For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome

Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online
United States
Your country/region is currently set to:

If incorrect, please enter your country/region into the box below, to view site information related to your country/region.

Call (888) 77-ABCAM (22226) or contact us
Need help? Contact us

  • My account
  • Sign out
Sign in or Register with us

Welcome

Sign in or

Don't have an account?

Register with us
My basket
Quick order
Abcam homepage

  • Research Products
    By product type
    Primary antibodies
    Secondary antibodies
    ELISA and Matched Antibody Pair Kits
    Cell and tissue imaging tools
    Cellular and biochemical assays
    Proteins and Peptides
    By product type
    Proteomics tools
    Agonists, activators, antagonists and inhibitors
    Cell lines and Lysates
    Multiplex miRNA assays
    Multiplex Assays
    By research area
    Cancer
    Cardiovascular
    Cell Biology
    Epigenetics
    Metabolism
    Developmental Biology
    By research area
    Immunology
    Microbiology
    Neuroscience
    Signal Transduction
    Stem Cells
  • Customized Products & Partnerships
    Customized Products & Partnerships

    Customized products and commercial partnerships to accelerate your diagnostic and therapeutic programs.

    Customized products

    Partner with us

  • Support
    Support hub

    Access advice and support for any research roadblock

    View support hub

    Protocols

    Your experiments laid out step by step

    View protocols

  • Events
    • Conference calendar
    • Cancer
    • Cardiovascular
    • Epigenetics & Nuclear signaling
    • Immunology
    • Neuroscience
    • Stem cells
    • Tradeshows
    • Scientific webinars
    Keep up to date with the latest events

    Full event breakdown with abstracts, speakers, registration and more

    View global event calendar

  • Pathways
    Cell signalling pathways

    View all pathways

    View all interactive pathways

  1. Link

    hrp-cd11b-antibody-epr1344-ab204472.pdf

  1. Send me a copy of this email
    I agree to the terms and conditions.
Signal Transduction Cytoskeleton / ECM Cell Adhesion Integrins Alpha
Share by email
RecombinantRabMAb

Recombinant HRP Anti-CD11b antibody [EPR1344] (ab204472)

  • Datasheet
  • SDS
  • Certificate of Compliance
Submit a review Submit a question

Product price, shipping and contact information

Currently unavailable

Sorry, we can't display this right now.

Please contact us to place your order, or try again later.

 

Loading size & price…

 

Shipping and order information

Shipping info

Promotion Information

Abpromise

Guaranteed product quality, expert customer support.

Find out more.

Western blot - HRP Anti-CD11b antibody [EPR1344] (ab204472)
  • Western blot - HRP Anti-CD11b antibody [EPR1344] (ab204472)
  • HRP Anti-CD11b antibody [EPR1344] (ab204472)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • HRP Rabbit monoclonal [EPR1344] to CD11b
  • Suitable for: WB
  • Reacts with: Mouse, Human
  • Conjugation: HRP

Conjugates logo Related conjugates and formulations

Carrier Free Unconjugated

You may also be interested in

Conjugation
Product image
HRP Conjugation Kit - Lightning-Link® (ab102890)
Protein
Recombinant Human CD11b protein (ab126010)
Primary
Product image
Anti-CD11b antibody [SP331] - C-terminal (ab224800)

View more associated products

Overview

  • Product name

    HRP Anti-CD11b antibody [EPR1344]
    See all CD11b primary antibodies
  • Description

    HRP Rabbit monoclonal [EPR1344] to CD11b
  • Host species

    Rabbit
  • Conjugation

    HRP
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide within Human CD11b. The exact sequence is proprietary.
    Database link: P11215

