HRP Anti-BrdU antibody [BU1/75 (ICR1)] (ab220507)

Submit a review Submit a question
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-BrdU antibody [BU1/75 (ICR1)] (ab220507)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-BrdU antibody [BU1/75 (ICR1)] (ab220507)

Key features and details

  • HRP Rat monoclonal [BU1/75 (ICR1)] to BrdU
  • Suitable for: IHC-P
  • Reacts with: Species independent
  • Conjugation: HRP
  • Isotype: IgG2a

Overview

  • Product name

    HRP Anti-BrdU antibody [BU1/75 (ICR1)]
    See all BrdU primary antibodies

  • Description

    HRP Rat monoclonal [BU1/75 (ICR1)] to BrdU

  • Host species

    Rat

  • Conjugation

    HRP

  • Tested applications

    Suitable for: IHC-Pmore details

  • Species reactivity

    Reacts with: Species independent

  • Immunogen

    The details of the immunogen for this antibody are not available.

  • Positive control

    • IHC-P: BrdU treated rat skin tissue sections

  • General notes

    The antibody recognises single stranded DNA so the DNA needs to be unraveled first. This can be done with DNAse, although this doesn't give the best results. Depending on the assay, acid denaturation with 2M HCL or heat denaturation are the most successful. Please note this step is critical in any assay with this antibody and is the area that should be modified to optimise results. Detailed BrdU protocol is available in "Neuroscience protocols" on our "Protocol and troubleshooting tips" webpage.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Applications

Our Abpromise guarantee covers the use of ab220507 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Relevance

    The immunocytochemical detection of bromodeoxyuridine (BrdU) incorporated into DNA is a powerful tool to study the cytokinetics of normal and neoplastic cells. In vitro or in vivo labeling of tumor cells with the thymidine analogue BrdU and the subsequent detection of incorporated BrdU with specific anti-BrdU monoclonal antibodies is an accurate and comprehensive method to quantitate the degree of DNA-synthesis. BrdU is incorporated into the newly synthezised DNA of S-phase cells may provide an estimate for the fraction of cells in S-phase. Also dynamic proliferative information such as the S-phase transit rate and the potential doubling time can be obtained, by means of bivariate BrdU/DNA flow cytometric analysis.

  • Cellular localization

    Nuclear

  • Alternative names

    • Bromodeoxyuridine antibody
    • BUdr antibody

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-BrdU antibody [BU1/75 (ICR1)] (ab220507)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-BrdU antibody [BU1/75 (ICR1)] (ab220507)

    IHC image of BrdU staining in a section of formalin-fixed paraffin-embedded BrdU treated rat skin, performed on a Leica BOND™. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab220507, 1/100 dilution, for 15 mins at room temperature. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset negative control image is taken from an identical assay without primary antibody.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-BrdU antibody [BU1/75 (ICR1)] (ab220507)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-BrdU antibody [BU1/75 (ICR1)] (ab220507)

    Negative IHC image of BrdU staining in a section of formalin-fixed paraffin-embedded normal rat skin, performed on a Leica BOND™. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab220507, 1/100 dilution, for 15 mins at room temperature. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset negative control image is taken from an identical assay without primary antibody.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

References (0)

Publishing research using ab220507? Please let us know so that we can cite the reference in this datasheet.

ab220507 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review Submit a question

There are currently no Customer reviews or Questions for ab220507.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com