Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- HRP Rabbit monoclonal [EPR12927] to Caspase-14
- Suitable for: WB
- Reacts with: Human
- Conjugation: HRP
Product nameHRP Anti-Caspase-14 antibody [EPR12927]
See all Caspase-14 primary antibodies
DescriptionHRP Rabbit monoclonal [EPR12927] to Caspase-14
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
Synthetic peptide within Human Caspase-14 aa 100-200 (internal sequence). The exact sequence is proprietary.
Database link: P31944
- WB: Human skin lysate.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Store In the Dark.
Storage bufferpH: 7.40
Preservative: 0.1% 10% Proclin 300 Solution
Constituents: PBS, 30% Glycerol, 1% BSA
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab200079 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/5000. Detects a band of approximately 28 kDa (predicted molecular weight: 28 kDa).|
FunctionBelieved to be a non-apoptotic caspase which is involved in epidermal differentiation. Seems to play a role in keratinocyte differentiation and cornification. Probably regulates maturation of the epidermis by proteolytically processing filaggrin.
Tissue specificityExpressed in keratinocytes of adult skin suprabasal layers (from spinous layers to the stratum granulosum and stratum corneum) (at protein level). Expressed in keratinocytes of hair shaft and sebaceous glands (at protein level). In psoriatic skin only expressed at very low levels.
Sequence similaritiesBelongs to the peptidase C14A family.
Cellular localizationCytoplasm. Nucleus.
- Information by UniProt
- Apoptosis related cysteine protease antibody
- CASP 14 antibody
- CASP-14 antibody
HRP Anti-Caspase-14 antibody [EPR12927] (ab200079) at 1/5000 dilution + Skin (Human) Tissue Lysate - adult normal tissue at 10 µg
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 28 kDa
Observed band size: 28 kDa
Exposure time: 20 minutes
This blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab200079 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.
ab200079 has not yet been referenced specifically in any publications.