  • Positive control

    • WB: THP-1 whole cell lysate + TPA, Raw264.7 whole cell lysate and mouse spleen tissue lysate.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark.
  • Storage buffer

    pH: 7.40
    Preservative: 0.1% Proclin 300 Solution
    Constituents: 30% Glycerol (glycerin, glycerine), 1% BSA, PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR1344
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Cytoskeleton / ECM
    • Cell Adhesion
    • Integrins
    • Alpha
    • Tags & Cell Markers
    • Cell Type Markers
    • Neuroscience Markers
    • Glial
    • Neuroscience
    • Cell Type Marker
    • Glia marker
    • Microglia marker
    • Stem Cells
    • Mesenchymal Stem Cells
    • Negative Markers
    • Stem Cells
    • Hematopoietic Progenitors
    • Hematopoietic Stem Cells
    • Murine Lineage Negative
    • Stem Cells
    • Hematopoietic Progenitors
    • Lymphoid
    • B Lymphocytic Lineage
    • Stem Cells
    • Hematopoietic Progenitors
    • Lymphoid
    • T Lymphocytic Lineage
    • Stem Cells
    • Hematopoietic Progenitors
    • Lymphoid
    • NK Cell Lineage
    • Stem Cells
    • Hematopoietic Progenitors
    • Myeloid
    • Monocytic Lineage
    • Stem Cells
    • Hematopoietic Progenitors
    • Myeloid
    • Neutrophil Lineage
    • Neuroscience
    • Development

Associated products

  • Alternative Versions

    • Anti-CD11b antibody [EPR1344] (ab133357)
    • Anti-CD11b antibody [EPR1344] - BSA and Azide free (ab209970)
    • Anti-CD11b antibody [EPR1344] - Low endotoxin, Azide free (ab216445)
  • Isotype control

    • HRP Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab199507)
  • Recombinant Protein

    • Recombinant Human CD11b protein (ab126010)
  • Related Products

    • ECL Substrate Kit (High Sensitivity) (ab133406)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab204472 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/5000. Detects a band of approximately 175 kDa (predicted molecular weight: 127 kDa).
Notes
WB
1/5000. Detects a band of approximately 175 kDa (predicted molecular weight: 127 kDa).

Target

  • Function

    Integrin alpha-M/beta-2 is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated particles. It is identical with CR-3, the receptor for the iC3b fragment of the third complement component. It probably recognizes the R-G-D peptide in C3b. Integrin alpha-M/beta-2 is also a receptor for fibrinogen, factor X and ICAM1. It recognizes P1 and P2 peptides of fibrinogen gamma chain.
  • Tissue specificity

    Predominantly expressed in monocytes and granulocytes.
  • Involvement in disease

    Genetic variations in ITGAM has been associated with susceptibility to systemic lupus erythematosus type 6 (SLEB6) [MIM:609939]. Systemic lupus erythematosus (SLE) is a chronic, inflammatory and often febrile multisystemic disorder of connective tissue. It affects principally the skin, joints, kidneys and serosal membranes. It is thought to represent a failure of the regulatory mechanisms of the autoimmune system.
  • Sequence similarities

    Belongs to the integrin alpha chain family.
    Contains 7 FG-GAP repeats.
    Contains 1 VWFA domain.
  • Domain

    The integrin I-domain (insert) is a VWFA domain. Integrins with I-domains do not undergo protease cleavage.
  • Cellular localization

    Membrane.
  • Target information above from: UniProt accession P11215 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 3684 Human
    • Entrez Gene: 16409 Mouse
    • Omim: 120980 Human
    • SwissProt: P11215 Human
    • SwissProt: P05555 Mouse
    • Unigene: 172631 Human
    • Unigene: 262106 Mouse
    • Alternative names

      • antigen CD11b (p170) antibody
      • Antigen CD11b p170 antibody
      • CD11 antigen like family member B antibody
      • CD11 antigen-like family member B antibody
      • CD11b antibody
      • CD11b/CD18 antibody
      • CD49d antibody
      • Cell surface glycoprotein MAC-1 subunit alpha antibody
      • Complement component 3 receptor 3 subunit antibody
      • Complement Component Receptor 3 Alpha antibody
      • Complement receptor type 3 antibody
      • Complement receptor type 3, alpha subunit antibody
      • CR 3 alpha chain (CR3A) antibody
      • CR 3 alpha chain antibody
      • CR-3 alpha chain antibody
      • CR3 antibody
      • CR3A antibody
      • F730045J24Rik antibody
      • Integrin Alpha M antibody
      • Integrin alpha M chain antibody
      • Integrin alpha-M antibody
      • Integrin beta 2 alpha subunit antibody
      • Integrin subunit alpha M antibody
      • integrin, alpha M (complement component 3 receptor 3 subunit) antibody
      • ITAM_HUMAN antibody
      • ITGAM antibody
      • Leukocyte adhesion receptor MO1 antibody
      • Ly-40 antibody
      • MAC 1 antibody
      • Mac-1a antibody
      • MAC1 antibody
      • Mac1, alpha subunit antibody
      • MAC1A antibody
      • Macrophage antigen alpha polypeptide antibody
      • MGC117044 antibody
      • Mo1 antibody
      • Mo1, alpha subunit antibody
      • MO1A antibody
      • Neutrophil adherence receptor alpha M subunit antibody
      • Neutrophil adherence receptor antibody
      • SLEB6 antibody
      see all

