RecombinantRabMAb

Recombinant HRP Anti-CCR7 antibody [Y59] (ab196475)

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Western blot - HRP Anti-CCR7 antibody [Y59] (ab196475)
  • HRP Anti-CCR7 antibody [Y59] (ab196475)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • HRP Rabbit monoclonal [Y59] to CCR7
  • Suitable for: WB
  • Reacts with: Mouse, Rat
  • Conjugation: HRP

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Alexa Fluor® 555 Alexa Fluor® 568 Alexa Fluor® 594 Alexa Fluor® 647 Carrier Free Unconjugated

Overview

  • Product name

    HRP Anti-CCR7 antibody [Y59]
    See all CCR7 primary antibodies

  • Description

    HRP Rabbit monoclonal [Y59] to CCR7

  • Host species

    Rabbit

  • Conjugation

    HRP

  • Tested applications

    Suitable for: WBmore details

  • Species reactivity

    Reacts with: Mouse, Rat
    Predicted to work with: Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: C6 and Raw264.7 whole cell lysates.

  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab196475 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/5000. Detects a band of approximately 43 kDa (predicted molecular weight: 43 kDa).

This product is not recommended for use with human samples in western blot.
Notes
WB
1/5000. Detects a band of approximately 43 kDa (predicted molecular weight: 43 kDa).

This product is not recommended for use with human samples in western blot.

Target

  • Function

    Receptor for the MIP-3-beta chemokine. Probable mediator of EBV effects on B-lymphocytes or of normal lymphocyte functions.

  • Tissue specificity

    Expressed in various lymphoid tissues and activated B- and T-lymphocytes, strongly up-regulated in B-cells infected with Epstein-Barr virus and T-cells infected with herpesvirus 6 or 7.

  • Sequence similarities

    Belongs to the G-protein coupled receptor 1 family.

  • Cellular localization

    Cell membrane.
  • Information by UniProt

  • Database links

    • Alternative names

      • BLR 2 antibody
      • BLR2 antibody
      • C C chemokine receptor type 7 antibody
      • C C CKR 7 antibody
      • C-C chemokine receptor type 7 antibody
      • C-C CKR-7 antibody
      • CC chemokine receptor 7 antibody
      • CC chemokine receptor type 7 antibody
      • CC CKR 7 antibody
      • CC-CKR-7 antibody
      • CCCKR7 antibody
      • CCR 7 antibody
      • CCR-7 antibody
      • Ccr7 antibody
      • CCR7_HUMAN antibody
      • CD 197 antibody
      • CD197 antibody
      • CD197 antigen antibody
      • CDw197 antibody
      • Chemokine (C C motif) receptor 7 antibody
      • Chemokine (C C) receptor 7 antibody
      • Chemokine C C motif receptor 7 antibody
      • Chemokine C C receptor 7 antibody
      • Chemokine receptor 7 antibody
      • Chemokine receptor 7-like protein antibody
      • CMKBR7 antibody
      • EBI 1 antibody
      • EBI1 antibody
      • Ebi1h antibody
      • EBV Induced G Protein Coupled Receptor 1 antibody
      • EBV-induced G-protein coupled receptor 1 antibody
      • Epstein Barr virus induced G protein coupled receptor antibody
      • Epstein Barr virus induced gene 1 antibody
      • Epstein-Barr virus-induced G-protein coupled receptor 1 antibody
      • EVI 1 antibody
      • EVI1 antibody
      • Lymphocyte Specific G Protein Coupled Peptide Receptor antibody
      • MGC108519 antibody
      • MIP 3 beta receptor antibody
      • MIP-3 beta receptor antibody
      • MIP3 Beta Receptor antibody
      see all

    Images

    • Western blot - HRP Anti-CCR7 antibody [Y59] (ab196475)
      Western blot - HRP Anti-CCR7 antibody [Y59] (ab196475)
      All lanes : HRP Anti-CCR7 antibody [Y59] (ab196475) at 1/5000 dilution (Recombinant Anti-CCR7 antibody [Y59] (HRP))

      Lane 1 : C6 (rat glial tumor cell line) cell lysate
      Lane 2 : RAW 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) cell lysate

      Lysates/proteins at 20 µg per lane.

      Performed under reducing conditions.

      Predicted band size: 43 kDa


      Exposure time: 10 seconds


      ab196475 was shown to react with CCR7 in western blot.  Membranes were blocked in 2% BSA in TBS-T (0.1% Tween®) before incubation with ab196475 overnight at 4°C at a 1 in 5000 dilution. Blots were incubated with  secondary antibody at 1/5000 for 1 hour at room temperature before development with Optiblot ECL reagent (ab133456) and imaging.

    • HRP Anti-CCR7 antibody [Y59] (ab196475)
      HRP Anti-CCR7 antibody [Y59] (ab196475)

    References (0)

    Publishing research using ab196475? Please let us know so that we can cite the reference in this datasheet.

    ab196475 has not yet been referenced specifically in any publications.

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