Recombinant HRP Anti-CLCA1 antibody [EPR12254-88] (ab203736)
![Western blot - HRP Anti-CLCA1 antibody [EPR12254-88] (ab203736)](https://www.abcam.com/ps/products/203/ab203736/Images/ab203736-265668-WBab2037361.jpg "Western blot - HRP Anti-CLCA1 antibody [EPR12254-88] (ab203736)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- HRP Rabbit monoclonal [EPR12254-88] to CLCA1
- Suitable for: WB
- Reacts with: Human
- Conjugation: HRP
Overview
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Product name
HRP Anti-CLCA1 antibody [EPR12254-88]
See all CLCA1 primary antibodies -
Description
HRP Rabbit monoclonal [EPR12254-88] to CLCA1 -
Host species
Rabbit -
Conjugation
HRP -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat
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Immunogen
Recombinant fragment within Human CLCA1. The exact sequence is proprietary.
Database link: A8K7I4 -
Positive control
- WB: Small Intestine (Human) Tissue Lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.1% 10% Proclin 300 Solution
Constituents: PBS, 30% Glycerol, 1% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR12254-88 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab203736 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000. Detects a band of approximately 85 kDa (predicted molecular weight: 100 kDa). |
Target
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Function
May be involved in mediating calcium-activated chloride conductance. May play critical roles in goblet cell metaplasia, mucus hypersecretion, cystic fibrosis and AHR. May be involved in the regulation of mucus production and/or secretion by goblet cells. Involved in the regulation of tissue inflammation in the innate immune response. May play a role as a tumor suppressor. Induces MUC5AC. -
Tissue specificity
Highly expressed in small intestine and colon namely in intestinal basal crypt epithelia and goblet cells, and appendix. Weakly expressed in uterus, testis and kidney. Expressed in the airways epithelium of both asthmatic andhealthy patients. Expressed in the bronchial epithelium, especially in mucus-producing goblet cells. Expressed in normal turbinate mucosa and nasal polyp. Expressed in. -
Sequence similarities
Belongs to the CLCR family.
Contains 1 VWFA domain. -
Post-translational
modificationsGlycosylated.
The 125-kDa product is processed and yields to two cell-surface-associated subunits, a 90-kDa protein and a group of 37-to 41-kDa proteins. -
Cellular localization
Secreted > extracellular space. Cell membrane. Protein that remains attached to the plasma membrane appeared to be predominantly localized to microvilli. - Information by UniProt
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Database links
- Entrez Gene: 1179 Human
- Entrez Gene: 23844 Mouse
- Entrez Gene: 308015 Rat
- Omim: 603906 Human
- SwissProt: A8K7I4 Human
- SwissProt: Q9D7Z6 Mouse
- Unigene: 194659 Human
- Unigene: 33483 Mouse
see all -
Alternative names
- CaCC antibody
- CaCC-1 antibody
- CACC1 antibody
see all
Images
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Western blot - HRP Anti-CLCA1 antibody [EPR12254-88] (ab203736)HRP Anti-CLCA1 antibody [EPR12254-88] (ab203736) at 1/1000 dilution + Small Intestine (Human) Tissue Lysate at 10 µg
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 100 kDa
Observed band size: 85 kDa why is the actual band size different from the predicted?
Exposure time: 16 minutesThis blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab203736 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.
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![HRP Anti-CLCA1 antibody [EPR12254-88] (ab203736)](https://www.abcam.com/ps/products/203/ab203736/Images/ab203736-3-benefits-of-recombinant-antibodies.png)
HRP Anti-CLCA1 antibody [EPR12254-88] (ab203736)
References (0)
ab203736 has not yet been referenced specifically in any publications.
