Recombinant HRP Anti-Cdc25C antibody [E302] (ab205734)
![Western blot - HRP Anti-Cdc25C antibody [E302] (ab205734)](https://www.abcam.com/ps/products/205/ab205734/Images/ab205734-270792-WBab2057341.jpg "Western blot - HRP Anti-Cdc25C antibody [E302] (ab205734)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- HRP Rabbit monoclonal [E302] to Cdc25C
- Suitable for: WB
- Knockout validated
- Reacts with: Human
- Conjugation: HRP
Overview
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Product name
HRP Anti-Cdc25C antibody [E302]
See all Cdc25C primary antibodies -
Description
HRP Rabbit monoclonal [E302] to Cdc25C -
Host species
Rabbit -
Conjugation
HRP -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Rat
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Immunogen
Synthetic peptide within Human Cdc25C (N terminal). The exact sequence is proprietary.
Database link: P30307 -
Positive control
- WB: HeLa and wild-type HAP1 whole cell lysates.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.1% 10% Proclin 300 Solution
Constituents: PBS, 30% Glycerol, 1% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
E302 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab205734 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/2500. Detects a band of approximately 60 kDa (predicted molecular weight: 53 kDa). |
Target
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Function
Functions as a dosage-dependent inducer in mitotic control. It is a tyrosine protein phosphatase required for progression of the cell cycle. It directly dephosphorylates CDK1 and activate its kinase activity. -
Sequence similarities
Belongs to the MPI phosphatase family.
Contains 1 rhodanese domain. -
Developmental stage
Expressed predominantly in G2 phase. -
Post-translational
modificationsPhosphorylated by CHK1 on Ser-216. This phosphorylation creates a binding site for 14-3-3 protein and inhibits the phosphatase. Phosphorylated by PLK4. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 995 Human
- Entrez Gene: 307511 Rat
- Omim: 157680 Human
- SwissProt: P30307 Human
- Unigene: 656 Human
- Unigene: 162298 Rat
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Alternative names
- CDC 25 antibody
- Cdc 25C antibody
- CDC25 antibody
see all
Images
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Western blot - HRP Anti-Cdc25C antibody [E302] (ab205734)All lanes : HRP Anti-Cdc25C antibody [E302] (ab205734) at 1/2500 dilution
Lane 1 :HeLa whole cell lysate (ab150035) at 10 µg
Lane 2 : Wild-type HAP1 cell lysate at 20 µg
Lane 3 : Cdc25C knockout HAP1 cell lysate at 20 µg
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 53 kDa
Observed band size: 60 kDa why is the actual band size different from the predicted?
Exposure time: 16 minutesThis blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab205734 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.
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![HRP Anti-Cdc25C antibody [E302] (ab205734)](https://www.abcam.com/ps/products/205/ab205734/Images/ab205734-3-benefits-of-recombinant-antibodies.png)
HRP Anti-Cdc25C antibody [E302] (ab205734)
References (0)
ab205734 has not yet been referenced specifically in any publications.
