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    hrp-c-myc-antibody-ab19312.pdf

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Epigenetics and Nuclear Signaling Transcription Domain Families HLH / Leucine Zipper HLH / Leucine Zipper
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HRP Anti-c-Myc antibody (ab19312)

  • Datasheet
  • SDS
Reviews (2) Submit a question References (6)

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Immunocytochemistry/ Immunofluorescence - HRP Anti-c-Myc antibody (ab19312)
  • Western blot - HRP Anti-c-Myc antibody (ab19312)

Key features and details

  • HRP Rabbit polyclonal to c-Myc
  • Suitable for: ICC/IF, WB
  • Reacts with: Human
  • Conjugation: HRP
  • Isotype: IgG

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Recombinant human c-Myc protein (Active) (ab169901)
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HRP Anti-Myc tag antibody [9E10] (ab62928)

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Overview

  • Product name

    HRP Anti-c-Myc antibody
    See all c-Myc primary antibodies
  • Description

    HRP Rabbit polyclonal to c-Myc
  • Host species

    Rabbit
  • Conjugation

    HRP
  • Tested applications

    Suitable for: ICC/IF, WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to Human c-Myc aa 410-419 conjugated to keyhole limpet haemocyanin.
    Sequence:

    EQKLISEEDL


    Database link: P01106
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C.
  • Storage buffer

    pH: 6.8
    Constituents: 0.2% BSA, 0.05% CMIT/MIT based preservative
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • Transcription
    • Domain Families
    • HLH / Leucine Zipper
    • HLH / Leucine Zipper
    • Stem Cells
    • Signaling Pathways
    • TGF beta
    • Nuclear
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Transcription Factors
    • Cancer
    • Cell cycle
    • Cell differentiation
    • Cancer
    • Oncoproteins/suppressors
    • Oncoproteins
    • Transcription factors
    • Cancer
    • Tumor biomarkers
    • Oncoproteins

Associated products

  • Assay kits

    • c-Myc Transcription Factor Assay Kit (Colorimetric) (ab207200)
  • Recombinant Protein

    • Recombinant human c-Myc protein (Active) (ab169901)
  • Related Products

    • Human c-Myc peptide (ab166837)
    • TMB ELISA Substrate (Highest Sensitivity) (ab171522)
    • TMB ELISA Substrate (High Sensitivity) (ab171523)
    • TMB ELISA Substrate (Fast Kinetic Rate) (ab171524)
    • TMB ELISA Substrate (Slow Kinetic Rate) (ab171525)
    • TMB ELISA Substrate (Slower Kinetic Rate) (ab171526)
    • TMB ELISA Substrate (Slowest Kinetic Rate) (ab171527)
    • 450 nm Stop Solution for TMB Substrate (ab171529)
    • 650 nm Stop Solution for TMB Substrate (ab171531)
    • Immunoassay Blocking Buffer (ab171534)
    • Immunoassay Blocking (BSA Free) (ab171535)

Applications

Our Abpromise guarantee covers the use of ab19312 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 µg/ml.
WB 1/1000 - 1/10000. Predicted molecular weight: 49 kDa. Colorimetric. Chemiluminescent: 1/1000 - 1/30000.

Target

  • Function

    Participates in the regulation of gene transcription. Binds DNA in a non-specific manner, yet also specifically recognizes the core sequence 5'-CAC[GA]TG-3'. Seems to activate the transcription of growth-related genes.
  • Involvement in disease

    Note=Overexpression of MYC is implicated in the etiology of a variety of hematopoietic tumors.
    Note=A chromosomal aberration involving MYC may be a cause of a form of B-cell chronic lymphocytic leukemia. Translocation t(8;12)(q24;q22) with BTG1.
    Defects in MYC are a cause of Burkitt lymphoma (BL) [MIM:113970]. A form of undifferentiated malignant lymphoma commonly manifested as a large osteolytic lesion in the jaw or as an abdominal mass. Note=Chromosomal aberrations involving MYC are usually found in Burkitt lymphoma. Translocations t(8;14), t(8;22) or t(2;8) which juxtapose MYC to one of the heavy or light chain immunoglobulin gene loci.
  • Sequence similarities

    Contains 1 basic helix-loop-helix (bHLH) domain.
  • Post-translational
    modifications

    Phosphorylated by PRKDC. Phosphorylation at Thr-58 and Ser-62 by GSK3 is required for ubiquitination and degradation by the proteasome.
    Ubiquitinated by the SCF(FBXW7) complex when phosphorylated at Thr-58 and Ser-62, leading to its degradation by the proteasome. In the nucleoplasm, ubiquitination is counteracted by USP28, which interacts with isoform 1 of FBXW7 (FBW7alpha), leading to its deubiquitination and preventing degradation. In the nucleolus, however, ubiquitination is not counteracted by USP28, due to the lack of interaction between isoform 4 of FBXW7 (FBW7gamma) and USP28, explaining the selective MYC degradation in the nucleolus. Also polyubiquitinated by the DCX(TRUSS) complex.
  • Cellular localization

