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  1. Link

    hrp-cd44-antibody-epr1013y-ab194989.pdf

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Immunology Cell Type Markers CD Adhesion
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Validated using a knockout cell lineRecombinantRabMAb

Recombinant HRP Anti-CD44 antibody [EPR1013Y] (ab194989)

  • Datasheet
  • SDS
  • Certificate of Compliance
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Western blot - HRP Anti-CD44 antibody [EPR1013Y] (ab194989)
  • Western blot - HRP Anti-CD44 antibody [EPR1013Y] (ab194989)
  • HRP Anti-CD44 antibody [EPR1013Y] (ab194989)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • HRP Rabbit monoclonal [EPR1013Y] to CD44
  • Suitable for: WB
  • Knockout validated
  • Reacts with: Human
  • Conjugation: HRP

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Anti-CD44 antibody [EPR1013Y] - BSA and Azide free (ab216647)

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Overview

  • Product name

    HRP Anti-CD44 antibody [EPR1013Y]
    See all CD44 primary antibodies
  • Description

    HRP Rabbit monoclonal [EPR1013Y] to CD44
  • Host species

    Rabbit
  • Conjugation

    HRP
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
    Does not react with: Mouse, Rat
  • Immunogen

    Synthetic peptide within Human CD44 aa 150-250. The exact sequence is proprietary.

  • Positive control

    • WB: HeLa, A549 and TF-1 whole cell lysates.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark.
  • Dissociation constant (KD)

    KD = 3.76 x 10 -10 M
    Learn more about KD
  • Storage buffer

    pH: 7.40
    Preservative: 0.1% 10% Proclin 300 Solution
    Constituents: 30% Glycerol, 1% BSA, PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR1013Y
  • Isotype

    IgG
  • Research areas

    • Immunology
    • Cell Type Markers
    • CD
    • Adhesion
    • Stem Cells
    • Mesenchymal Stem Cells
    • Surface Molecules
    • Cancer
    • Tumor immunology
    • CD markers
    • Cancer
    • Tumor biomarkers
    • Other
    • Stem Cells
    • Hematopoietic Progenitors
    • Lymphoid
    • T Lymphocytic Lineage
    • Stem Cells
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    • Hematopoietic Progenitors
    • Myeloid
    • Neutrophil Lineage
    • Kits/ Lysates/ Other
    • Kits
    • ELISA Kits
    • ELISA Kits
    • CD markers ELISA kits

Associated products

  • Alternative Versions

    • Alexa Fluor® 488 Anti-CD44 antibody [EPR1013Y] (ab194987)
    • Alexa Fluor® 647 Anti-CD44 antibody [EPR1013Y] (ab194988)
    • Alexa Fluor® 594 Anti-CD44 antibody [EPR1013Y] (ab201724)
    • Anti-CD44 antibody [EPR1013Y] - BSA and Azide free (ab216647)
    • Anti-CD44 antibody [EPR1013Y] - Low endotoxin, Azide free (ab246690)
    • Anti-CD44 antibody [EPR1013Y] (ab51037)
  • Isotype control

    • HRP Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab199507)
  • Recombinant Protein

    • Recombinant human CD44 protein (Active) (ab173996)
  • Related Products

    • ECL Substrate Kit (High Sensitivity) (ab133406)

Applications

Our Abpromise guarantee covers the use of ab194989 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2500. Detects a band of approximately 82 kDa (predicted molecular weight: 82 kDa).

Target

  • Function

    Receptor for hyaluronic acid (HA). Mediates cell-cell and cell-matrix interactions through its affinity for HA, and possibly also through its affinity for other ligands such as osteopontin, collagens, and matrix metalloproteinases (MMPs). Adhesion with HA plays an important role in cell migration, tumor growth and progression. Also involved in lymphocyte activation, recirculation and homing, and in hematopoiesis. Altered expression or dysfunction causes numerous pathogenic phenotypes. Great protein heterogeneity due to numerous alternative splicing and post-translational modification events.
  • Tissue specificity

    Isoform 10 (epithelial isoform) is expressed by cells of epithelium and highly expressed by carcinomas. Expression is repressed in neuroblastoma cells.
  • Sequence similarities

    Contains 1 Link domain.
  • Domain

    The lectin-like LINK domain is responsible for hyaluronan binding.
  • Post-translational
    modifications

