HRP Anti-CaMKII antibody [6G9] (ab197500)

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Western blot - HRP Anti-CaMKII antibody [6G9] (ab197500)

    Key features and details

    • HRP Mouse monoclonal [6G9] to CaMKII
    • Suitable for: WB
    • Reacts with: Mouse, Rat, Human
    • Conjugation: HRP
    • Isotype: IgG1

    Overview

    • Product name

      HRP Anti-CaMKII antibody [6G9]
      See all CaMKII primary antibodies

    • Description

      HRP Mouse monoclonal [6G9] to CaMKII

    • Host species

      Mouse

    • Conjugation

      HRP

    • Tested applications

      Suitable for: WBmore details

    • Species reactivity

      Reacts with: Mouse, Rat, Human
      Predicted to work with: Cow

    • Immunogen

      Full length native protein (purified) corresponding to Rat CaMKII.

    • Positive control

      • WB: Rat, mouse and human brain tissue lysates.

    • General notes

      Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

      Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

      We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

      In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

      We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

      Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

      Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

    Properties

    Applications

    Our Abpromise guarantee covers the use of ab197500 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    WB 1/5000. Detects a band of approximately 50 kDa (predicted molecular weight: 56 kDa).

    Target

    Images

    • Western blot - HRP Anti-CaMKII antibody [6G9] (ab197500)
      Western blot - HRP Anti-CaMKII antibody [6G9] (ab197500)
      All lanes : HRP Anti-CaMKII antibody [6G9] (ab197500) at 1/5000 dilution

      Lane 1 : Brain (Rat) Tissue Lysate
      Lane 2 : Brain (Mouse) Tissue Lysate
      Lane 3 : Brain (Human) Tissue Lysate - adult normal tissue

      Lysates/proteins at 20 µg per lane.

      Developed using the ECL technique.

      Performed under reducing conditions.

      Predicted band size: 56 kDa
      Observed band size: 50 kDa
      why is the actual band size different from the predicted?


      Exposure time: 30 seconds


      This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab197500 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.

    References (0)

    Publishing research using ab197500? Please let us know so that we can cite the reference in this datasheet.

    ab197500 has not yet been referenced specifically in any publications.

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