HRP Anti-COX IV antibody [mAbcam33985] - Mitochondrial Marker (ab62164)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-COX IV antibody [mAbcam33985] - Mitochondrial Marker (ab62164)
  • Western blot - HRP Anti-COX IV antibody [mAbcam33985] - Mitochondrial Marker (ab62164)
  • Western blot - HRP Anti-COX IV antibody [mAbcam33985] - Mitochondrial Marker (ab62164)

Key features and details

  • HRP Mouse monoclonal [mAbcam33985] to COX IV - Mitochondrial Marker
  • Suitable for: WB, IHC-P
  • Reacts with: Mouse, Human
  • Conjugation: HRP
  • Isotype: IgG

Overview

  • Product name

    HRP Anti-COX IV antibody [mAbcam33985] - Mitochondrial Marker
    See all COX IV primary antibodies

  • Description

    HRP Mouse monoclonal [mAbcam33985] to COX IV - Mitochondrial Marker

  • Host species

    Mouse

  • Conjugation

    HRP

  • Tested applications

    Suitable for: WB, IHC-Pmore details

  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat, Cow, Xenopus laevis, Chimpanzee, Zebrafish

  • Immunogen

    Synthetic peptide corresponding to Human COX IV aa 150 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab16381)

  • Positive control

    • WB: HeLa, HEK293, A431, Jurkat and HepG2 whole cell lysates and mouse skeletal muscle tissue lysate. IHC-P: Normal human colon tissue.

  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Applications

Our Abpromise guarantee covers the use of ab62164 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/5000. Predicted molecular weight: 15 kDa.
IHC-P 1/100.

Target

  • Function

    This protein is one of the nuclear-coded polypeptide chains of cytochrome c oxidase, the terminal oxidase in mitochondrial electron transport.

  • Tissue specificity

    Ubiquitous.

  • Sequence similarities

    Belongs to the cytochrome c oxidase IV family.

  • Cellular localization

    Mitochondrion inner membrane.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • AL024441 antibody
    • COX 4 antibody
    • COX IV 1 antibody
    • COX IV antibody
    • COX IV-1 antibody
    • Cox4 antibody
    • COX41_HUMAN antibody
    • Cox4a antibody
    • COX4B antibody
    • COX4I1 antibody
    • COX4I2 antibody
    • COX4L2 antibody
    • COXIV antibody
    • Cytochrome c oxidase polypeptide IV antibody
    • Cytochrome c oxidase subunit 4 isoform 1 mitochondrial antibody
    • Cytochrome c oxidase subunit 4 isoform 1, mitochondrial antibody
    • Cytochrome C Oxidase subunit IV antibody
    • Cytochrome c oxidase subunit IV isoform 1 antibody
    • Cytochrome c oxidase subunit IV isoform 2 (lung) antibody
    • Cytochrome c oxydase subunit 4 antibody
    • dJ857M17.2 antibody
    • MGC105470 antibody
    • MGC72016 antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-COX IV antibody [mAbcam33985] - Mitochondrial Marker (ab62164)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-COX IV antibody [mAbcam33985] - Mitochondrial Marker (ab62164)

    IHC image of COX IV staining in a section of formalin-fixed paraffin-embedded normal human colon*, performed on a Leica BONDTM. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab62164 at 1/100 dilution, for 15 mins at room temperature. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    The inset negative control image is taken from an identical assay without primary antibody.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre.

  • Western blot - HRP Anti-COX IV antibody [mAbcam33985] - Mitochondrial Marker (ab62164)
    Western blot - HRP Anti-COX IV antibody [mAbcam33985] - Mitochondrial Marker (ab62164)
    All lanes : HRP Anti-COX IV antibody [mAbcam33985] - Mitochondrial Marker (ab62164) at 1/5000 dilution

    Lane 1 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
    Lane 2 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
    Lane 3 : Skeletal Muscle (Mouse) Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 15 kDa
    Observed band size: 15 kDa


    Exposure time: 2 minutes


    This blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab62164 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.

  • Western blot - HRP Anti-COX IV antibody [mAbcam33985] - Mitochondrial Marker (ab62164)
    Western blot - HRP Anti-COX IV antibody [mAbcam33985] - Mitochondrial Marker (ab62164)
    All lanes : HRP Anti-COX IV antibody [mAbcam33985] - Mitochondrial Marker (ab62164) at 1/4000 dilution

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
    Lane 3 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 4 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Performed under reducing conditions.

    Predicted band size: 15 kDa
    Observed band size: 17 kDa
    why is the actual band size different from the predicted?

References (4)

Publishing research using ab62164? Please let us know so that we can cite the reference in this datasheet.

ab62164 has been referenced in 4 publications.

  • Lobo-Jarne T  et al. Multiple pathways coordinate assembly of human mitochondrial complex IV and stabilization of respiratory supercomplexes. EMBO J N/A:e103912 (2020). PubMed: 32511785
  • Serricchio M  et al. Cardiolipin synthesizing enzymes form a complex that interacts with cardiolipin-dependent membrane organizing proteins. Biochim Biophys Acta 1863:447-457 (2018). PubMed: 29343430
  • Di K  et al. Mitochondrial Lon is over-expressed in high-grade gliomas, and mediates hypoxic adaptation: potential role of Lon as a therapeutic target in glioma. Oncotarget 7:77457-77467 (2016). WB ; Human . PubMed: 27764809
  • Powell KA  et al. Mitochondrial localization and ocular expression of mutant Opa3 in a mouse model of 3-methylglutaconicaciduria type III. Invest Ophthalmol Vis Sci 52:4369-80 (2011). PubMed: 21613372

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