HRP Anti-CD90 / Thy1 antibody [MRC OX-7] (ab199219)

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Western blot - HRP Anti-CD90 / Thy1 antibody [MRC OX-7] (ab199219)

    Key features and details

    • HRP Mouse monoclonal [MRC OX-7] to CD90 / Thy1
    • Suitable for: WB
    • Reacts with: Rat
    • Conjugation: HRP
    • Isotype: IgG1

    Overview

    • Product name

      HRP Anti-CD90 / Thy1 antibody [MRC OX-7]
      See all CD90 / Thy1 primary antibodies

    • Description

      HRP Mouse monoclonal [MRC OX-7] to CD90 / Thy1

    • Host species

      Mouse

    • Conjugation

      HRP

    • Tested applications

      Suitable for: WBmore details

    • Species reactivity

      Reacts with: Rat
      Predicted to work with: Mouse, Rabbit, Guinea pig

    • Immunogen

      Full length protein corresponding to Rat CD90/ Thy1.

    • Positive control

      • WB: Rat brain tissue lysate and PC12 whole cell lysate.

    • General notes

      The affinity of the Fab' of MRC OX-7 for rat Thy-1 is 3 x 109m-1 and for mouse Thy-1.1 is 3 x 108m-1.

      Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

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      In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

      We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

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    Properties

    Applications

    Our Abpromise guarantee covers the use of ab199219 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    WB 1/5000. Detects a band of approximately 35-37 kDa (predicted molecular weight: 17 kDa).

    Target

    • Function

      May play a role in cell-cell or cell-ligand interactions during synaptogenesis and other events in the brain.

    • Sequence similarities

      Contains 1 Ig-like V-type (immunoglobulin-like) domain.

    • Cellular localization

      Cell membrane.
    • Information by UniProt

    • Database links

      • Alternative names

        • CD7 antibody
        • CD90 antibody
        • CD90 antigen antibody
        • CDw90 antibody
        • FLJ33325 antibody
        • MGC128895 antibody
        • T25 antibody
        • Theta antigen antibody
        • Thy 1 antibody
        • Thy 1 cell surface antigen antibody
        • Thy 1 membrane glycoprotein antibody
        • Thy 1 T cell antigen antibody
        • Thy 1.2 antibody
        • Thy-1 antigen antibody
        • Thy-1 membrane glycoprotein antibody
        • Thy1 antibody
        • Thy1 antigen antibody
        • Thy1 T cell antigen antibody
        • Thy1.1 antibody
        • Thy1.2 antibody
        • THY1_HUMAN antibody
        • Thymus cell antigen 1, theta antibody
        see all

      Images

      • Western blot - HRP Anti-CD90 / Thy1 antibody [MRC OX-7] (ab199219)
        Western blot - HRP Anti-CD90 / Thy1 antibody [MRC OX-7] (ab199219)
        All lanes : HRP Anti-CD90 / Thy1 antibody [MRC OX-7] (ab199219) at 1/5000 dilution

        Lane 1 : Brain (Rat) Tissue Lysate
        Lane 2 : PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate

        Lysates/proteins at 10 µg per lane.

        Developed using the ECL technique.

        Performed under reducing conditions.

        Predicted band size: 17 kDa
        Observed band size: 25 kDa
        why is the actual band size different from the predicted?


        Exposure time: 30 seconds


        This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab199219 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.

      References (0)

      Publishing research using ab199219? Please let us know so that we can cite the reference in this datasheet.

      ab199219 has not yet been referenced specifically in any publications.

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