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    hrp-conjugation-kit-lightning-link-ab102890.pdf

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HRP Conjugation Kit - Lightning-Link® (ab102890)

  • Datasheet
  • SDS
Reviews (2)Q&A (3)References (133)

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Conjugation - HRP Conjugation Kit - Lightning-Link® (ab102890)
  • Conjugation - HRP Conjugation Kit - Lightning-Link® (ab102890)
  • Western blot - HRP Conjugation Kit- Lightning-Link(ab102890)
  • ELISA - HRP Conjugation Kit Lightning-Link
  • HRP Conjugation Kit
  • HRP Conjugation Kit - Lightning-Link® labeling monoclonal and polyclonal rabbit IgG for ELISA
  • HRP Conjugation Kit - Lightning-Link® labeling C3C antibody for competitive ELISA
  • HRP Conjugation Kit - Lightning-Link® labeling polyclonal goat and Mab-16E3 anti-MPO antibodies for ELISA
  • HRP Conjugation Kit - Lightning-Link® labeling monoclonal EL-NE antibody for competitive ELISA
  • HRP conjugation kit ab102890 was used to develop Frataxin ELISA kit ab176112

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Overview

  • Product name

    HRP Conjugation Kit - Lightning-Link®
  • Product overview

    HRP Conjugation Kit / HRP Labeling Kit ab102890 uses a simple and quick process for HRP labeling / conjugation of antibodies. It can also be used to conjugate other proteins or peptides. Learn about our antibody labeling kits and their advantages.


    To conjugate an antibody to HRP using this kit:
    - add modifier to antibody and incubate for 3 hrs
    - add quencher and incubate for 30 mins
    The HRP conjugated antibody can be used immediately in WB, ELISA, IHC etc. No further purification is required and 100% of the antibody is recovered for use.


    Learn about buffer compatibility below; for incompatible buffers and low antibody concentrations, use our rapid antibody purification and concentration kits. Use the FAQ to learn more about the technology, or about conjugating other proteins and peptides to HRP.


    Custom size conjugation kits up to 100 mg are available on demand. Please contact us to discuss your requirements.

  • Notes

    This product is manufactured by Expedeon, an Abcam company, and was previously called Lightning-Link® HRP Labeling Kit. 701-0004 is the same as the 5 mg size. 701-0003 is the same as the 5 x 1 mg size. 701-0000 is the same as the 3 x 100 ug size. 701-0030 is the same as the 3 x 10 ug size. 701-0010 is the same as the 100 µg size. 701-0002 is the same as the 1 mg size.

    Amount and volume of antibody for conjugation to HRP

     Kit size Recommended
    amount of antibody
    1 
    Maximum
    amount of antibody
    Maximum antibody
    volume2
    3 x 10 µg 3 x 10 µg 3 x 40 µg 3 x 10 µL
    100 µg 1 x 100 µg  1 x 400 µg 1 x 100 µL
    3 x 100 µg 3 x 100 µg  3 x 400 µg 3 x 100 µL
    1 mg 1 x 1 mg 1 x 4 mg 1 x 1 mL
    5 x 1 mg 5 x 1 mg 5 x 4 mg 5 x 1 mL
    5 mg 1 x 5 mg 1 x 20 mg 1 x 5 mL

    1 Recommended amount of antibody will give an HRP:antibody molar ratio of 4:1. Antibodies often also perform well at the maximum amount of antibody which is 1:1 molar ratio.

    2 Ideal antibody concentration is 1mg/ml. 0.5 - 1 mg/ml can be used if the maximum antibody volume is not exceeded. Antibodies > 4 mg/ml or < 0.5 mg/ml should be diluted /concentrated.

     

    Buffer Requirements for Conjugation

    Buffer should be pH 6.5-8.5.

    Buffer constituent compatibility
    If a concentration is shown, then the constituent should be no more than the concentration shown. If several constituents are close to the limit of acceptable concentration, then this can inhibit conjugation.

    Compatible buffer constituents
    50mM / 0.6% Tris1 0.1% BSA 50% glycerol
    0.1% sodium azide2 PBS Potassium phosphate
    Sodium chloride HEPES Sucrose
    Sodium citrate EDTA Trehalose

    1 Tris buffered saline is almost always ≤ 50 mM / 0.6%
    2 Sodium azide irreversibly inhibits HRP; antibodies with azide should be purified before using the HRP conjugation kit.

