Key features and details
- HRP Rat monoclonal [5-C3] to CDKN2A/p19ARF
- Suitable for: WB
- Reacts with: Mouse
- Conjugation: HRP
- Isotype: IgG2b
Product nameHRP Anti-CDKN2A/p19ARF antibody [5-C3]
See all CDKN2A/p19ARF primary antibodies
DescriptionHRP Rat monoclonal [5-C3] to CDKN2A/p19ARF
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Mouse
Does not react with: Hamster, Human
Synthetic peptide within Mouse CDKN2A/p19ARF aa 50-150 conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.
- WB: MEF-1 whole cell lystate.
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark.
Storage bufferpH: 7.40
Preservative: 0.1% 10% Proclin 300 Solution
Constituents: PBS, 30% Glycerol, 1% BSA
Concentration information loading...
Our Abpromise guarantee covers the use of ab202225 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/5000. Detects a band of approximately 19 kDa (predicted molecular weight: 17 kDa).|
RelevanceThe gene for CDK2NA generates several transcripts/proteins which differ from each other in their first exons. Three of these transcripts are generated by alternative splicing (isoform 1 a.k.a p16INK4A, isoform 2 and isoform 3 a.k.a p12), two of which are known to function as inhibitors of CDK4 kinase. One other transcript that is generated from this gene contains an alternate reading frame (ARF), with the first exon located 20kb upstream of the remainder of the gene(isoform 4 a.k.a. p14ARF, p19ARF, ARF). In spite of the structural and some functional differences, all the proteins encoded by the CDKN2A gene are involved in cell cycle G1 control.
- ARF antibody
- CDKN2A antibody
- Cyclin dependent kinase inhibitor 2A antibody
HRP Anti-CDKN2A/p19ARF antibody [5-C3] (ab202225) at 1/5000 dilution + MEF1 (Mouse embryonic fibroblast cell line) Whole Cell Lysate at 10 µg
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 17 kDa
Observed band size: 19 kDa why is the actual band size different from the predicted?
Exposure time: 8 minutes
This blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab202225 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.
ab202225 has been referenced in 2 publications.
- Miao J et al. Wnt/ß-catenin/RAS signaling mediates age-related renal fibrosis and is associated with mitochondrial dysfunction. Aging Cell 18:e13004 (2019). PubMed: 31318148
- Luo C et al. Wnt9a Promotes Renal Fibrosis by Accelerating Cellular Senescence in Tubular Epithelial Cells. J Am Soc Nephrol 29:1238-1256 (2018). PubMed: 29440280