Recombinant HRP Anti-CDKN2A/p16INK4a antibody [EPR1473] (ab192080)

This blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab192080 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.

IHC image of CDKN2A/p16INK4a staining in a section of formalin-fixed paraffin-embedded Human lung squamous cell carcinoma. The section was pre-treated using pressure cooker heat mediated antigen retrieval with sodium citrate buffer (pH6) for 30 mins. The section was incubated with ab192080 at a working dilution of 1 in 100 overnight at +4°C. The section was counterstained with haematoxylin and mounted with DPX.

This image shows an area of stained macrophage cells. The inset negative control image is taken from an identical assay without primary antibody.

For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

IHC image of CDKN2A/p16INK4a staining in a section of formalin-fixed paraffin-embedded Human lung squamous cell carcinoma. The section was pre-treated using pressure cooker heat mediated antigen retrieval with sodium citrate buffer (pH6) for 30 mins. The section was incubated with ab192080 at a working diltuion of 1 in 100 overnight at +4°C. The section was counterstained with haematoxylin and mounted with DPX.

This image shows an area of cancerous tissue. The inset negative control image is taken from an identical assay without primary antibody.

For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.