     

    Incompatible buffer constituents
    Thiomerosal Proclin Glycine
    Arginine Glutathione DTT

    If a constituent of the buffer containing your antibody or protein is not listed above, please check the FAQ or contact us.

    Only purified antibodies are suitable for use, ie. where other proteins, peptides, or amino acids are not present: antibodies in ascites fluid, serum or hybridoma culture media are incompatible.

     

    Storing and handling conjugation kits

    Lyophilized Lightning-Link® components are hygroscopic.

    Kits are intentionally shipped at ambient temperature with silica gel to avoid exposure to moisture. Upon receipt, store the kit frozen and protect from moisture. Before opening the outer container, allow the lyophilized components to reach room temperature to minimize condensation.

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components 100 µg 1 mg 3 x 10 µg 5 x 1 mg 3 x 100 µg 1 x 5 mg
    ab274112 - HRP mix 1 x 100µg 1 x 1mg 3 x 10µg 5 x 1mg 3 x 100µg 1 x 5mg
    ab274106 - Modifier reagent 1 x 200µl 1 x 200µl 1 x 200µl 1 x 1200µl 1 x 200µl 1 x 1200µl
    ab274296 - Quencher reagent 1 x 200µl 1 x 200µl 1 x 200µl 1 x 1200µl 1 x 200µl 1 x 1200µl
  • Research areas

    • Tags & Cell Markers
    • Epitope Tags
    • Conjugates
    • Kits/ Lysates/ Other
    • Kits
    • Conjugation Kits
    • HRP
  • Alternative names

    • Horseradish peroxidase

Images

  • Conjugation - HRP Conjugation Kit&nbsp;- Lightning-Link&reg; (ab102890)
    Conjugation - HRP Conjugation Kit - Lightning-Link® (ab102890)Image from Parkash, Om, et al., Diagnostics (Basel), 11(1):33; doi: 10.3390/diagnostics11010033. Reproduced under the Creative Commons license https://creativecommons.org/licenses/by/4.0/

    Parkash, Om, et al used HRP Conjugation Kit - Lightning-Link® (ab102890) as part of the development and evaluation of a biosensor based on screen-printed carbon electrodes (SPCEs) for the detection of dengue-specific immunoglobulin M (IgM) antibodies. They used the kit to conjugate HRP to anti-dengue antibody for use in conjugation.
    (A) Schematic diagram of the screen printed carbon electrode (SCPE)-based dengue IgM biosensor. The biosensor was constructed by sequentially adding optimised concentration of anti-human IgM capture antibody, blocking agent, human IgM antibody or serum sample, dengue antigen and detection antibody, with washing steps in between. Electrochemical signal was generated following addition of TMB substrate. (B) Comparison of various immobilisation techniques for the goat anti-human IgM capture antibody. NC: negative control consisting of a dengue IgM negative serum sample; PC: dengue IgM positive serum sample. (C) Field Emission Scanning Electron Microscopy (FESEM) surface images of (left panel) a bare carbon electrode and (right panel) a carbon electrode modified with an anti-human IgM antibody using the streptavidin/biotin immobilisation system. Both capture and detection antibodies were labeled using Lightning-Link® conjugation kits (ab201796 and ab102890).

  • Conjugation - HRP Conjugation Kit&nbsp;- Lightning-Link&reg; (ab102890)
    Conjugation - HRP Conjugation Kit - Lightning-Link® (ab102890)Image from Rackus et al., Lab Chip., 17(13):2272-2280; doi: 10.1039/c7lc00440k.Reproduced under the Creative Commons license https://creativecommons.org/licenses/by/3.0/

    Rackus, Darius G., et al used HRP Conjugation Kit - Lightning-Link® (ab102890) as part of examining digital microfluidics for detection a malaria biomarker. They used the kit to conjugate HRP to anti-Plasmodium falciparum LDH antibody for use in pre-concentration by liquid intake by paper (P-CLIP).
    DMF immunoassay for PfLDH. Comparison of mean signals obtained by traditional DMF-ELISA (black) and P-CLIP modified DMF-ELISA (grey) for concentrations below the limit of detection (7 ng mL-1) and limit of quantitation (70 ng mL-1) of the traditional DMF-ELISA. Error bars ± 1 std. dev. (n = 3).

