Recombinant HRP Anti-Cdc25C antibody [E302] (ab205734)
![Western blot - HRP Anti-Cdc25C antibody [E302] (ab205734)](https://www.abcam.com/ps/products/205/ab205734/Images/ab205734-270792-WBab2057341.jpg "Western blot - HRP Anti-Cdc25C antibody [E302] (ab205734)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- HRP Rabbit monoclonal [E302] to Cdc25C
- Suitable for: WB
- Knockout validated
- Reacts with: Human
- Conjugation: HRP
Related conjugates and formulations
Overview
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Product name
HRP Anti-Cdc25C antibody [E302]
See all Cdc25C primary antibodies -
Description
HRP Rabbit monoclonal [E302] to Cdc25C -
Host species
Rabbit -
Conjugation
HRP -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Rat
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HeLa and wild-type HAP1 whole cell lysates.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.1% Proclin 300 Solution
Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
E302 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab205734 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/2500. Detects a band of approximately 60 kDa (predicted molecular weight: 53 kDa).
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| Notes |
|---|
|
WB
1/2500. Detects a band of approximately 60 kDa (predicted molecular weight: 53 kDa). |
Target
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Function
Functions as a dosage-dependent inducer in mitotic control. It is a tyrosine protein phosphatase required for progression of the cell cycle. It directly dephosphorylates CDK1 and activate its kinase activity. -
Sequence similarities
Belongs to the MPI phosphatase family.
Contains 1 rhodanese domain. -
Developmental stage
Expressed predominantly in G2 phase. -
Post-translational
modificationsPhosphorylated by CHK1 on Ser-216. This phosphorylation creates a binding site for 14-3-3 protein and inhibits the phosphatase. Phosphorylated by PLK4. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 995 Human
- Entrez Gene: 307511 Rat
- Omim: 157680 Human
- SwissProt: P30307 Human
- Unigene: 656 Human
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Alternative names
- CDC 25 antibody
- Cdc 25C antibody
- CDC25 antibody
see all
Images
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Western blot - HRP Anti-Cdc25C antibody [E302] (ab205734)All lanes : HRP Anti-Cdc25C antibody [E302] (ab205734) at 1/2500 dilution
Lane 1 :HeLa whole cell lysate (ab150035) at 10 µg
Lane 2 : Wild-type HAP1 cell lysate at 20 µg
Lane 3 : Cdc25C knockout HAP1 cell lysate at 20 µg
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 53 kDa
Observed band size: 60 kDa why is the actual band size different from the predicted?
Exposure time: 16 minutesThis blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab205734 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.
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![HRP Anti-Cdc25C antibody [E302] (ab205734)](https://www.abcam.com/ps/products/205/ab205734/Images/ab205734-3-benefits-of-recombinant-antibodies.png)
HRP Anti-Cdc25C antibody [E302] (ab205734)
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (0)
ab205734 has not yet been referenced specifically in any publications.