    Nucleus > nucleoplasm. Nucleus > nucleolus.
  • Target information above from: UniProt accession P01106 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 4609 Human
    • Omim: 190080 Human
    • SwissProt: P01106 Human
    • Unigene: 202453 Human
    • Form

      c-Myc is also expressed in the cytoplasm.
    • Alternative names

      • AU016757 antibody
      • Avian myelocytomatosis viral oncogene homolog antibody
      • bHLHe39 antibody
      • c Myc antibody
      • Cellular myelocytomatosis oncogene antibody
      • Class E basic helix-loop-helix protein 39 antibody
      • MGC105490 antibody
      • MRTL antibody
      • Myc antibody
      • Myc protein antibody
      • Myc proto oncogene protein antibody
      • Myc proto-oncogene protein antibody
      • myc-related translation/localization regulatory factor antibody
      • MYC_HUMAN antibody
      • Myc2 antibody
      • myca antibody
      • MYCC antibody
      • Myelocytomatosis oncogene a antibody
      • Myelocytomatosis oncogene antibody
      • Niard antibody
      • Nird antibody
      • oncogene c-Myc antibody
      • Oncogene Myc antibody
      • OTTHUMP00000158589 antibody
      • OTTHUMP00000227763 antibody
      • Proto-oncogene c-Myc antibody
      • Protooncogene homologous to myelocytomatosis virus antibody
      • RNCMYC antibody
      • Transcription factor p64 antibody
      • Transcriptional regulator Myc-A antibody
      • V-Myc avian myelocytomatosis viral oncogene homolog antibody
      • v-myc myelocytomatosis viral oncogene homolog (avian) antibody
      • zc-myc antibody
      see all

    Images

    • Immunocytochemistry/ Immunofluorescence - HRP Anti-c-Myc antibody (ab19312)
      Immunocytochemistry/ Immunofluorescence - HRP Anti-c-Myc antibody (ab19312)
      ICC/IF image of ab19312 stained human HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1% BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab19312, 1 µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue).
    • Western blot - HRP Anti-c-Myc antibody (ab19312)
      Western blot - HRP Anti-c-Myc antibody (ab19312)
      All lanes : HRP Anti-c-Myc antibody (ab19312) at 0.4 µg/ml

      Lane 1 : E. coli whole cell lysate expressing a multi-tag fusion protein at 0.2 µg
      Lane 2 : E. coli whole cell lysate expressing a multi-tag fusion protein at 0.1 µg
      Lane 3 : E. coli whole cell lysate expressing a multi-tag fusion protein at 0.05 µg

      Predicted band size: 49 kDa


      Exposure time: 10 seconds


      Detection: Chemiluminesence

    Protocols

    • Immunocytochemistry & immunofluorescence protocols
    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
    • SDS
  • References (6)

    Publishing research using ab19312? Please let us know so that we can cite the reference in this datasheet.

    ab19312 has been referenced in 6 publications.

    • Wang Q  et al. PIK3CA mutations confer resistance to first-line chemotherapy in colorectal cancer. Cell Death Dis 9:739 (2018). PubMed: 29970892
    • Hayden RS  et al. Cell-tethered ligands modulate bone remodeling by osteoblasts and osteoclasts. Adv Funct Mater 24:472-479 (2014). PubMed: 25419210
    • Boshuizen RS  et al. A combination of in vitro techniques for efficient discovery of functional monoclonal antibodies against human CXC chemokine receptor-2 (CXCR2). MAbs 6:1415-24 (2014). PubMed: 25484047
    • Fortin JP  et al. Discovery of dual-action membrane-anchored modulators of incretin receptors. PLoS One 6:e24693 (2011). ELISA . PubMed: 21935440
    • Fortin JP  et al. Membrane-tethered ligands are effective probes for exploring class B1 G protein-coupled receptor function. Proc Natl Acad Sci U S A 106:8049-54 (2009). ELISA ; Human . PubMed: 19416829
    • Choi C  et al. Cellular dissection of circadian peptide signals with genetically encoded membrane-tethered ligands. Curr Biol 19:1167-75 (2009). ICC/IF . PubMed: 19592252

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-2 of 2 Abreviews or Q&A

    ELISA abreview for Anti-c-Myc antibody (HRP)

    Excellent
    Abreviews
    Abreviews
    Application
    ELISA
    Sample
    Human Recombinant protein (Phage-displayed myc tag scFv)
    Specification
    Phage-displayed myc tag scFv
    Blocking step
    BSA as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
    Type
    Sandwich (Capture)
    Read More

    Abcam user community

    Verified customer

    Submitted Sep 18 2018

    Western blot abreview for Anti-c-Myc antibody (HRP)

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (HEK293)
    Gel Running Conditions
    Reduced Denaturing (12%)
    Loading amount
    10 µg
    Specification
    HEK293
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Dr. Lin Zhu

    Verified customer

    Submitted Sep 08 2017

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