Mouse monoclonal [24C5] to BNP
  • Host species

    Mouse
  • Conjugation

    HRP
  • Specificity

    MAb 1B6 recognizes proBNP in Western Blotting after tricine-SDS-gel electrophoresis in reducing conditions.
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide:

    FGRKMDRISSSS

    , corresponding to N terminal amino acids 11-22 of Human BNP
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • General notes

    Immunoglobulin concentrations vary from batch to batch (generally 5-8 mg/ml for this clone). Please contact technical services for the latest information.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C.
  • Storage buffer

    pH: 7.40
    Preservative: 0.05% Proclin 300
    Constituents: 0.8% Sodium chloride, 0.02% Potassium chloride, 0.024% Monobasic dihydrogen potassium phosphate, 0.18% Dibasic monohydrogen sodium phosphate
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    24C5
  • Myeloma

    Sp2/0
  • Isotype

    IgG1
  • Research areas

    • Signal Transduction
    • Growth Factors/Hormones
    • Hormones
    • Cardiovascular
    • Blood
    • Blood Pressure regulation
    • Cardiovascular
    • Heart
    • Hypertrophy
    • Hormones
    • Cardiovascular
    • Heart
    • Hypertrophy
    • Other

Associated products

  • Positive Controls

    • Recombinant Human BNP protein (ab87200)
  • Recombinant Protein

    • Recombinant Human BNP protein (ab87200)
  • Related Products

    • TMB ELISA Substrate (Highest Sensitivity) (ab171522)
    • TMB ELISA Substrate (High Sensitivity) (ab171523)
    • TMB ELISA Substrate (Fast Kinetic Rate) (ab171524)
    • TMB ELISA Substrate (Slow Kinetic Rate) (ab171525)
    • TMB ELISA Substrate (Slower Kinetic Rate) (ab171526)
    • TMB ELISA Substrate (Slowest Kinetic Rate) (ab171527)
    • 450 nm Stop Solution for TMB Substrate (ab171529)
    • 650 nm Stop Solution for TMB Substrate (ab171531)
    • Immunoassay Blocking Buffer (ab171534)
    • Immunoassay Blocking (BSA Free) (ab171535)
  • sELISA pair antibody

    • Anti-BNP antibody [50E1] (ab20984)

Applications

  • Application notes
    ELISA: Use at an assay dependent dilution.
    sELISA: Use at an assay dependent dilution.
    Can be paired for ELISA with Mouse monoclonal [50E1] to BNP (ab20984).

    WB: Use at an assay dependent dilution. Predicted molecular weight: 16 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Relevance

      Brain natriuretic peptide (BNP) circulates in blood as a peptide hormone with natriuretic, vasodilatory and renin inhibitory properties. BNP is secreted predominantly by the left ventricular myocytes in response to volume expansion and pressure overload. BNP belongs to a family of structurally similar peptide hormones, which includes atrial natriuretic peptide (ANP), BNP, C type natriuretic peptide (CNP) and urodilatin. These peptides are characterized by a common 17 amino acid ring structure with a disulfide bond between two cystein residues. This ring structure shows high homology between different natriuretic peptides (eleven of the 17 amino acid residues are homologous in the ring of each of the natriuretic peptides). BNP is a 32 amino acid peptide with disulfide bond between the cysteine residues Cys10 and Cys26. In earlier studies it has been demonstrated that BNP concentration in blood increases with the severity of congestive heart failure. Quantitative measurement of BNP in blood provides an objective indicator of congestive heart failure severity.
    • Cellular localization

      Secreted
    • Database links

      • Entrez Gene: 4879 Human
      • Omim: 600295 Human
      • SwissProt: P16860 Human
      • Alternative names

        • BNP(1-28) antibody
        • BNP(1-29) antibody
        • BNP(1-30) antibody
        • BNP(1-32) antibody
        • BNP(2-31) antibody
        • BNP(3-29) antibody
        • BNP(3-30) antibody
        • BNP(3-32) antibody
        • BNP(4-27) antibody
        • BNP(4-29) antibody
        • BNP(4-30) antibody
        • BNP(4-31) antibody
        • BNP(4-32) antibody
        • BNP(5-29) antibody
        • BNP(5-31) antibody
        • BNP(5-32) antibody
        • Brain natriuretic peptide 32 antibody
        • Brain type natriuretic peptide antibody
        • Gamma brain natriuretic peptide antibody
        • Natriuretic peptide B antibody
        • Natriuretic peptide precursor B antibody
        • natriuretic peptide, brain type antibody
        • Natriuretic peptides B antibody
        • natriuretic protein antibody
        • NPPB antibody
        see all

      Protocols

      • Western blot protocols

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet
      • SDS
    • References (2)

      Publishing research using ab14699? Please let us know so that we can cite the reference in this datasheet.

      ab14699 has been referenced in 2 publications.

      • Gong Y  et al. Improved LFIAs for highly sensitive detection of BNP at point-of-care. Int J Nanomedicine 12:4455-4466 (2017). PubMed: 28670119
      • Ding YD  et al. A sandwich ELISA for assessment of pharmacokinetics of HSA-(BNP)2 fusion protein in mouse plasma. J Pharm Biomed Anal 51:658-63 (2010). ELISA . PubMed: 19836183

      Customer reviews and Q&As

      Show All Reviews Q&A
      Submit a review Submit a question

      1-2 of 2 Abreviews or Q&A

      Question

      What is the concentration?

      Read More

      Abcam community

      Verified customer

      Asked on Aug 01 2005

      Answer

      Thank you for your email. For ab13111, the concentration of the batch that is currently in stock is 4.7 mg/ml. For ab14699, the concentration of the batch that is currently in stock is 5.80 mg/ml. If you have any additional questions, please contact us again.

      Read More

      Abcam Scientific Support

      Answered on Aug 02 2005

      Question

      I am a gradutate student at Boston University School of Medicine. I am interested in your anti-BNP and anti-proBNP monoclonal antibodies. Is there any information on whether this detects mouse BNP? Additionally, is it possible to purchase any of these antibodies in sizes smaller than 200 ug? I cannot convince my advisor to buy that much of an antibody that we have not tested before.

      Read More

      Abcam community

      Verified customer

      Asked on Oct 11 2004

      Answer

      Thank you for your enquiry. Thank you for your enquiry. All the information we have on species cross reactivity is specified on the datasheet, these are updated as soon as any new information is brought to our attention. As far as we are aware, cross reactivity with mouse BNP has not yet been tested for use with these antibodies. Should you decide to go ahead and purchase this product, please let us know how you get on and in return we will forward a reward of your choice, typically an Amazon gift voucher. Please let me know if you require any further assistance.

      Read More

      Abcam Scientific Support

      Answered on Oct 15 2004

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com

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