HRP Anti-c-Myc antibody (ab19312)
Key features and details
- HRP Rabbit polyclonal to c-Myc
- Suitable for: ICC/IF, WB
- Reacts with: Human, Recombinant fragment
- Conjugation: HRP
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
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- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
HRP Anti-c-Myc antibody
See all c-Myc primary antibodies -
Description
HRP Rabbit polyclonal to c-Myc -
Host species
Rabbit -
Conjugation
HRP -
Tested applications
Suitable for: ICC/IF, WBmore details -
Species reactivity
Reacts with: Human, Recombinant fragment -
Immunogen
Synthetic peptide corresponding to Human c-Myc aa 410-419 conjugated to keyhole limpet haemocyanin.
Sequence:EQKLISEEDL
Database link: P01106 -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 6.8
Constituents: 0.2% BSA, 0.05% CMIT/MIT based preservative -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Assay kits
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Recombinant Protein
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Related Products
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Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab19312 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF |
Use a concentration of 1 µg/ml.
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| WB | (1) |
1/1000 - 1/10000. Predicted molecular weight: 49 kDa. Colorimetric. Chemiluminescent: 1/1000 - 1/30000.
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| Notes |
|---|
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ICC/IF
Use a concentration of 1 µg/ml. |
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WB
1/1000 - 1/10000. Predicted molecular weight: 49 kDa. Colorimetric. Chemiluminescent: 1/1000 - 1/30000. |
Target
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Function
Participates in the regulation of gene transcription. Binds DNA in a non-specific manner, yet also specifically recognizes the core sequence 5'-CAC[GA]TG-3'. Seems to activate the transcription of growth-related genes. -
Involvement in disease
Note=Overexpression of MYC is implicated in the etiology of a variety of hematopoietic tumors.
Note=A chromosomal aberration involving MYC may be a cause of a form of B-cell chronic lymphocytic leukemia. Translocation t(8;12)(q24;q22) with BTG1.
Defects in MYC are a cause of Burkitt lymphoma (BL) [MIM:113970]. A form of undifferentiated malignant lymphoma commonly manifested as a large osteolytic lesion in the jaw or as an abdominal mass. Note=Chromosomal aberrations involving MYC are usually found in Burkitt lymphoma. Translocations t(8;14), t(8;22) or t(2;8) which juxtapose MYC to one of the heavy or light chain immunoglobulin gene loci. -
Sequence similarities
Contains 1 basic helix-loop-helix (bHLH) domain. -
Post-translational
modificationsPhosphorylated by PRKDC. Phosphorylation at Thr-58 and Ser-62 by GSK3 is required for ubiquitination and degradation by the proteasome.
Ubiquitinated by the SCF(FBXW7) complex when phosphorylated at Thr-58 and Ser-62, leading to its degradation by the proteasome. In the nucleoplasm, ubiquitination is counteracted by USP28, which interacts with isoform 1 of FBXW7 (FBW7alpha), leading to its deubiquitination and preventing degradation. In the nucleolus, however, ubiquitination is not counteracted by USP28, due to the lack of interaction between isoform 4 of FBXW7 (FBW7gamma) and USP28, explaining the selective MYC degradation in the nucleolus. Also polyubiquitinated by the DCX(TRUSS) complex. -
Cellular localization
Nucleus > nucleoplasm. Nucleus > nucleolus. - Information by UniProt
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Database links
- Entrez Gene: 4609 Human
- Omim: 190080 Human
- SwissProt: P01106 Human
- Unigene: 202453 Human
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Form
c-Myc is also expressed in the cytoplasm. -
Alternative names
- AU016757 antibody
- Avian myelocytomatosis viral oncogene homolog antibody
- bHLHe39 antibody
see all
Images
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Immunocytochemistry/ Immunofluorescence - HRP Anti-c-Myc antibody (ab19312)ICC/IF image of ab19312 stained human HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1% BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab19312, 1 µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue).
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Western blot - HRP Anti-c-Myc antibody (ab19312)All lanes : HRP Anti-c-Myc antibody (ab19312) at 0.4 µg/ml
Lane 1 : E. coli whole cell lysate expressing a multi-tag fusion protein at 0.2 µg
Lane 2 : E. coli whole cell lysate expressing a multi-tag fusion protein at 0.1 µg
Lane 3 : E. coli whole cell lysate expressing a multi-tag fusion protein at 0.05 µg
Predicted band size: 49 kDa
Exposure time: 10 secondsDetection: Chemiluminesence
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (6)
ab19312 has been referenced in 6 publications.
- Wang Q et al. PIK3CA mutations confer resistance to first-line chemotherapy in colorectal cancer. Cell Death Dis 9:739 (2018). PubMed: 29970892
- Hayden RS et al. Cell-tethered ligands modulate bone remodeling by osteoblasts and osteoclasts. Adv Funct Mater 24:472-479 (2014). PubMed: 25419210
- Boshuizen RS et al. A combination of in vitro techniques for efficient discovery of functional monoclonal antibodies against human CXC chemokine receptor-2 (CXCR2). MAbs 6:1415-24 (2014). PubMed: 25484047
- Fortin JP et al. Discovery of dual-action membrane-anchored modulators of incretin receptors. PLoS One 6:e24693 (2011). ELISA . PubMed: 21935440
- Fortin JP et al. Membrane-tethered ligands are effective probes for exploring class B1 G protein-coupled receptor function. Proc Natl Acad Sci U S A 106:8049-54 (2009). ELISA ; Human . PubMed: 19416829
- Choi C et al. Cellular dissection of circadian peptide signals with genetically encoded membrane-tethered ligands. Curr Biol 19:1167-75 (2009). ICC/IF . PubMed: 19592252