Recombinant HRP Anti-Cyclin A2 antibody [E399] (ab193598)
![Western blot - HRP Anti-Cyclin A2 antibody [E399] (ab193598)](https://www.abcam.com/ps/products/193/ab193598/Images/ab193598-238528-anti-cyclin-a2-antibody-e399-hrp-western-blot.jpg "Western blot - HRP Anti-Cyclin A2 antibody [E399] (ab193598)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- HRP Rabbit monoclonal [E399] to Cyclin A2
- Suitable for: WB
- Reacts with: Human
- Conjugation: HRP
Related conjugates and formulations
Overview
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Product name
HRP Anti-Cyclin A2 antibody [E399]
See all Cyclin A2 primary antibodies -
Description
HRP Rabbit monoclonal [E399] to Cyclin A2 -
Host species
Rabbit -
Conjugation
HRP -
Tested applications
Suitable for: WBmore details
Unsuitable for: IHC-P -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Epitope
ab32498 reacts with an epitope located in the N terminal region of Cyclin A2 -
Positive control
- WB: HeLa whole cell lysate.
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.1% Proclin 300 Solution
Constituents: 30% Glycerol (glycerin, glycerine), 1% BSA, PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
E399 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab193598 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000. Detects a band of approximately 52 kDa (predicted molecular weight: 48 kDa).
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| Notes |
|---|
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WB
1/1000. Detects a band of approximately 52 kDa (predicted molecular weight: 48 kDa). |
Target
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Function
Essential for the control of the cell cycle at the G1/S (start) and the G2/M (mitosis) transitions. -
Sequence similarities
Belongs to the cyclin family. Cyclin AB subfamily. -
Developmental stage
Accumulates steadily during G2 and is abruptly destroyed at mitosis. -
Cellular localization
Nucleus. Cytoplasm. Cytoplasmic when associated with SCAPER. - Information by UniProt
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Database links
- Entrez Gene: 890 Human
- Omim: 123835 Human
- SwissProt: P20248 Human
- Unigene: 58974 Human
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Alternative names
- CCN1 antibody
- CCNA antibody
- Ccna2 antibody
see all
Images
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Western blot - HRP Anti-Cyclin A2 antibody [E399] (ab193598)HRP Anti-Cyclin A2 antibody [E399] (ab193598) at 1/1000 dilution +
HeLa whole cell lysate (ab150035) at 10 µg
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 48 kDa
Observed band size: 52 kDa why is the actual band size different from the predicted?
Exposure time: 20 minutesThis blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab193598 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.
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![HRP Anti-Cyclin A2 antibody [E399] (ab193598)](https://www.abcam.com/ps/products/193/ab193598/Images/ab193598-3-benefits-of-recombinant-antibodies.png)
HRP Anti-Cyclin A2 antibody [E399] (ab193598)
Protocols
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (0)
ab193598 has not yet been referenced specifically in any publications.