  • Western blot - HRP Conjugation Kit- Lightning-Link(ab102890)
    Western blot - HRP Conjugation Kit- Lightning-Link(ab102890)Image from Lemaire, Katleen, et al., PloS one, 6(4): e18517; doi: 10.1371/journal.pone.0018517. Reproduced under the Creative Commons license https://creativecommons.org/licenses/by/4.0/

    Lemaire, Katleen, et al used HRP Conjugation Kit - Lightning-Link® (ab102890) as part of examining protein interactions. They used the kit to conjugate HRP to anti-BIP and anti-UFBP1 antibodies for use in detection by western blot after immunoprecipitation.
    Co-immunoprecipitation with BiP, UFL1 and UFM1 specific antibodies. BiP and UFBP1 antibodies were conjugated to HRP using ab102890 for detection after immunoprecipitation.

  • ELISA - HRP Conjugation Kit Lightning-Link
    ELISA - HRP Conjugation Kit Lightning-LinkImage from Cervin, Jakob, et al., PLoS pathogens?14.2 (2018): e1006862. Reproduced under the Creative Commons license https://creativecommons.org/licenses/by/4.0/

    Cervin, Jakob, et al used HRP Conjugation Kit - Lightning-Link® (ab102890) as part of examining binding of Cholera Toxin subunit B (CTB) to blood group antigen LewisX (LeX). They used the kit to conjugate HRP to CTB and G33D (mutated version of CTB) for use in ELISA.
    ELISA with titrated amounts human serum albumin-linked oligosaccharides (HSA-OS), immobilized to wells and detected with (B) CTB-HRP and (C) G33D-HRP. Graph shows absorbance values from three independent experiments.

  • HRP Conjugation Kit
    HRP Conjugation Kit
  • HRP Conjugation Kit - Lightning-Link® labeling monoclonal and polyclonal rabbit IgG for ELISA
    HRP Conjugation Kit - Lightning-Link® labeling monoclonal and polyclonal rabbit IgG for ELISAImage from Gram M et al.,PLoS One, 10(9):e0138111. Fig 1.; doi: 10.1371/journal.pone.0138111. Reproduced under the Creative Commons license https://creativecommons.org/licenses/by/4.0/

    Gram  M et al. used ab102890 to assess levels of cell free fetal haemoglobin (HbF) and haptoglobin (Hp) by ELISA.

    Samples were from normal pregnancies (Control) and women diagnosed with PE. The cell-free HbF plasma concentration of each patient sample (Control and PE) was plotted against the Hp plasma concentration.

  • HRP Conjugation Kit - Lightning-Link® labeling C3C antibody for competitive ELISA
    HRP Conjugation Kit - Lightning-Link® labeling C3C antibody for competitive ELISAImage from Rasmussen DG et al., PLoS One, 12(1):e0170023. Fig 2.; doi: 10.1371/journal.pone.0170023. Reproduced under the Creative Commons license https://creativecommons.org/licenses/by/4.0/

    Rasmussen DG et al. used ab102890 to determine levels of C3C by competitive ELISA.

    (A) Standard curve and inhibition of the competitive ELISA signal using healthy human serum, (B) human heparin plasma and (C) human EDTA plasma. The native material was run from undiluted and up to 8-fold diluted as indicated. (D) Neo-epitope specificity of the C3C ELISA was shown by comparing reactivity towards an elongated peptide, i.e. a peptide with an additional amino acid at the N-terminal generated by cleavage, a non-sense peptide, i.e. a peptide with a different sequence, and the standard peptide. The standard peptide (i.e. standard curve), elongated peptide, and non-sense peptide were diluted 2-fold from 100 ng/mL. The data is presented as percent (%) of background absorbance, which is the absorbance with only assay buffer present, as a function of peptide concentration.

  • HRP Conjugation Kit - Lightning-Link® labeling polyclonal goat and Mab-16E3 anti-MPO antibodies for ELISA
    HRP Conjugation Kit - Lightning-Link® labeling polyclonal goat and Mab-16E3 anti-MPO antibodies for ELISAImage from Laura RP et al., PloS One, 11(2):e0149391. Fig 2.; doi: 10.1371/journal.pone.0149391. Reproduced under the Creative Commons license https://creativecommons.org/licenses/by/4.0/

    Laura RP et al. used ab102890 to determine concentrations of MPO by ELISA.

