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  1. Link

    hrp-cdc25c-antibody-e302-ab205734.pdf

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Cell Biology Cell Cycle Kinases/Phosphatases Phosphatases
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Validated using a knockout cell lineRecombinantRabMAb

Recombinant HRP Anti-Cdc25C antibody [E302] (ab205734)

  • Datasheet
  • SDS
  • Certificate of Compliance
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Western blot - HRP Anti-Cdc25C antibody [E302] (ab205734)
  • HRP Anti-Cdc25C antibody [E302] (ab205734)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • HRP Rabbit monoclonal [E302] to Cdc25C
  • Suitable for: WB
  • Knockout validated
  • Reacts with: Human
  • Conjugation: HRP

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Alexa Fluor® 647 Carrier Free Unconjugated

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Human CDC25C knockout HeLa cell line (ab265189)

View more associated products

Overview

  • Product name

    HRP Anti-Cdc25C antibody [E302]
    See all Cdc25C primary antibodies
  • Description

    HRP Rabbit monoclonal [E302] to Cdc25C
  • Host species

    Rabbit
  • Conjugation

    HRP
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Rat
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HeLa and wild-type HAP1 whole cell lysates.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. Store In the Dark.
  • Storage buffer

    pH: 7.40
    Preservative: 0.1% Proclin 300 Solution
    Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    E302
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Cell Cycle
    • Kinases/Phosphatases
    • Phosphatases
    • Epigenetics and Nuclear Signaling
    • Cell cycle
    • Kinases/Phosphatases
    • Phospatases

Associated products

  • Alternative Versions

    • Alexa Fluor® 488 Anti-Cdc25C antibody [E302] (ab205425)
    • Alexa Fluor® 647 Anti-Cdc25C antibody [E302] (ab205426)
    • Anti-Cdc25C antibody [E302] - BSA and Azide free (ab232553)
    • Anti-Cdc25C antibody [E302] (ab32444)
  • Isotype control

    • HRP Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab199507)
  • Positive Controls

    • HeLa whole cell lysate (ab29545)
  • Recombinant Protein

    • Recombinant Human Cdc25C protein (ab158076)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab205734 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/2500. Detects a band of approximately 60 kDa (predicted molecular weight: 53 kDa).
Notes
WB
1/2500. Detects a band of approximately 60 kDa (predicted molecular weight: 53 kDa).

Target

  • Function

    Functions as a dosage-dependent inducer in mitotic control. It is a tyrosine protein phosphatase required for progression of the cell cycle. It directly dephosphorylates CDK1 and activate its kinase activity.
  • Sequence similarities

    Belongs to the MPI phosphatase family.
    Contains 1 rhodanese domain.
  • Developmental stage

    Expressed predominantly in G2 phase.
  • Post-translational
    modifications

    Phosphorylated by CHK1 on Ser-216. This phosphorylation creates a binding site for 14-3-3 protein and inhibits the phosphatase. Phosphorylated by PLK4.
  • Cellular localization

    Nucleus.
  • Target information above from: UniProt accession P30307 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 995 Human
    • Entrez Gene: 307511 Rat
    • Omim: 157680 Human
    • SwissProt: P30307 Human
    • Unigene: 656 Human
    • Alternative names

      • CDC 25 antibody
      • Cdc 25C antibody
      • CDC25 antibody
      • CDC25C antibody
      • Cell division cycle 25 homolog C antibody
      • Cell division cycle 25C antibody
      • Cell division cycle 25C protein antibody
      • Dual specificity phosphatase Cdc25C antibody
      • M phase inducer phosphatase 3 antibody
      • M-phase inducer phosphatase 3 antibody
      • Mitosis inducer CDC25 antibody
      • MPIP3 antibody
      • MPIP3_HUMAN antibody
      • Phosphotyrosine phosphatase antibody
      • PPP1R60 antibody
      • protein phosphatase 1, regulatory subunit 60 antibody
      see all

    Images

    • Western blot - HRP Anti-Cdc25C antibody [E302] (ab205734)
      Western blot - HRP Anti-Cdc25C antibody [E302] (ab205734)
      All lanes : HRP Anti-Cdc25C antibody [E302] (ab205734) at 1/2500 dilution

      Lane 1 : HeLa whole cell lysate (ab150035) at 10 µg
      Lane 2 : Wild-type HAP1 cell lysate at 20 µg
      Lane 3 : Cdc25C knockout HAP1 cell lysate at 20 µg

      Developed using the ECL technique.

      Performed under reducing conditions.

      Predicted band size: 53 kDa
      Observed band size: 60 kDa why is the actual band size different from the predicted?


      Exposure time: 16 minutes


      This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab205734 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.

    • HRP Anti-Cdc25C antibody [E302] (ab205734)
      HRP Anti-Cdc25C antibody [E302] (ab205734)

    Protocols

    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    Certificate of Compliance

    To download a Certificate of Compliance, please enter your Lot number below:

    References (0)

    Publishing research using ab205734? Please let us know so that we can cite the reference in this datasheet.

    ab205734 has not yet been referenced specifically in any publications.

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