Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- HRP Rabbit monoclonal [EPR9442(ABC)] to COX IV
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Rat, Human
- Conjugation: HRP
Product nameHRP Anti-COX IV antibody [EPR9442(ABC)]
See all COX IV primary antibodies
DescriptionHRP Rabbit monoclonal [EPR9442(ABC)] to COX IV
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human COX IV aa 1-100. The exact sequence is proprietary.
Database link: P13073
- WB: HepG2 whole cell and Mouse Heart tissue and Rat Heart tissue lysates IHC-P: normal human colon tissue sections
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark.
Storage bufferpH: 7.40
Preservative: 0.1% 10% Proclin 300 Solution
Constituents: 1% BSA, 30% Glycerol, PBS
Concentration information loading...
PurityProtein A purified
- Pathways and Processes
- Metabolic signaling pathways
- Energy transfer pathways
- Integration of energy
- Anti-COX IV antibody [EPR9442(ABC)] - Mitochondrial Loading Control (ab202554)
- Alexa Fluor® 405 Anti-COX IV antibody [EPR9442(ABC)] - Mitochondrial Loading Control (ab210180)
- Alexa Fluor® 594 Anti-COX IV antibody [EPR9442(ABC)] - Mitochondrial Loading Control (ab210674)
- Alexa Fluor® 555 Anti-COX IV antibody [EPR9442(ABC)] - Mitochondrial Loading Control (ab210675)
- Alexa Fluor® 568 Anti-COX IV antibody [EPR9442(ABC)] - Mitochondrial Loading Control (ab210848)
- Anti-COX IV antibody [EPR9442(ABC)] - BSA and Azide free (ab231168)
Our Abpromise guarantee covers the use of ab209958 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||1/5000. Detects a band of approximately 17 kDa (predicted molecular weight: 20 kDa).|
FunctionThis protein is one of the nuclear-coded polypeptide chains of cytochrome c oxidase, the terminal oxidase in mitochondrial electron transport.
Sequence similaritiesBelongs to the cytochrome c oxidase IV family.
Cellular localizationMitochondrion inner membrane.
- Information by UniProt
- AL024441 antibody
- COX 4 antibody
- COX IV 1 antibody
IHC image of COX IV staining in a section of formalin-fixed paraffin-embedded normal human colon*, performed on a Leica BOND™. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab209958, 1/100 dilution, for 15 mins at room temperature. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset negative control image is taken from an identical assay without primary antibody.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
All lanes : HRP Anti-COX IV antibody [EPR9442(ABC)] (ab209958) at 1/5000 dilution
Lane 1 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lane 2 : Heart (Mouse) Tissue Lysate
Lane 3 : Heart (Rat) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 20 kDa
Observed band size: 17 kDa why is the actual band size different from the predicted?
Exposure time: 5 seconds
This blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab209958 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.
ab209958 has not yet been referenced specifically in any publications.