Recombinant HRP Anti-CD11c antibody [EP1347Y] (ab195534)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-CD11c antibody [EP1347Y] (ab195534)](https://www.abcam.com/ps/products/195/ab195534/Images/ab195534-237396-anti-cd11c-antibody-ep1347y-hrp-immunohistochemistry.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-CD11c antibody [EP1347Y] (ab195534)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- HRP Rabbit monoclonal [EP1347Y] to CD11c
- Suitable for: WB, IHC-P
- Reacts with: Human
- Conjugation: HRP
Overview
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Product name
HRP Anti-CD11c antibody [EP1347Y]
See all CD11c primary antibodies -
Description
HRP Rabbit monoclonal [EP1347Y] to CD11c -
Host species
Rabbit -
Conjugation
HRP -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human CD11c (C terminal). The exact sequence is proprietary.
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Positive control
- WB: THP-1 + TPA whole cell lysate. IHC-P: normal human tonsil tissue.
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.1% 10% Proclin 300 Solution
Constituents: 30% Glycerol, 1% BSA, PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EP1347Y -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab195534 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/5000. Detects a band of approximately 150 kDa (predicted molecular weight: 128 kDa). | |
| IHC-P | 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. ab199507 - Rabbit monoclonal IgG (HRP), is suitable for use an as isotype control with this antibody.
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Target
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Function
Integrin alpha-X/beta-2 is a receptor for fibrinogen. It recognizes the sequence G-P-R in fibrinogen. It mediates cell-cell interaction during inflammatory responses. It is especially important in monocyte adhesion and chemotaxis. -
Tissue specificity
Predominantly expressed in monocytes and granulocytes. -
Sequence similarities
Belongs to the integrin alpha chain family.
Contains 7 FG-GAP repeats.
Contains 1 VWFA domain. -
Domain
The integrin I-domain (insert) is a VWFA domain. Integrins with I-domains do not undergo protease cleavage. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 3687 Human
- Omim: 151510 Human
- SwissProt: P20702 Human
- Unigene: 248472 Human
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Alternative names
- 95 alpha chain antibody
- 95 antibody
- CD 11c antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-CD11c antibody [EP1347Y] (ab195534)
IHC image of CD11c staining in a section of formalin-fixed paraffin-embedded normal human tonsil tissue*, performed on a Leica BOND. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab195534 at 1/100 dilution, for 15 mins at room temperature. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset negative control image is taken from an identical assay without primary antibody.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
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![Western blot - HRP Anti-CD11c antibody [EP1347Y] (ab195534)](https://www.abcam.com/ps/products/195/ab195534/Images/ab195534-238102-anti-cd11c-antibody-ep1347y-hrp-western-blot.jpg)
Western blot - HRP Anti-CD11c antibody [EP1347Y] (ab195534)HRP Anti-CD11c antibody [EP1347Y] (ab195534) at 1/5000 dilution + THP1 (Human acute monocytic leukemia cell line) + TPA Whole Cell Lysate at 10 µg
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 128 kDa
Observed band size: 150 kDa why is the actual band size different from the predicted?
Exposure time: 8 secondsThis blot was produced using a 3-8% Tris Acetate gel under the TA buffer system. The gel was run at 150V for 60 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab195534 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.
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![HRP Anti-CD11c antibody [EP1347Y] (ab195534)](https://www.abcam.com/ps/products/195/ab195534/Images/ab195534-3-benefits-of-recombinant-antibodies.png)
HRP Anti-CD11c antibody [EP1347Y] (ab195534)
Protocols
References (0)
ab195534 has not yet been referenced specifically in any publications.
