Key features and details
- HRP Mouse monoclonal [6G9] to CaMKII
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
- Conjugation: HRP
- Isotype: IgG1
Product nameHRP Anti-CaMKII antibody [6G9]
See all CaMKII primary antibodies
DescriptionHRP Mouse monoclonal [6G9] to CaMKII
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Mouse, Rat, Human
Predicted to work with: Cow
Full length native protein (purified) corresponding to Rat CaMKII.
- WB: Rat, mouse and human brain tissue lysates.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark.
Storage bufferpH: 7.40
Preservative: 0.1% 10% Proclin 300 Solution
Constituents: 30% Glycerol, 1% BSA, PBS
Concentration information loading...
Our Abpromise guarantee covers the use of ab197500 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/5000. Detects a band of approximately 50 kDa (predicted molecular weight: 56 kDa).|
FunctionCaM-kinase II (CAMK2) is a prominent kinase in the central nervous system that may function in long-term potentiation and neurotransmitter release. Member of the NMDAR signaling complex in excitatory synapses it may regulate NMDAR-dependent potentiation of the AMPAR and synaptic plasticity.
Sequence similaritiesBelongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CaMK subfamily.
Contains 1 protein kinase domain.
Cellular localizationCell junction > synapse > presynaptic cell membrane. Cell junction > synapse. Postsynaptic lipid rafts.
- Information by UniProt
- Calcium/calmodulin dependent protein kinase II alpha antibody
- Calcium/calmodulin dependent protein kinase II beta antibody
- Calcium/calmodulin dependent protein kinase II delta antibody
All lanes : HRP Anti-CaMKII antibody [6G9] (ab197500) at 1/5000 dilution
Lane 1 : Brain (Rat) Tissue Lysate
Lane 2 : Brain (Mouse) Tissue Lysate
Lane 3 : Brain (Human) Tissue Lysate - adult normal tissue
Lysates/proteins at 20 µg per lane.
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 56 kDa
Observed band size: 50 kDa why is the actual band size different from the predicted?
Exposure time: 30 seconds
This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab197500 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.
ab197500 has not yet been referenced specifically in any publications.