HRP Anti-Cathepsin L + V antibody [33/2] (ab197278)

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Western blot - HRP Anti-Cathepsin L + V antibody [33/2] (ab197278)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-Cathepsin L + V antibody [33/2] (ab197278)

Key features and details

  • HRP Mouse monoclonal [33/2] to Cathepsin L + V
  • Suitable for: IHC-P, WB
  • Reacts with: Human
  • Conjugation: HRP
  • Isotype: IgG1

Overview

  • Product name

    HRP Anti-Cathepsin L + V antibody [33/2]
    See all Cathepsin L + V primary antibodies

  • Description

    HRP Mouse monoclonal [33/2] to Cathepsin L + V

  • Host species

    Mouse

  • Conjugation

    HRP

  • Tested applications

    Suitable for: IHC-P, WBmore details

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat

  • Immunogen

    Full length native protein (purified) corresponding to Human Cathepsin L + V.

  • Epitope

    Recognizes an epitope within amino acid residues GYGFEST (265-271 in procathepsin L and 169-175 in the mature cathepsin L molecule).

  • Positive control

    • WB: Human lung and kidney (normal) tissue lysates. IHC-P: Normal human kidney tissue.

  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Applications

Our Abpromise guarantee covers the use of ab197278 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/5000. Detects a band of approximately 25 kDa (predicted molecular weight: 38 kDa).

Abcam recommends using 3% milk as the blocking agent.

Images

  • Western blot - HRP Anti-Cathepsin L + V antibody [33/2] (ab197278)
    Western blot - HRP Anti-Cathepsin L + V antibody [33/2] (ab197278)
    All lanes : HRP Anti-Cathepsin L + V antibody [33/2] (ab197278) at 1/5000 dilution

    Lane 1 : Lung (Human) Tissue Lysate - adult normal tissue
    Lane 2 : Human kidney tissue lysate - total protein (ab30203)

    Lysates/proteins at 20 µg per lane.

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 38 kDa
    Observed band size: 25 kDa
    why is the actual band size different from the predicted?


    Exposure time: 4 minutes


    This blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab197278 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-Cathepsin L + V antibody [33/2] (ab197278)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-Cathepsin L + V antibody [33/2] (ab197278)

    IHC image of Cathepsin L + V staining in a section of formalin-fixed paraffin-embedded normal human kidney*, performed on a Leica BOND. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab197278 at 1/500 dilution, for 15 mins at room temperature. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset negative control image is taken from an identical assay without primary antibody.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

References (0)

Publishing research using ab197278? Please let us know so that we can cite the reference in this datasheet.

ab197278 has not yet been referenced specifically in any publications.

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