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    hrp-brd4-antibody-epr51502-ab197609.pdf

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Epigenetics and Nuclear Signaling Chromatin Binding Proteins Acetyl Lysine
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RecombinantRabMAb

Recombinant HRP Anti-Brd4 antibody [EPR5150(2)] (ab197609)

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  • Certificate of Compliance
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Western blot - HRP Anti-Brd4 antibody [EPR5150(2)] (ab197609)
  • HRP Anti-Brd4 antibody [EPR5150(2)] (ab197609)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • HRP Rabbit monoclonal [EPR5150(2)] to Brd4
  • Suitable for: WB
  • Reacts with: Mouse, Human
  • Conjugation: HRP

You may also be interested in

Protein
Product image
Recombinant Human Brd4 protein (Tagged) (ab176941)

View more associated products

Overview

  • Product name

    HRP Anti-Brd4 antibody [EPR5150(2)]
    See all Brd4 primary antibodies
  • Description

    HRP Rabbit monoclonal [EPR5150(2)] to Brd4
  • Host species

    Rabbit
  • Conjugation

    HRP
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat
  • Immunogen

    Synthetic peptide within Human Brd4 aa 150-250. The exact sequence is proprietary.

  • Positive control

    • WB: HeLa, CaCo2, RAW264.7 and NIH3T3 whole cell lysates.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark.
  • Dissociation constant (KD)

    KD = 3.20 x 10 -11 M
    Learn more about KD
  • Storage buffer

    pH: 7.40
    Preservative: 0.1% 10% Proclin 300 Solution
    Constituents: 30% Glycerol, 1% BSA, PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Monoclonal
  • Clone number

    EPR5150(2)
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • Chromatin Binding Proteins
    • Acetyl Lysine
    • Cancer
    • Oncoproteins/suppressors
    • Tumor suppressors
    • Other
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Bromodomain containing

Associated products

  • Alternative Versions

    • Anti-Brd4 antibody [EPR5150(2)] (ab128874)
    • Anti-Brd4 antibody [EPR5150(2)] - BSA and Azide free (ab182446)
    • Alexa Fluor® 488 Anti-Brd4 antibody [EPR5150(2)] (ab197606)
    • Alexa Fluor® 647 Anti-Brd4 antibody [EPR5150(2)] (ab197608)
    • Alexa Fluor® 594 Anti-Brd4 antibody [EPR5150(2)] (ab207057)
  • Isotype control

    • HRP Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab199507)
  • Positive Controls

    • HeLa nuclear extract lysate (ab14655)
    • NIH 3T3 nuclear extract lysate (ab14874)
    • HeLa whole cell lysate (ab150035)
  • Recombinant Protein

    • Recombinant Human Brd4 protein (Tagged) (ab176941)
  • Related Products

    • ECL Substrate Kit (High Sensitivity) (ab133406)

Applications

Our Abpromise guarantee covers the use of ab197609 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/5000. Detects a band of approximately 152 kDa (predicted molecular weight: 152 kDa).

Target

  • Function

    Plays a role in a process governing chromosomal dynamics during mitosis.
  • Tissue specificity

    Ubiquitously expressed.
  • Involvement in disease

    Note=A chromosomal aberration involving BRD4 is found in a rare, aggressive, and lethal carcinoma arising in midline organs of young people. Translocation t(15;19)(q14;p13) with NUT which produces a BRD4-NUT fusion protein.
  • Sequence similarities

    Contains 2 bromo domains.
  • Cellular localization

    Nucleus.
  • Target information above from: UniProt accession O60885 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 23476 Human
    • Entrez Gene: 57261 Mouse
    • Entrez Gene: 362844 Rat
    • Omim: 608749 Human
    • SwissProt: O60885 Human
    • SwissProt: Q9ESU6 Mouse
    • Unigene: 187763 Human
    • Unigene: 253518 Mouse
    • Alternative names

      • Brd4 antibody
      • BRD4-NUT FUSION antibody
      • BRD4-NUT fusion oncoprotein antibody
      • BRD4_HUMAN antibody
      • Bromodomain containing 4 antibody
      • bromodomain containing protein 4 antibody
      • Bromodomain-containing protein 4 antibody
      • CAP antibody
      • chromosome associated protein antibody
      • HUNK1 antibody
      • HUNKI antibody
      • MCAP antibody
      • Mitotic chromosome-associated protein antibody
      • Protein HUNK1 antibody
      see all

    Images

    • Western blot - HRP Anti-Brd4 antibody [EPR5150(2)] (ab197609)
      Western blot - HRP Anti-Brd4 antibody [EPR5150(2)] (ab197609)
      All lanes : HRP Anti-Brd4 antibody [EPR5150(2)] (ab197609) at 1/5000 dilution

      Lane 1 : HeLa whole cell lysate (ab150035)
      Lane 2 : Caco 2 (Human colonic carcinoma cell line) Whole Cell Lysate
      Lane 3 : RAW 264.7 (Mouse leukaemic monocyte macrophage cell line) Whole Cell Lysate
      Lane 4 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate

      Lysates/proteins at 10 µg per lane.

      Developed using the ECL technique.

      Performed under reducing conditions.

      Predicted band size: 152 kDa
      Observed band size: 152 kDa


      Exposure time: 2 minutes


      This blot was produced using a 3-8% Tris Acetate gel under the TA buffer system. The gel was run at 150V for 60 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab197609 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.

    • HRP Anti-Brd4 antibody [EPR5150(2)] (ab197609)
      HRP Anti-Brd4 antibody [EPR5150(2)] (ab197609)

    Protocols

    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
    • SDS

    Certificate of Compliance

    To download a Certificate of Compliance, please enter your Lot number below:

  • References (0)

    Publishing research using ab197609? Please let us know so that we can cite the reference in this datasheet.

    ab197609 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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