Validated using a knockout cell line

HRP Anti-CNPase antibody [11-5B] (ab201678)

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Western blot - HRP Anti-CNPase antibody [11-5B] (ab201678)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-CNPase antibody [11-5B] (ab201678)
  • Western blot - HRP Anti-CNPase antibody [11-5B] (ab201678)

Key features and details

  • HRP Mouse monoclonal [11-5B] to CNPase
  • Suitable for: WB, IHC-P
  • Knockout validated
  • Reacts with: Mouse, Rat, Human
  • Conjugation: HRP
  • Isotype: IgG1

Overview

  • Product name

    HRP Anti-CNPase antibody [11-5B]
    See all CNPase primary antibodies

  • Description

    HRP Mouse monoclonal [11-5B] to CNPase

  • Host species

    Mouse

  • Conjugation

    HRP

  • Tested applications

    Suitable for: WB, IHC-Pmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human
    Predicted to work with: Sheep, Rabbit, Cow, Dog, Pig, Rhesus monkeyDoes not react with: Chicken, Guinea pig

  • Immunogen

    Full length native protein (purified) corresponding to Human CNPase.

  • Positive control

    • WB: Human, Mouse and Rat Spinal Cord and Brain tissue lysates. IHC-P: FFPE human normal cerebral cortex tissue sections.

  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Applications

Our Abpromise guarantee covers the use of ab201678 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/5000. Detects a band of approximately 48 kDa (predicted molecular weight: 48 kDa).
IHC-P 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

Images

  • Western blot - HRP Anti-CNPase antibody [11-5B] (ab201678)
    Western blot - HRP Anti-CNPase antibody [11-5B] (ab201678)
    All lanes : HRP Anti-CNPase antibody [11-5B] (ab201678) at 1/5000 dilution

    Lane 1 : Wild-type HAP1 whole cell lysate
    Lane 2 : CNPase knockout HAP1 whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Predicted band size: 48 kDa
    Observed band size: 48 kDa


    Exposure time: 20 minutes


    ab201678 was shown to specifically react with CNPase in wild-type HAP1 cells as signal was lost in CNPase knockout cells. Wild-type and CNPase knockout samples were subjected to SDS-PAGE. Ab201678 and ab184095 (Mouse monoclonal [mAbcam 9484] to GAPDH - Loading Control (Alexa Fluor® 680) loading control) were incubated overnight at 4°C at 1/5000 dilution and 1/1000 dilution respectively. The loading control was imaged using the Licor Odyssey CLx prior to blots being developed with ECL technique.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-CNPase antibody [11-5B] (ab201678)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-CNPase antibody [11-5B] (ab201678)

    IHC image of CNPase staining in a section of formalin-fixed paraffin-embedded human normal cerebral cortex*. The section was pre-treated using pressure cooker heat mediated antigen retrieval with sodium citrate buffer (pH6) for 30mins, and incubated overnight at +4°C with ab201678 at 1/100 dilution. DAB was used as the chromogen (ab103723), diluted 1/100 and incubated for 10min at room temperature. The section was counterstained with haematoxylin and mounted with DPX. The inset negative control image is taken from an identical assay without primary antibody.

     For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • Western blot - HRP Anti-CNPase antibody [11-5B] (ab201678)
    Western blot - HRP Anti-CNPase antibody [11-5B] (ab201678)
    All lanes : HRP Anti-CNPase antibody [11-5B] (ab201678) at 1/5000 dilution

    Lane 1 : Brain (Human) Tissue Lysate - adult normal tissue
    Lane 2 : Spinal Cord (Human) Tissue Lysate - adult normal tissue
    Lane 3 : Brain (Mouse) Tissue Lysate
    Lane 4 : Spinal Cord (Mouse) Tissue Lysate
    Lane 5 : Brain (Rat) Tissue Lysate
    Lane 6 : Spinal Cord (Rat) Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 48 kDa
    Observed band size: 48 kDa


    Exposure time: 6 seconds


    This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab201678 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.

     

References (0)

Publishing research using ab201678? Please let us know so that we can cite the reference in this datasheet.

ab201678 has not yet been referenced specifically in any publications.

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