Recombinant HRP Anti-Cyclin B1 antibody [Y106] (ab193977)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- HRP Rabbit monoclonal [Y106] to Cyclin B1
- Suitable for: WB
- Knockout validated
- Reacts with: Human
- Conjugation: HRP
Related conjugates and formulations
Overview
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Product name
HRP Anti-Cyclin B1 antibody [Y106]
See all Cyclin B1 primary antibodies -
Description
HRP Rabbit monoclonal [Y106] to Cyclin B1 -
Host species
Rabbit -
Conjugation
HRP -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human
Does not react with: Mouse, Rat -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Epitope
ab32053 reacts with an epitope located in teh C terminal region of Cyclin B1. -
Positive control
- Jurkat and Hela cell lysate.
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.1% Proclin 300 Solution
Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
Y106 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab193977 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/5000. Predicted molecular weight: 58 kDa.
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Notes |
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WB
1/5000. Predicted molecular weight: 58 kDa. |
Target
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Function
Essential for the control of the cell cycle at the G2/M (mitosis) transition. -
Sequence similarities
Belongs to the cyclin family. Cyclin AB subfamily. -
Developmental stage
Accumulates steadily during G2 and is abruptly destroyed at mitosis. -
Post-translational
modificationsUbiquitinated by the SCF(NIPA) complex during interphase, leading to its destruction. Not ubiquitinated during G2/M phases. -
Cellular localization
Cytoplasm. Nucleus. Cytoplasm > cytoskeleton > centrosome. - Information by UniProt
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Database links
- Entrez Gene: 891 Human
- Omim: 123836 Human
- SwissProt: P14635 Human
- Unigene: 23960 Human
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Alternative names
- CCNB 1 antibody
- CCNB antibody
- ccnb1 antibody
see all
Images
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All lanes : HRP Anti-Cyclin B1 antibody [Y106] (ab193977) at 1/5000 dilution
Lane 1 : Wild-type HAP1 whole cell lysate
Lane 2 : CCNB1 (Cyclin B1) knockout HAP1 whole cell lysate
Lysates/proteins at 20 µg per lane.
Predicted band size: 58 kDa
Observed band size: 51 kDa why is the actual band size different from the predicted?
Exposure time: 1 minuteab193977 was shown to specifically react with Cyclin B1 in wild-type HAP1 cells as signal was lost in CCNB1 (Cyclin B1) knockout cells. Wild-type and CCNB1 (Cyclin B1) knockout samples were subjected to SDS-PAGE. Ab193977 and ab184095 (Mouse monoclonal [mAbcam 9484] to GAPDH - Loading Control (Alexa Fluor® 680) loading control) were incubated overnight at 4°C at 1/5000 dilution and 1/1000 dilution respectively. The loading control was imaged using the Licor Odyssey CLx prior to blots being developed with ECL technique.
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All lanes : HRP Anti-Cyclin B1 antibody [Y106] (ab193977) at 1/5000 dilution
Lane 1 :HeLa whole cell lysate (ab150035)
Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 58 kDa
Observed band size: 58 kDa
Exposure time: 1 minuteThis blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab193977 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (0)
ab193977 has not yet been referenced specifically in any publications.