    The relative amounts of secreted versus cellular myeloperoxidase (MPO) were calculated for each cell line.

  • HRP Conjugation Kit - Lightning-Link® labeling monoclonal EL-NE antibody for competitive ELISA
    HRP Conjugation Kit - Lightning-Link® labeling monoclonal EL-NE antibody for competitive ELISAImage from Kristensen JH et al., BMC Pulm Med., 15:53. Fig 3.; doi: 10.1186/s12890-015-0048-5. Reproduced under the Creative Commons license https://creativecommons.org/licenses/by/4.0/

    Kristensen JH et al. used ab102890 for the quantification of neutrophil elastase (NE)-degraded elastin (EL).

    EL-NE fragment levels in serum from patients with IPF (n = 10) compared with controls (n = 9).

    ** p < 0.01. (B): EL-NE fragment levels in serum from patients diagnosed with lung cancer (n = 40 in total), SCC (n = 16), adenocarcinoma (n = 16) and SCLC (n = 8) compared with controls (n = 12). **** p < 0.0001. Groups were compared by T-test with Welch correction. Data are shown as the geometric mean (95% CI).

    Abbreviations: IPF, idiopathic pulmonary fibrosis; SCC, squamous cell carcinoma; SCLC, small cell lung carcinoma.

  • HRP conjugation kit ab102890 was used to develop Frataxin ELISA kit ab176112
    HRP conjugation kit ab102890 was used to develop Frataxin ELISA kit ab176112

    HRP conjugation kit ab102890 is used by Abcam extensively in ELISA development. It is used for development / manufacturing of Abcam's SimpleStep ELISA® kits, including the Frataxin ELISA kit ab176112 used to produce the image above.

    Transformed B lymphocyte cells from Friedreich's Ataxia (FA) samples were compared to heterozygous carrier B lymphocyte cells (Carrier) and control B lymphocyte cells (Control). B lymphocyte cell extracts were analyzed across a 7-point titration (0.1-100 µg/mL) and frataxin levels were interpolated from the standard curve. Average interpolated values of Frataxin are plotted.

     

     

Protocols

  • Protocol Booklet - Chinese Version
  • Protocol Booklet

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (133)

Publishing research using ab102890? Please let us know so that we can cite the reference in this datasheet.

ab102890 has been referenced in 133 publications.

  • Miller CJ  et al. FN3-based monobodies selective for the receptor binding domain of the SARS-CoV-2 spike protein. N Biotechnol 62:79-85 (2021). PubMed: 33556628
  • Hallett ST  et al. Nse5/6 is a negative regulator of the ATPase activity of the Smc5/6 complex. Nucleic Acids Res 49:4534-4549 (2021). PubMed: 33849072
  • Zhu K  et al. Absence of microglia or presence of peripherally-derived macrophages does not affect tau pathology in young or old hTau mice. Glia 68:1466-1478 (2020). PubMed: 32039516
  • Tobajas AP  et al. Effect of high pressure and pulsed electric field on denaturation and allergenicity of Pru p 3 protein from peach. Food Chem 321:126745 (2020). PubMed: 32276148
  • Jonesco DS  et al. The CNS-specific proteoglycan, brevican, and its ADAMTS4-cleaved fragment show differential serological levels in Alzheimer's disease, other types of dementia and non-demented controls: A cross-sectional study. PLoS One 15:e0234632 (2020). PubMed: 32559242
View all Publications for this product

Customer reviews and Q&As

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1-5 of 5 Abreviews or Q&A

Good kit

Excellent Excellent 5/5 (Ease of Use)
Abreviews
Abreviews
I conducted an experiment using this kit.
Antibody and HRP were connected to see improved detection effect.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Oct 09 2019

signal slightly faint but viewable

Average Excellent 5/5 (Ease of Use)
Abreviews
Abreviews
abreview image
Tried this kit to conjugate the mouse antibody with HRP and used it for Western blotting with mouse tissue (5μg total protein), reacted in the dark for 3h in PBS. The signal appears to be slightly faint at the end.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Jan 21 2022

