Recombinant HRP Anti-BRMS1 antibody [EPR7202] (ab208862)
![Western blot - HRP Anti-BRMS1 antibody [EPR7202] (ab208862)](https://www.abcam.com/ps/products/208/ab208862/Images/ab208862-270862-WBab2088621.jpg "Western blot - HRP Anti-BRMS1 antibody [EPR7202] (ab208862)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- HRP Rabbit monoclonal [EPR7202] to BRMS1
- Suitable for: WB
- Reacts with: Human
- Conjugation: HRP
Overview
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Product name
HRP Anti-BRMS1 antibody [EPR7202]
See all BRMS1 primary antibodies -
Description
HRP Rabbit monoclonal [EPR7202] to BRMS1 -
Host species
Rabbit -
Conjugation
HRP -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human BRMS1 aa 1-100 (N terminal). The exact sequence is proprietary.
Database link: Q9HCU9 -
Positive control
- WB: HeLa whole cell lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.1% 10% Proclin 300 Solution
Constituents: PBS, 1% BSA, 30% Glycerol -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR7202 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab208862 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/5000. Detects a band of approximately 33 kDa (predicted molecular weight: 28 kDa). |
Target
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Function
Transcriptional repressor. Down-regulates transcription activation by NF-kappa-B by promoting the deacetylation of RELA at 'Lys-310'. Promotes HDAC1 binding to promoter regions. Down-regulates expression of anti-apoptotic genes that are controlled by NF-kappa-B. Promotes apoptosis in cells that have inadequate adherence to a substrate, a process called anoikis, and may thereby inhibit metastasis. May be a mediator of metastasis suppression in breast carcinoma. -
Tissue specificity
Expression levels are higher in term placentas than in early placentas. Low levels of expression observed in normal pregnancies and in molar pregnancies. -
Sequence similarities
Belongs to the BRMS1 family. -
Cellular localization
Nucleus. Cytoplasm. Predominantly nuclear. - Information by UniProt
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Database links
- Entrez Gene: 25855 Human
- Omim: 606259 Human
- SwissProt: Q9HCU9 Human
- Unigene: 100426 Human
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Alternative names
- AV003220 antibody
- AW554636 antibody
- Breast cancer metastasis suppressor 1 antibody
see all
Images
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Western blot - HRP Anti-BRMS1 antibody [EPR7202] (ab208862)HRP Anti-BRMS1 antibody [EPR7202] (ab208862) at 1/5000 dilution +
HeLa whole cell lysate (ab150035) at 10 µg
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 28 kDa
Observed band size: 33 kDa why is the actual band size different from the predicted?
Exposure time: 20 minutesThis blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab208862 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.
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![HRP Anti-BRMS1 antibody [EPR7202] (ab208862)](https://www.abcam.com/ps/products/208/ab208862/Images/ab208862-2-benefits-of-recombinant-antibodies.png)
HRP Anti-BRMS1 antibody [EPR7202] (ab208862)
References (0)
ab208862 has not yet been referenced specifically in any publications.
