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RecombinantRabMAb

Recombinant HRP Anti-Brd4 antibody [EPR5150(2)] (ab197609)

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Western blot - HRP Anti-Brd4 antibody [EPR5150(2)] (ab197609)
  • HRP Anti-Brd4 antibody [EPR5150(2)] (ab197609)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • HRP Rabbit monoclonal [EPR5150(2)] to Brd4
  • Suitable for: WB
  • Reacts with: Mouse, Human
  • Conjugation: HRP

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Alexa Fluor® 594 Alexa Fluor® 647 Carrier Free Unconjugated

Overview

  • Product name

    HRP Anti-Brd4 antibody [EPR5150(2)]
    See all Brd4 primary antibodies

  • Description

    HRP Rabbit monoclonal [EPR5150(2)] to Brd4

  • Host species

    Rabbit

  • Conjugation

    HRP

  • Tested applications

    Suitable for: WBmore details

  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HeLa, CaCo2, RAW264.7 and NIH3T3 whole cell lysates.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab197609 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/5000. Detects a band of approximately 152 kDa (predicted molecular weight: 152 kDa).
Notes
WB
1/5000. Detects a band of approximately 152 kDa (predicted molecular weight: 152 kDa).

Target

  • Function

    Plays a role in a process governing chromosomal dynamics during mitosis.

  • Tissue specificity

    Ubiquitously expressed.

  • Involvement in disease

    Note=A chromosomal aberration involving BRD4 is found in a rare, aggressive, and lethal carcinoma arising in midline organs of young people. Translocation t(15;19)(q14;p13) with NUT which produces a BRD4-NUT fusion protein.

  • Sequence similarities

    Contains 2 bromo domains.

  • Cellular localization

    Nucleus.
  • Information by UniProt

  • Database links

    • Alternative names

      • Brd4 antibody
      • BRD4-NUT FUSION antibody
      • BRD4-NUT fusion oncoprotein antibody
      • BRD4_HUMAN antibody
      • Bromodomain containing 4 antibody
      • bromodomain containing protein 4 antibody
      • Bromodomain-containing protein 4 antibody
      • CAP antibody
      • chromosome associated protein antibody
      • HUNK1 antibody
      • HUNKI antibody
      • MCAP antibody
      • Mitotic chromosome-associated protein antibody
      • Protein HUNK1 antibody
      see all

    Images

    • Western blot - HRP Anti-Brd4 antibody [EPR5150(2)] (ab197609)
      Western blot - HRP Anti-Brd4 antibody [EPR5150(2)] (ab197609)
      All lanes : HRP Anti-Brd4 antibody [EPR5150(2)] (ab197609) at 1/5000 dilution

      Lane 1 : HeLa whole cell lysate (ab150035)
      Lane 2 : Caco 2 (Human colonic carcinoma cell line) Whole Cell Lysate
      Lane 3 : RAW 264.7 (Mouse leukaemic monocyte macrophage cell line) Whole Cell Lysate
      Lane 4 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate

      Lysates/proteins at 10 µg per lane.

      Developed using the ECL technique.

      Performed under reducing conditions.

      Predicted band size: 152 kDa
      Observed band size: 152 kDa


      Exposure time: 2 minutes


      This blot was produced using a 3-8% Tris Acetate gel under the TA buffer system. The gel was run at 150V for 60 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab197609 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.

    • HRP Anti-Brd4 antibody [EPR5150(2)] (ab197609)
      HRP Anti-Brd4 antibody [EPR5150(2)] (ab197609)

    References (0)

    Publishing research using ab197609? Please let us know so that we can cite the reference in this datasheet.

    ab197609 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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