Question

Can you please tell me, if you have monoclonals antibodies against the proteins 3ABC of the Aftose virus, also conjugated antibodies with HRP and the substrate, please sent me the information of these products

Read More

Abcam community

Verified customer

Asked on May 21 2012

Answer

Thank you for contacting us.
As my colleague mentioned, we do not currently carry any antibodies to the Aftose virus. You may be able to find one by searching biocompare.com.
For the other products you mentioned, we do have a number of HRP conjugated secondary antibodies, as well as conjugation kits that can be used to label primary antibodies with HRP:
www.abcam.com/EasyLink
For an HRP substrate, we offer DAB detection kits for colorimetric studies (such as ab94665), and ECL substrates for western blotting (such as ab65623).
I hope this helps, please let me know if you need any additional information or assistance.

Read More

Abcam Scientific Support

Answered on May 21 2012

Question

Hi Tech, Can you recommend anything for my customer who intended to do Elisa. She also wanted to know the most sensitive way to detect her samples as she suspects the cell has little of the protein. Kindly reply the soonest. Thanks

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Abcam community

Verified customer

Asked on Dec 16 2011

Answer

Thank you for your inquiry. In case the customer would like to perform a sandwich ELISA (sELISA) for the EBV viral capsid antigen, I would suggest to test the antibody clones [2E08] and [8.F.90] (ab48414 and ab32803, see below). To our knowledge, this pair has not yet been tested in sELISA, and therefore, I can offer a discount off a future purchase if the customer buys them now, test them in sELISA and submit feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive the feedback. The discount would be to the value of 1 free primary antibody each. The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount. Please let me know if the customer is interested in this offer and I would be happy to help you further. If these antibodies are used in sELISA, one of them (the detection antibody) needs to be conjugated to an enzyme such as HRP and we would therefore recommend our EasyLink conjugation kits ab102889 or ab102890 (www.abcam.com/EasyLink or see below). If the customer is interested in one of these, I would be pleased to offer a discount. Unfortunately, we do not have any other antibodies available against EBV Ea-R p17 than ab64281 and against EBV Ribonucleotide Reductase than ab6504, respectively. Therefore, I cannot give a recommendation for a suitable paired antibody to use in sELISA. Having said this, I could recommend the HRP-conjugated secondary antibodies ab98693 (for the mouse-IgG1 antibody ab6504) and ab98698 (for the mouse-IgG2a antibody ab64281) in case the customer would like to set up a conventional indirect ELISA (see below). Using a secondary antibody amplifies the signal and this might be useful for proteins of low abundance. https://www.abcam.com/EBV-viral-capsid-antigen-antibody-M5042523-ab32803.html https://www.abcam.com/EBV-viral-capsid-antigen-antibody-M5042523-ab32803.html. https://www.abcam.com/EasyLink-HRP-Conjugation-Kit-1-x-1mg-HRP-ab102889.html https://www.abcam.com/EasyLink-HRP-Conjugation-Kit-1-x-1mg-HRP-ab102889.html. https://www.abcam.com/HRP-Conjugation-Kit-Lightning-Linkreg-ab102890.html https://www.abcam.com/HRP-Conjugation-Kit-Lightning-Linkreg-ab102890.html. https://www.abcam.com/Goat-Mouse-IgG1-HRP-preadsorbed-ab98693.html https://www.abcam.com/Goat-Mouse-IgG1-HRP-preadsorbed-ab98693.html. https://www.abcam.com/Goat-Mouse-IgG2a-heavy-chain-HRP-preadsorbed-ab98698.html https://www.abcam.com/Goat-Mouse-IgG2a-heavy-chain-HRP-preadsorbed-ab98698.html. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Abcam Scientific Support

