HRP Anti-Bovine Serum Albumin antibody (ab7637)

Reviews (3)Q&A (1)References (1)

Key features and details

  • HRP Rabbit polyclonal to Bovine Serum Albumin
  • Suitable for: ELISA, IHC-Fr, Dot blot, Immunomicroscopy, WB
  • Reacts with: Cow
  • Conjugation: HRP
  • Isotype: IgG

Overview

  • Product name

    HRP Anti-Bovine Serum Albumin antibody
    See all Bovine Serum Albumin primary antibodies

  • Description

    HRP Rabbit polyclonal to Bovine Serum Albumin

  • Host species

    Rabbit

  • Conjugation

    HRP

  • Tested applications

    Suitable for: ELISA, IHC-Fr, Dot blot, Immunomicroscopy, WBmore details

  • Species reactivity

    Reacts with: Cow

  • Immunogen

    Full length native Albumin (purified).

  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid

  • Storage instructions

    Store at +4°C. Store In the Dark.

  • Storage buffer

    Preservative: 0.01% Gentamicin sulphate
    Constituents: 1% Polyethylene glycol, 0.42% Potassium phosphate, 0.87% Sodium chloride
  • Concentration information loading...

  • Purity

    IgG fraction

  • Purification notes

    This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis.

  • Clonality

    Polyclonal

  • Isotype

    IgG

  • Research areas

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab7637 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA
Use at an assay dependent concentration.
IHC-Fr
Use at an assay dependent concentration.
Dot blot
Use at an assay dependent concentration.
Immunomicroscopy
Use at an assay dependent concentration.
WB (3)
Use at an assay dependent concentration.
Notes
ELISA
Use at an assay dependent concentration.
IHC-Fr
Use at an assay dependent concentration.
Dot blot
Use at an assay dependent concentration.
Immunomicroscopy
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration.

Target

  • Information by UniProt

  • Database links

    • Alternative names

      • alb antibody
      • ALBU_HUMAN antibody
      • Albumin (32 AA) antibody
      • Albumin (AA 34) antibody
      • Albumin antibody
      • BSA antibody
      • Cell growth inhibiting protein 42 antibody
      • Growth inhibiting protein 20 antibody
      • PRO0883 antibody
      • PRO0903 antibody
      • PRO1341 antibody
      • Serum albumin antibody
      see all

    References (1)

    Publishing research using ab7637? Please let us know so that we can cite the reference in this datasheet.

    ab7637 has been referenced in 1 publication.

    • Ludwig AK  et al. Precipitation with polyethylene glycol followed by washing and pelleting by ultracentrifugation enriches extracellular vesicles from tissue culture supernatants in small and large scales. J Extracell Vesicles 7:1528109 (2018). WB . PubMed: 30357008

    Customer reviews and Q&As

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    1-4 of 4 Abreviews or Q&A

    Western blot abreview for Anti-Bovine Serum Albumin antibody (HRP)

     Excellent
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Cow Tissue lysate - whole (Liver)
    Gel Running Conditions
    Reduced Denaturing (gel 12%)
    Loading amount
    5 µg
    Specification
    Liver
    Blocking step
    Milk as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 3% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    Submitted Mar 07 2018

    Western blot abreview for Anti-Bovine Serum Albumin antibody (HRP)

     Average
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    African Green Monkey Cell lysate - whole cell (kidney epithelial and kidney fibroblast)
    Loading amount
    20 µg
    Specification
    kidney epithelial and kidney fibroblast
    Gel Running Conditions
    Reduced Denaturing (10% SDS)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    Submitted Apr 29 2010

    Western blot abreview for Anti-Bovine Serum Albumin antibody (HRP)

     Good
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (Cell Types: lymphoblast, monocytes, epithelial)
    Loading amount
    20 µg
    Specification
    Cell Types: lymphoblast, monocytes, epithelial
    Gel Running Conditions
    Reduced Denaturing
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    Submitted Apr 28 2010

    Question

    sandwich ELISA with ab3781 and ab7637
    using BSA
    problem: no signal
    sandwich ELISA is untested for both antibodies

    send testing discount code for trying in sELISA

    Read More
    Answer

    Thank you for your phone call.
    I am very sorry to hear that these antibodies did not work together in sandwich ELISA. I am sending you the following discount codes as compensation for trying the antibodies in an untested application. Unfortunately, our guarantee does not apply in this case.
    Please let me know the other 2 anti-BSA antibodies that you would like to try together in sandwich ELISA and I'd be happy to issue you the testing discount codes as well.
    DISCOUNT CODE: xxx for ab3781
    DISCOUNT CODE: xxx for ab7637
    Expiration date: xxx
    I am very pleased to hear you would like to accept our offer and test ab3781 and ab7637 in sandwich ELISA. Each code will give you 1 free PRIMARY ANTIBODY before the expiration date. To redeem this offer, please submit an Abreview for sandwich ELISA and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.
    Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.
    Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.
    The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

    Read More

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