Recombinant HRP Anti-CPS1 antibody [EPR7493-3] (ab198969)
![Western blot - HRP Anti-CPS1 antibody [EPR7493-3] (ab198969)](https://www.abcam.com/ps/products/198/ab198969/Images/ab198969-251062-anti-cps1-antibody-epr7493-3-hrp-western-blot.jpg "Western blot - HRP Anti-CPS1 antibody [EPR7493-3] (ab198969)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- HRP Rabbit monoclonal [EPR7493-3] to CPS1
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human
- Conjugation: HRP
Overview
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Product name
HRP Anti-CPS1 antibody [EPR7493-3]
See all CPS1 primary antibodies -
Description
HRP Rabbit monoclonal [EPR7493-3] to CPS1 -
Host species
Rabbit -
Conjugation
HRP -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide within Human CPS1 aa 100-200. The exact sequence is proprietary.
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Positive control
- WB: Human fetal liver, mouse liver and rat liver tissue lysates. IHC-P: normal human liver tissue sections.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.1% 10% Proclin 300 Solution
Constituents: PBS, 1% BSA, 30% Glycerol -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR7493-3 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
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Related Products
Applications
Our Abpromise guarantee covers the use of ab198969 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/5000. Detects a band of approximately 165 kDa (predicted molecular weight: 165 kDa). | |
| IHC-P | 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
Involved in the urea cycle of ureotelic animals where the enzyme plays an important role in removing excess ammonia from the cell. -
Tissue specificity
Primarily in the liver and small intestine. -
Involvement in disease
Defects in CPS1 are the cause of carbamoyl phosphate synthetase 1 deficiency (CPS1D) [MIM:237300]. CPS1D is an autosomal recessive disorder of the urea cycle causing hyperammonemia. Clinical features include protein intolerance, intermittent ataxia, seizures, lethargy, developmental delay and mental retardation.
Note=Genetic variations in CPS1 influence the availability of precursors for nitric oxide (NO) synthesis and play a role in clinical situations where endogenous NO production is critically important, such as neonatal pulmonary hypertension, increased pulmonary artery pressure following surgical repair of congenital heart defects or hepatovenocclusive disease following bone marrow transplantation. Infants with neonatal pulmonary hypertension homozygous for Thr-1406 have lower L-arginine concentrations than neonates homozygous for Asn-1406. -
Sequence similarities
Contains 2 ATP-grasp domains.
Contains 1 glutamine amidotransferase type-1 domain. -
Domain
The type-1 glutamine amidotransferase domain is defective. -
Cellular localization
Mitochondrion. - Information by UniProt
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Database links
- Entrez Gene: 1373 Human
- Entrez Gene: 227231 Mouse
- Entrez Gene: 497840 Rat
- Omim: 608307 Human
- SwissProt: P31327 Human
- SwissProt: Q8C196 Mouse
- SwissProt: P07756 Rat
- Unigene: 149252 Human
see all -
Alternative names
- Carbamoyl phosphate synthase [ammonia] antibody
- Carbamoyl phosphate synthase [ammonia] mitochondrial antibody
- Carbamoyl phosphate synthase antibody
see all
Images
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Western blot - HRP Anti-CPS1 antibody [EPR7493-3] (ab198969)All lanes : HRP Anti-CPS1 antibody [EPR7493-3] (ab198969) at 1/5000 dilution
Lane 1 : Fetal Liver (Human) Normal Tissue Lysate
Lane 2 : Liver (Mouse) Tissue Lysate
Lane 3 : Liver (Rat) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 165 kDa
Observed band size: 165 kDa
Exposure time: 30 secondsThis blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab198969 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-CPS1 antibody [EPR7493-3] (ab198969)](https://www.abcam.com/ps/products/198/ab198969/Images/ab198969-242225-anti-cps1-antibody-epr7493-3-hrp-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-CPS1 antibody [EPR7493-3] (ab198969)IHC image of CPS1 staining in a section of formalin-fixed paraffin-embedded normal human liver tissue*, performed on a Leica BOND. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab198969 at 1/100 dilution, for 15 mins at room temperature. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset negative control image is taken from an identical assay without primary antibody.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre.
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![HRP Anti-CPS1 antibody [EPR7493-3] (ab198969)](https://www.abcam.com/ps/products/198/ab198969/Images/ab198969-3-benefits-of-recombinant-antibodies.png)
HRP Anti-CPS1 antibody [EPR7493-3] (ab198969)
Protocols
References (0)
ab198969 has not yet been referenced specifically in any publications.
