Recombinant HRP Anti-CDKN2A/p16INK4a antibody [EPR1473] (ab192080)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- HRP Rabbit monoclonal [EPR1473] to CDKN2A/p16INK4a
- Suitable for: WB, IHC-P
- Reacts with: Human
- Conjugation: HRP
Overview
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Product name
HRP Anti-CDKN2A/p16INK4a antibody [EPR1473]
See all CDKN2A/p16INK4a primary antibodies -
Description
HRP Rabbit monoclonal [EPR1473] to CDKN2A/p16INK4a -
Host species
Rabbit -
Conjugation
HRP -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human CDKN2A/p16INK4a aa 100 to the C-terminus (C terminal). The exact sequence is proprietary.
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Positive control
- WB: HeLa, HEK293. IHC. Human lung - Squamous cell carcinoma tissue
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.1% 10% Proclin 300 Solution
Constituents: 30% Glycerol, 1% BSA, PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR1473 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
- Anti-CDKN2A/p16INK4a antibody [EPR1473] - C-terminal (ab108349)
- Anti-CDKN2A/p16INK4a antibody [EPR1473] - BSA and Azide free (ab186932)
- Alexa Fluor® 488 Anti-CDKN2A/p16INK4a antibody [EPR1473] (ab192053)
- Alexa Fluor® 647 Anti-CDKN2A/p16INK4a antibody [EPR1473] (ab192054)
- PE Anti-CDKN2A/p16INK4a antibody [EPR1473] (ab209579)
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
Our Abpromise guarantee covers the use of ab192080 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | 1/1000. Detects a band of approximately 17 kDa (predicted molecular weight: 17 kDa). | |
IHC-P | 1/100 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
Target
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Cellular localization
Cytoplasmic and Nuclear -
Database links
- Entrez Gene: 1029 Human
- Omim: 600160 Human
- SwissProt: P42771 Human
- Unigene: 512599 Human
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Form
There are 4 isoforms produced by alternative splicing. Isoform 1 also known as: p16INK4a; Isoform 3 also known as: p12; Isoform 4 also known as: p14ARF; p19ARF; ARF. -
Alternative names
- CCM2 antibody
- CDK4 inhibitor p16 INK4 antibody
- CDK4I antibody
see all
Images
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All lanes : HRP Anti-CDKN2A/p16INK4a antibody [EPR1473] (ab192080) at 1/1000 dilution
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 17 kDa
Observed band size: 17 kDa
Exposure time: 20 minutesThis blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab192080 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-CDKN2A/p16INK4a antibody [EPR1473] (ab192080)
IHC image of CDKN2A/p16INK4a staining in a section of formalin-fixed paraffin-embedded Human lung squamous cell carcinoma. The section was pre-treated using pressure cooker heat mediated antigen retrieval with sodium citrate buffer (pH6) for 30 mins. The section was incubated with ab192080 at a working dilution of 1 in 100 overnight at +4°C. The section was counterstained with haematoxylin and mounted with DPX.
This image shows an area of stained macrophage cells. The inset negative control image is taken from an identical assay without primary antibody.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-CDKN2A/p16INK4a antibody [EPR1473] (ab192080)
IHC image of CDKN2A/p16INK4a staining in a section of formalin-fixed paraffin-embedded Human lung squamous cell carcinoma. The section was pre-treated using pressure cooker heat mediated antigen retrieval with sodium citrate buffer (pH6) for 30 mins. The section was incubated with ab192080 at a working diltuion of 1 in 100 overnight at +4°C. The section was counterstained with haematoxylin and mounted with DPX.
This image shows an area of cancerous tissue. The inset negative control image is taken from an identical assay without primary antibody.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Protocols
References (0)
ab192080 has not yet been referenced specifically in any publications.