Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- HRP Rabbit monoclonal [EPR17072] to Collagen VI
- Suitable for: WB
- Reacts with: Human
- Conjugation: HRP
Product nameHRP Anti-Collagen VI antibody [EPR17072]
See all Collagen VI primary antibodies
DescriptionHRP Rabbit monoclonal [EPR17072] to Collagen VI
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
Recombinant fragment within Human Collagen VI aa 1-250. The exact sequence is proprietary.
Database link: P12109
- WB: Human skeletal muscle lysate and WI-38 whole cell lysates.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark.
Storage bufferpH: 7.40
Preservative: 0.1% 10% Proclin 300 Solution
Constituents: PBS, 30% Glycerol, 1% BSA
Concentration information loading...
PurityProtein A purified
- Anti-Collagen VI antibody [EPR17072] (ab182744)
- Alexa Fluor® 488 Anti-Collagen VI antibody [EPR17072] (ab200429)
- Alexa Fluor® 647 Anti-Collagen VI antibody [EPR17072] (ab200430)
- Alexa Fluor® 594 Anti-Collagen VI antibody [EPR17072] (ab207292)
- Anti-Collagen VI antibody [EPR17072] - Low endotoxin, Azide free (ab229450)
Our Abpromise guarantee covers the use of ab200596 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/5000. Detects a band of approximately 147 kDa (predicted molecular weight: 109 kDa).|
FunctionCollagen VI acts as a cell-binding protein.
Involvement in diseaseDefects in COL6A1 are a cause of Bethlem myopathy (BM) [MIM:158810]. BM is a rare autosomal dominant proximal myopathy characterized by early childhood onset (complete penetrance by the age of 5) and joint contractures most frequently affecting the elbows and ankles.
Defects in COL6A1 are a cause of Ullrich congenital muscular dystrophy (UCMD) [MIM:254090]; also known as Ullrich scleroatonic muscular dystrophy. UCMD is an autosomal recessive congenital myopathy characterized by muscle weakness and multiple joint contractures, generally noted at birth or early infancy. The clinical course is more severe than in Bethlem myopathy.
Sequence similaritiesBelongs to the type VI collagen family.
Contains 3 VWFA domains.
modificationsProlines at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains.
Cellular localizationSecreted > extracellular space > extracellular matrix.
- Information by UniProt
- Alpha 1 (VI) chain (61 AA) antibody
- CO6A1_HUMAN antibody
- COL6A1 antibody
All lanes : HRP Anti-Collagen VI antibody [EPR17072] (ab200596) at 1/5000 dilution
Lane 1 : Skeletal Muscle (Human) Tissue Lysate - adult normal tissue at 20 µg
Lane 2 : WI38 (Human lung fibroblast cell line) Whole Cell Lysate at 10 µg
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 109 kDa
Observed band size: 147 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutes
This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab200596 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.
ab200596 has not yet been referenced specifically in any publications.