    Images

    • Western blot - HRP Anti-CD11b antibody [EPR1344] (ab204472)
      Western blot - HRP Anti-CD11b antibody [EPR1344] (ab204472)
      All lanes : HRP Anti-CD11b antibody [EPR1344] (ab204472) at 1/5000 dilution

      Lane 1 : RAW 264.7 (Mouse leukaemic monocyte macrophage cell line) Whole Cell Lysate
      Lane 2 : Spleen (Mouse) Tissue Lysate

      Lysates/proteins at 10 µg per lane.

      Developed using the ECL technique.

      Performed under reducing conditions.

      Predicted band size: 127 kDa
      Observed band size: 175 kDa why is the actual band size different from the predicted?


      Exposure time: 12 minutes


      This blot was produced using a 3-8% Tris Acetate gel under the TA buffer system. The gel was run at 150V for 60 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab204472 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.

    • Western blot - HRP Anti-CD11b antibody [EPR1344] (ab204472)
      Western blot - HRP Anti-CD11b antibody [EPR1344] (ab204472)
      HRP Anti-CD11b antibody [EPR1344] (ab204472) at 1/5000 dilution + THP1 (Human acute monocytic leukemia cell line) Whole Cell Lysate + TPA at 10 µg

      Developed using the ECL technique.

      Performed under reducing conditions.

      Predicted band size: 127 kDa
      Observed band size: 175 kDa why is the actual band size different from the predicted?


      Exposure time: 20 minutes


      This blot was produced using a 3-8% Tris Acetate gel under the TA buffer system. The gel was run at 150V for 60 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab204472 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.

    • HRP Anti-CD11b antibody [EPR1344] (ab204472)
      HRP Anti-CD11b antibody [EPR1344] (ab204472)

    Protocols

    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    Certificate of Compliance

    To download a Certificate of Compliance, please enter your Lot number below:

    References (0)

    Publishing research using ab204472? Please let us know so that we can cite the reference in this datasheet.

    ab204472 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    There are currently no Customer reviews or Questions for ab204472.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    Get resources and offers direct to your inbox Sign up
    A-Z by research area
    • Cancer
    • Cardiovascular
    • Cell biology
    • Developmental biology
    • Epigenetics & Nuclear signaling
    • Immunology
    • Metabolism
    • Microbiology
    • Neuroscience
    • Signal transduction
    • Stem cells
    A-Z by product type
    • Primary antibodies
    • Secondary antibodies
    • Biochemicals
    • Isotype controls
    • Flow cytometry multi-color selector
    • Kits
    • Loading controls
    • Lysates
    • Peptides
    • Proteins
    • Slides
    • Tags and cell markers
    • Tools & Reagents
    Help & support
    • Support
    • Make an Inquiry
    • Protocols & troubleshooting
    • Placing an order
    • RabMAb products
    • Biochemical product FAQs
    • Training
    • Browse by Target
    Company
    • Corporate site
    • Investor relations
    • Company news
    • Careers
    • About us
    • Blog
    Events
    • Tradeshows
    • Conferences
    International websites
    • abcam.cn
    • abcam.co.jp

    Join with us

    • LinkedIn
    • facebook
    • Twitter
    • YouTube
    • Terms of sale
    • Website terms of use
    • Cookie policy
    • Privacy policy
    • Legal
    • Modern slavery statement
    © 1998-2022 Abcam plc. All rights reserved.