Key features and details
- HRP Rabbit monoclonal [EPR8837] to CXCR5
- Suitable for: WB
- Reacts with: Human
- Conjugation: HRP
Product nameHRP Anti-CXCR5 antibody [EPR8837]
See all CXCR5 primary antibodies
DescriptionHRP Rabbit monoclonal [EPR8837] to CXCR5
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
Synthetic peptide within Human CXCR5 aa 350 to the C-terminus (C terminal). The exact sequence is proprietary.
- WB: Raji, MOLT4 and HeLa whole cell lysates.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark.
Dissociation constant (KD)KD = 2.15 x 10 -11 M Learn more about KD
Storage bufferpH: 7.40
Preservative: 0.1% 10% Proclin 300 Solution
Constituents: PBS, 1% BSA, 30% Glycerol
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab198964 in the following tested applications.
|WB||1/5000. Detects a band of approximately 42 kDa (predicted molecular weight: 42 kDa).|
FunctionCytokine receptor that binds to B-lymphocyte chemoattractant (BLC). Involved in B-cell migration into B-cell follicles of spleen and Peyer patches but not into those of mesenteric or peripheral lymph nodes. May have a regulatory function in Burkitt lymphoma (BL) lymphomagenesis and/or B-cell differentiation.
Tissue specificityExpression in mature B-cells and Burkitt lymphoma cells.
Sequence similaritiesBelongs to the G-protein coupled receptor 1 family.
Cellular localizationCell membrane.
- Information by UniProt
- BLR 1 antibody
- BLR1 antibody
- Burkitt lymphoma receptor 1 antibody
All lanes : HRP Anti-CXCR5 antibody [EPR8837] (ab198964) at 1/5000 dilution
Lane 1 : Raji (Human Burkitt's lymphoma cell line) Whole Cell Lysate
Lane 2 : MOLT4 (Human acute lymphoblastic leukemia cell line) Whole Cell Lysate
Lane 3 :
HeLa whole cell lysate (ab150035)
Lysates/proteins at 10 µg per lane.
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 42 kDa
Observed band size: 42 kDa
Exposure time: 30 seconds
This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab198964 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.
ab198964 has not yet been referenced specifically in any publications.