Recombinant HRP Anti-CCR7 antibody [Y59] (ab196475)
![Western blot - HRP Anti-CCR7 antibody [Y59] (ab196475)](https://www.abcam.com/ps/products/196/ab196475/Images/ab196475-388316-196475jfle0632westernblotc6raw2647.jpg "Western blot - HRP Anti-CCR7 antibody [Y59] (ab196475)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- HRP Rabbit monoclonal [Y59] to CCR7
- Suitable for: WB
- Reacts with: Mouse, Rat
- Conjugation: HRP
Overview
-
Product name
HRP Anti-CCR7 antibody [Y59]
See all CCR7 primary antibodies -
Description
HRP Rabbit monoclonal [Y59] to CCR7 -
Host species
Rabbit -
Conjugation
HRP -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse, Rat
Predicted to work with: Human
-
Immunogen
Synthetic peptide within Human CCR7 (N terminal). The exact sequence is proprietary.
-
Positive control
- WB: C6 and Raw264.7 whole cell lysates.
-
General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.1% 10% Proclin 300 Solution
Constituents: 30% Glycerol, 1% BSA, PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
Y59 -
Isotype
IgG -
Research areas
Associated products
-
Alternative Versions
- Alexa Fluor® 488 Anti-CCR7 antibody [Y59] (ab196459)
- Alexa Fluor® 647 Anti-CCR7 antibody [Y59] (ab196640)
- Alexa Fluor® 594 Anti-CCR7 antibody [Y59] (ab207016)
- Alexa Fluor® 555 Anti-CCR7 antibody [Y59] (ab207018)
- Alexa Fluor® 568 Anti-CCR7 antibody [Y59] (ab207019)
- Anti-CCR7 antibody [Y59] - Low endotoxin, Azide free (ab221209)
- Anti-CCR7 antibody [Y59] (ab32527)
-
Isotype control
-
Positive Controls
-
Related Products
Applications
Our Abpromise guarantee covers the use of ab196475 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/5000. Detects a band of approximately 43 kDa (predicted molecular weight: 43 kDa). This product is not recommended for use with human samples in western blot. |
Target
-
Function
Receptor for the MIP-3-beta chemokine. Probable mediator of EBV effects on B-lymphocytes or of normal lymphocyte functions. -
Tissue specificity
Expressed in various lymphoid tissues and activated B- and T-lymphocytes, strongly up-regulated in B-cells infected with Epstein-Barr virus and T-cells infected with herpesvirus 6 or 7. -
Sequence similarities
Belongs to the G-protein coupled receptor 1 family. -
Cellular localization
Cell membrane. - Information by UniProt
-
Database links
- Entrez Gene: 1236 Human
- Entrez Gene: 12775 Mouse
- Entrez Gene: 287673 Rat
- Omim: 600242 Human
- SwissProt: P32248 Human
- SwissProt: P47774 Mouse
- Unigene: 370036 Human
- Unigene: 2932 Mouse
-
Alternative names
- BLR 2 antibody
- BLR2 antibody
- C C chemokine receptor type 7 antibody
see all
Images
-
Western blot - HRP Anti-CCR7 antibody [Y59] (ab196475)All lanes : HRP Anti-CCR7 antibody [Y59] (ab196475) at 1/5000 dilution (Recombinant Anti-CCR7 antibody [Y59] (HRP))
Lane 1 : C6 (rat glial tumor cell line) cell lysate
Lane 2 : RAW 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 43 kDa
Exposure time: 10 secondsab196475 was shown to react with CCR7 in western blot. Membranes were blocked in 2% BSA in TBS-T (0.1% Tween®) before incubation with ab196475 overnight at 4°C at a 1 in 5000 dilution. Blots were incubated with secondary antibody at 1/5000 for 1 hour at room temperature before development with Optiblot ECL reagent (ab133456) and imaging.
-
![HRP Anti-CCR7 antibody [Y59] (ab196475)](https://www.abcam.com/ps/products/196/ab196475/Images/ab196475-4-benefits-of-recombinant-antibodies.png)
HRP Anti-CCR7 antibody [Y59] (ab196475)
References (0)
ab196475 has not yet been referenced specifically in any publications.