Answered on Dec 16 2011

Question

LOT NUMBER GR29857 ORDER NUMBER 941272 DESCRIPTION OF THE PROBLEM No bands detectable in western blot (repeated twice), also after incubation with 2° antibody. After incubation with the first wash fraction from purification (instead of the HRP-conjugate) and the 2° antibody, there was a weak signal. SAMPLE Protein extract from rat intestinal mucosa (separate extracts for duodenum, jejunum, ileum, caecum, colon) PRIMARY ANTIBODY Anti-Vitamin D Receptor antibody [9A7gammaE10.4] (ab54387) I expected the 100 ug of antibody to be in the HRP-conjugate, which was 480 ul. According to this, I used a dilution of 1:100, incubation in 1% BSA overnight at 4°C, Three wash steps with PBST Loading control: mouse anti GAPDH (HRP), ab9482, Lot GR44318 POSITIVE AND NEGATIVE CONTROLS USED None ANTIBODY STORAGE CONDITIONS Storage at 4 °C on arrival, antibody purification (with the purification kit, ab102783, Lot GR36646) according to protocol (followed it exactly) one day after arrival and conjugation with HRP (EasyLink conjugation kit, ab102890, Lot GR51319) according to the protocol the following day, since then storage at 4°C. SAMPLE PREPARATION RIPA buffer with PMSF, leupeptin and aprotinin for extraction, incubation of samples with loading buffer for 5 minutes at 95°C before loading. AMOUNT OF PROTEIN LOADED 40ug/well ELECTROPHORESIS/GEL CONDITIONS Reducing 12% gel, 120 V TRANSFER AND BLOCKING CONDITIONS Transfer: wet, 135 mA, 90 minutes, Tris-glycine with 20% methanol, nitrocellulose membrane Ponceau S Red for protein detection (very nice signal) Blocking: 5% BSA in 0.1 % PBS Tween for 60 minutes at room temperature SECONDARY ANTIBODY GE healthcare amersham bisocience, goat anti-rat IgG, HRP-linked Dilution 1:5000 in 1% BSA, incubation for 90 minutes at room temperature HOW MANY TIMES HAVE YOU TRIED THE APPLICATION? 3 HAVE YOU RUN A "NO PRIMARY" CONTROL? No DO YOU OBTAIN THE SAME RESULTS EVERY TIME? No WHAT STEPS HAVE YOU ALTERED? the third time, I used the wash fraction from purification instead of the assumed HRP-antibody-conjugate, which gave me a weak signal. ADDITIONAL NOTES The loading control worked fine. i attached an image of the first attempt (loading control + membrane after incubation with HRP-antibody-conjugate) and of the third (membrane after incubation with wash fraction)

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Abcam community

Verified customer

Asked on Sep 21 2011

Answer

Thank you for contacting us. I am sorry to hear you are experiencing difficulties with some of our products. We take product complaints very seriously, and investigate every product that we feel may not be performing correctly. In this case the first thing I investigated was whether the purification kit is compatible with rat IgG, especially since a weak signal was observed with the wash fraction. In this case ab102783 contains protein A resin which shows no affinity for rat IgG therefore is not being effectively purified. This explains why there is a weak signal from the wash fraction as the antibody is being eluted and not captured effectively. Reviewing recent correspondence I can see that you had been in touch with us previously and directed to the purification and conjugation kits. I would like apologize that these products are not working for you and for any inconvenience that may have caused. I would be happy to reimburse you if we misled you in any way. Cross-reactivity with the rat samples was something you were concerned about as ab54387 is a rat antibody, however I do not think this would be a major problem. This may be true in IHC but in WB it is unlikely that endogenous Ig's will be present. Have you tried using ab54387 and a suitable secondary to check for cross-reactivity in the sample? If this is a problem there are some alternative primary antibodies I would like to recommend: ab3508 Anti-Vitamin D Receptor antibody https://www.abcam.com/Vitamin-D-Receptor-antibody-ab3508.html ab80666 Anti-Vitamin D Receptor antibody [9A7 gamma .E10.E4] - BSA and Azide free https://www.abcam.com/Vitamin-D-Receptor-antibody-9A7-gamma-E10E4-ab80666.html ab109234 Anti-Vitamin D Receptor antibody [EPR4552] https://www.abcam.com/Vitamin-D-Receptor-antibody-EPR4552-ChIP-Grade-ab109234.html ab80666 although again this is a rat monoclonal, it is BSA and Azide free therefore the purification kit would not be necessary and the antibody could be conjugated directly with the EasyLink HRP conjugation kit. This is something you may be interested in to remove the need for a compatible secondary antibody. Again I am sorry for any inconvenience and look forward to receiving your reply, so that we can resolve this matter as soon as possible.

Read More

Abcam Scientific Support

Answered on Sep 21 2011

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