    Proteolytically cleaved in the extracellular matrix by specific proteinases (possibly MMPs) in several cell lines and tumors.
    N-glycosylated.
    O-glycosylated; contains more-or-less-sulfated chondroitin sulfate glycans, whose number may affect the accessibility of specific proteinases to their cleavage site(s).
    Phosphorylated; activation of PKC results in the dephosphorylation of Ser-706 (constitutive phosphorylation site), and the phosphorylation of Ser-672.
  • Cellular localization

    Membrane.
  • Target information above from: UniProt accession P16070 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 960 Human
    • Omim: 107269 Human
    • SwissProt: P16070 Human
    • Unigene: 502328 Human
    • Alternative names

      • LHR antibody
      • BA-1 antibody
      • CD 44 antibody
      • CD44 antibody
      • CD44 antigen antibody
      • CD44 molecule (Indian blood group) antibody
      • CD44 molecule antibody
      • CD44_HUMAN antibody
      • CDw44 antibody
      • CDW44 antigen antibody
      • Cell surface glycoprotein CD44 antibody
      • chondroitin sulfate proteoglycan 8 antibody
      • CSPG8 antibody
      • ECMR-III antibody
      • Epican antibody
      • Extracellular matrix receptor III antibody
      • GP90 lymphocyte homing/adhesion receptor antibody
      • HCELL antibody
      • hematopoietic cell E- and L-selectin ligand antibody
      • Heparan sulfate proteoglycan antibody
      • Hermes antigen antibody
      • homing function and Indian blood group system antibody
      • HSA antibody
      • HUTCH-I antibody
      • HUTCH1 antibody
      • HUTCHI antibody
      • Hyaluronate receptor antibody
      • IN antibody
      • INLU-related p80 Glycoprotein antibody
      • MC56 antibody
      • MDU2 antibody
      • MDU3 antibody
      • MGC10468 antibody
      • MIC4 antibody
      • MUTCH I antibody
      • MUTCH1 antibody
      • PGP-1 antibody
      • PGP-I antibody
      • PGP1 antibody
      • Phagocytic glycoprotein 1 antibody
      • Phagocytic glycoprotein I antibody
      • Soluble CD44 antibody
      see all

    Images

    • Western blot - HRP Anti-CD44 antibody [EPR1013Y] (ab194989)
      Western blot - HRP Anti-CD44 antibody [EPR1013Y] (ab194989)
      All lanes : HRP Anti-CD44 antibody [EPR1013Y] (ab194989) at 1/2500 dilution

      Lane 1 : Wild-type HeLa cell lysate
      Lane 2 : CD44 knockout HeLa cell lysate
      Lane 3 : A549 cell lysate
      Lane 4 : LnCAP cell lysate

      Lysates/proteins at 20 µg per lane.

      Performed under reducing conditions.

      Predicted band size: 82 kDa
      Observed band size: 80 kDa
      why is the actual band size different from the predicted?



      Lanes 1 - 4: Merged signal (red and green). Green - ab194989 observed at 80 kDa. Red - loading control, ab7291 (Mouse anti-Alpha Tubulin [DM1A]) observed at 55kDa.

      ab194989 was shown to react with CD44 (HRP) in wild-type HeLa cells in western blot. Loss of signal was observed when CD44 knockout sample was used. Wild-type HeLa and CD44 knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3% milk in TBS-T (0.1% Tween®) before incubation with ab194989 and ab7291 (Mouse anti-Alpha Tubulin [DM1A]) overnight at 4°C at a 1 in 2500 Dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

    • Western blot - HRP Anti-CD44 antibody [EPR1013Y] (ab194989)
      Western blot - HRP Anti-CD44 antibody [EPR1013Y] (ab194989)
      HRP Anti-CD44 antibody [EPR1013Y] (ab194989) at 1/2500 dilution + TF-1 Whole Cell Lysate at 10 µg

      Developed using the ECL technique.

      Performed under reducing conditions.

      Predicted band size: 82 kDa
      Observed band size: 82 kDa


      Exposure time: 20 minutes


      This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab194989 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.

    • HRP Anti-CD44 antibody [EPR1013Y] (ab194989)
      HRP Anti-CD44 antibody [EPR1013Y] (ab194989)

    Protocols

    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
    • SDS

    Certificate of Compliance

    To download a Certificate of Compliance, please enter your Lot number below:

  • References (0)

    Publishing research using ab194989? Please let us know so that we can cite the reference in this datasheet.

    ab194989 has not yet been referenced specifically in any publications.

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