Recombinant HRP Anti-CSN8 antibody [EPR5139] (ab208842)
![Western blot - HRP Anti-CSN8 antibody [EPR5139] (ab208842)](https://www.abcam.com/ps/products/208/ab208842/Images/ab208842-270861-anti-cops8-antibody-epr5139-hrp-western-blot.jpg "Western blot - HRP Anti-CSN8 antibody [EPR5139] (ab208842)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- HRP Rabbit monoclonal [EPR5139] to CSN8
- Suitable for: WB
- Reacts with: Human
- Conjugation: HRP
Overview
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Product name
HRP Anti-CSN8 antibody [EPR5139]
See all CSN8 primary antibodies -
Description
HRP Rabbit monoclonal [EPR5139] to CSN8 -
Host species
Rabbit -
Conjugation
HRP -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat
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Immunogen
within Human CSN8 aa 150 to the C-terminus. The exact sequence is proprietary.
Database link: Q99627 -
Positive control
- WB: HeLa and SH-SY5Y whole cell and Human Fetal Liver tissue lysates.
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General notes
This product was previously labelled as COPS8
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.1% 10% Proclin 300 Solution
Constituents: 30% Glycerol, 1% BSA, PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR5139 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab208842 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/5000. Detects a band of approximately 21 kDa (predicted molecular weight: 23 kDa). |
Target
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Function
Component of the COP9 signalosome complex (CSN), a complex involved in various cellular and developmental processes. The CSN complex is an essential regulator of the ubiquitin (Ubl) conjugation pathway by mediating the deneddylation of the cullin subunits of SCF-type E3 ligase complexes, leading to decrease the Ubl ligase activity of SCF-type complexes such as SCF, CSA or DDB2. The complex is also involved in phosphorylation of p53/TP53, c-jun/JUN, IkappaBalpha/NFKBIA, ITPK1 and IRF8/ICSBP, possibly via its association with CK2 and PKD kinases. CSN-dependent phosphorylation of TP53 and JUN promotes and protects degradation by the Ubl system, respectively. -
Sequence similarities
Belongs to the CSN8 family.
Contains 1 PCI domain. -
Cellular localization
Cytoplasm. Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 10920 Human
- Entrez Gene: 108679 Mouse
- Entrez Gene: 363283 Rat
- Omim: 616011 Human
- SwissProt: Q99627 Human
- SwissProt: Q8VBV7 Mouse
- SwissProt: Q6P4Z9 Rat
- Unigene: 531713 Human
see all -
Alternative names
- COP9 homolog antibody
- COP9 signalosome complex subunit 8 antibody
- COP9 signalosome subunit 8 antibody
see all
Images
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Western blot - HRP Anti-CSN8 antibody [EPR5139] (ab208842)All lanes : HRP Anti-CSN8 antibody [EPR5139] (ab208842) at 1/5000 dilution
Lane 1 :HeLa whole cell lysate (ab150035)
Lane 2 : Liver (Human) Tissue Lysate - fetal normal tissue
Lane 3 : SH-SY5Y (Human neuroblastoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 23 kDa
Observed band size: 21 kDa why is the actual band size different from the predicted?
Exposure time: 12 minutesThis blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab208842 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.
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![HRP Anti-CSN8 antibody [EPR5139] (ab208842)](https://www.abcam.com/ps/products/208/ab208842/Images/ab208842-2-benefits-of-recombinant-antibodies.png)
HRP Anti-CSN8 antibody [EPR5139] (ab208842)
References (0)
ab208842 has not yet been referenced specifically in any publications.
