Recombinant HRP Anti-CD11b antibody [EPR1344] (ab204472)
![Western blot - HRP Anti-CD11b antibody [EPR1344] (ab204472)](https://www.abcam.com/ps/products/204/ab204472/Images/ab204472-252497-anti-cd11b-antibody-epr1344-hrp-western-blot.jpg "Western blot - HRP Anti-CD11b antibody [EPR1344] (ab204472)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- HRP Rabbit monoclonal [EPR1344] to CD11b
- Suitable for: WB
- Reacts with: Mouse, Human
- Conjugation: HRP
Related conjugates and formulations
Overview
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Product name
HRP Anti-CD11b antibody [EPR1344]
See all CD11b primary antibodies -
Description
HRP Rabbit monoclonal [EPR1344] to CD11b -
Host species
Rabbit -
Conjugation
HRP -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Synthetic peptide within Human CD11b. The exact sequence is proprietary.
Database link: P11215 -
Positive control
- WB: THP-1 whole cell lysate + TPA, Raw264.7 whole cell lysate and mouse spleen tissue lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.1% Proclin 300 Solution
Constituents: 30% Glycerol (glycerin, glycerine), 1% BSA, PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR1344 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab204472 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/5000. Detects a band of approximately 175 kDa (predicted molecular weight: 127 kDa).
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| Notes |
|---|
|
WB
1/5000. Detects a band of approximately 175 kDa (predicted molecular weight: 127 kDa). |
Target
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Function
Integrin alpha-M/beta-2 is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated particles. It is identical with CR-3, the receptor for the iC3b fragment of the third complement component. It probably recognizes the R-G-D peptide in C3b. Integrin alpha-M/beta-2 is also a receptor for fibrinogen, factor X and ICAM1. It recognizes P1 and P2 peptides of fibrinogen gamma chain. -
Tissue specificity
Predominantly expressed in monocytes and granulocytes. -
Involvement in disease
Genetic variations in ITGAM has been associated with susceptibility to systemic lupus erythematosus type 6 (SLEB6) [MIM:609939]. Systemic lupus erythematosus (SLE) is a chronic, inflammatory and often febrile multisystemic disorder of connective tissue. It affects principally the skin, joints, kidneys and serosal membranes. It is thought to represent a failure of the regulatory mechanisms of the autoimmune system. -
Sequence similarities
Belongs to the integrin alpha chain family.
Contains 7 FG-GAP repeats.
Contains 1 VWFA domain. -
Domain
The integrin I-domain (insert) is a VWFA domain. Integrins with I-domains do not undergo protease cleavage. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 3684 Human
- Entrez Gene: 16409 Mouse
- Omim: 120980 Human
- SwissProt: P11215 Human
- SwissProt: P05555 Mouse
- Unigene: 172631 Human
- Unigene: 262106 Mouse
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Alternative names
- antigen CD11b (p170) antibody
- Antigen CD11b p170 antibody
- CD11 antigen like family member B antibody
see all
Images
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Western blot - HRP Anti-CD11b antibody [EPR1344] (ab204472)All lanes : HRP Anti-CD11b antibody [EPR1344] (ab204472) at 1/5000 dilution
Lane 1 : RAW 264.7 (Mouse leukaemic monocyte macrophage cell line) Whole Cell Lysate
Lane 2 : Spleen (Mouse) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 127 kDa
Observed band size: 175 kDa why is the actual band size different from the predicted?
Exposure time: 12 minutesThis blot was produced using a 3-8% Tris Acetate gel under the TA buffer system. The gel was run at 150V for 60 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab204472 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.
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![Western blot - HRP Anti-CD11b antibody [EPR1344] (ab204472)](https://www.abcam.com/ps/products/204/ab204472/Images/ab204472-252496-anti-cd11b-antibody-epr1344-hrp-western-blot.jpg)
Western blot - HRP Anti-CD11b antibody [EPR1344] (ab204472)HRP Anti-CD11b antibody [EPR1344] (ab204472) at 1/5000 dilution + THP1 (Human acute monocytic leukemia cell line) Whole Cell Lysate + TPA at 10 µg
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 127 kDa
Observed band size: 175 kDa why is the actual band size different from the predicted?
Exposure time: 20 minutesThis blot was produced using a 3-8% Tris Acetate gel under the TA buffer system. The gel was run at 150V for 60 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab204472 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.
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![HRP Anti-CD11b antibody [EPR1344] (ab204472)](https://www.abcam.com/ps/products/204/ab204472/Images/ab204472-4-benefits-of-recombinant-antibodies.png)
HRP Anti-CD11b antibody [EPR1344] (ab204472)
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (0)
ab204472 has not yet been referenced specifically in any publications.