Overview

  • Product name
    HRP Conjugation Kit
  • Product overview

    HRP Conjugation Kit ab102890 uses a simple and quick process to conjugate an antibody to HRP. It can also be used to conjugate other proteins or peptides.


    To conjugate an antibody to HRP using this kit:
    - add modifier to antibody and incubate for 3 hrs
    - add quencher and incubate for 30 mins
    The conjugated antibody can be used immediately in WB, ELISA, IHC etc. No further purification is required and 100% of the antibody is recovered for use.


    Learn about buffer compatibility below; for incompatible buffers and low antibody concentrations, use our rapid antibody purification and concentration kits. Use the FAQ to learn more about the technology, or about conjugating other proteins and peptides to HRP.

  • Notes

    Amount and volume of antibody for conjugation to HRP

     Kit size   Recommended
    amount of antibody
    1 
    Maximum
    amount of antibody
    Maximum antibody
    volume2
    30 µg   3 x 10 µg  3 x 40 µg 3 x 10 µL
    100 µg   100 µg   400 µg 100 µL
    300 µg   3 x 100 µg  3 x 400 µg 3 x 100 µL
    1 mg   1 mg 4 mg 1 mL

    1 Recommended amount of antibody will give an HRP:antibody molar ratio of 4:1. Antibodies often also perform well at the maximum amount of antibody which is 1:1 molar ratio.

    2 Ideal antibody concentration is 1mg/ml. 0.5 - 1 mg/ml can be used if the maximum antibody volume is not exceeded. Antibodies > 5mg/ml or < 0.5 mg/ml should be diluted /concentrated.

     

    Buffer Requirements for Conjugation

    Buffer should be pH 6.5-8.5.

    Compatible buffer constituents
    If a concentration is shown, then the constituent should be no more than the concentration shown. If several constituents are close to the limit of acceptable concentration, then this can inhibit conjugation.

    50mM / 0.6% Tris1 0.1%/1% BSA2 50% glycerol
    0.1% sodium azide3 PBS Potassium phosphate
    Sodium chloride HEPES Sucrose
    Sodium citrate EDTA Trehalose

    1 Tris buffered saline is almost always ≤ 50 mM / 0.6%
    2 1% BSA gives lower quality conjugates, BSA can also interfere with the use of the conjugated antibody in tissue staining.
    3 Sodium azide irreversibly inhibits HRP; antibodies with azide should be purified before using the HRP conjugation kit.

    Incompatible buffer constituents

    Thiomerosal Proclin Glycine
    Arginine Glutathione DTT

    If a constituent of the buffer containing your antibody or protein is not listed above, please check the FAQ or contact us.

    Only purified antibodies are suitable for use, ie. where other proteins, peptides, or amino acids are not present: antibodies in ascites fluid, serum or hybridoma culture media are incompatible.

  • Tested applications
    Suitable for: Conjugationmore details

Properties

Applications

Our Abpromise guarantee covers the use of ab102890 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Conjugation Use at an assay dependent dilution.

Images

Protocols

References

This product has been referenced in:
  • Park JK  et al. Evaluation of Preexisting Anti-Hemagglutinin Stalk Antibody as a Correlate of Protection in a Healthy Volunteer Challenge with Influenza A/H1N1pdm Virus. MBio 9:N/A (2018). Read more (PubMed: 29362240) »
  • Joe MK  et al. Myocilin Regulates Metalloprotease 2 Activity Through Interaction With TIMP3. Invest Ophthalmol Vis Sci 58:5308-5318 (2017). Read more (PubMed: 29049729) »

See all 5 Publications for this product

Customer reviews and Q&As

Thank you for contacting us.
As my colleague mentioned, we do not currently carry any antibodies to the Aftose virus. You may be able to find one by searching biocompare.com.
For the other products you mentioned, we do have a number of HRP co...

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Thank you for your inquiry. In case the customer would like to perform a sandwich ELISA (sELISA) for the EBV viral capsid antigen, I would suggest to test the antibody clones [2E08] and [8.F.90] (ab48414 and ab32803, see below). To our knowledge, t...

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Thank you for contacting us. I am sorry to hear you are experiencing difficulties with some of our products. We take product complaints very seriously, and investigate every product that we feel may not be performing correctly. In this case the...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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