Recombinant HRP Anti-Cyclin B1 antibody [Y106] (ab193977)
![Western blot - HRP Anti-Cyclin B1 antibody [Y106] (ab193977)](https://www.abcam.com/ps/products/193/ab193977/Images/ab193977-312023-anti-cyclin-b1-antibody-y106-hrp-western-blot.jpg "Western blot - HRP Anti-Cyclin B1 antibody [Y106] (ab193977)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- HRP Rabbit monoclonal [Y106] to Cyclin B1
- Suitable for: IHC-P, WB
- Knockout validated
- Reacts with: Human
- Conjugation: HRP
Overview
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Product name
HRP Anti-Cyclin B1 antibody [Y106]
See all Cyclin B1 primary antibodies -
Description
HRP Rabbit monoclonal [Y106] to Cyclin B1 -
Host species
Rabbit -
Conjugation
HRP -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Human
Does not react with: Mouse, Rat -
Immunogen
Synthetic peptide within Human Cyclin B1 aa 400 to the C-terminus (C terminal). The exact sequence is proprietary.
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Epitope
ab32053 reacts with an epitope located in teh C terminal region of Cyclin B1. -
Positive control
- Jurkat and Hela cell lysate. IHC-P: Human FFPE colon adenocarcinoma tissue sections.
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.1% 10% Proclin 300 Solution
Constituents: PBS, 30% Glycerol, 1% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
Y106 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab193977 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | 1/100. | |
| WB | 1/5000. Predicted molecular weight: 58 kDa. |
Target
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Function
Essential for the control of the cell cycle at the G2/M (mitosis) transition. -
Sequence similarities
Belongs to the cyclin family. Cyclin AB subfamily. -
Developmental stage
Accumulates steadily during G2 and is abruptly destroyed at mitosis. -
Post-translational
modificationsUbiquitinated by the SCF(NIPA) complex during interphase, leading to its destruction. Not ubiquitinated during G2/M phases. -
Cellular localization
Cytoplasm. Nucleus. Cytoplasm > cytoskeleton > centrosome. - Information by UniProt
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Database links
- Entrez Gene: 891 Human
- Omim: 123836 Human
- SwissProt: P14635 Human
- Unigene: 23960 Human
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Alternative names
- CCNB 1 antibody
- CCNB antibody
- ccnb1 antibody
see all
Images
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Western blot - HRP Anti-Cyclin B1 antibody [Y106] (ab193977)All lanes : HRP Anti-Cyclin B1 antibody [Y106] (ab193977) at 1/5000 dilution
Lane 1 : Wild-type HAP1 whole cell lysate
Lane 2 : CCNB1 (Cyclin B1) knockout HAP1 whole cell lysate
Lysates/proteins at 20 µg per lane.
Predicted band size: 58 kDa
Observed band size: 51 kDa why is the actual band size different from the predicted?
Exposure time: 1 minuteab193977 was shown to specifically react with Cyclin B1 in wild-type HAP1 cells as signal was lost in CCNB1 (Cyclin B1) knockout cells. Wild-type and CCNB1 (Cyclin B1) knockout samples were subjected to SDS-PAGE. Ab193977 and ab184095 (Mouse monoclonal [mAbcam 9484] to GAPDH - Loading Control (Alexa Fluor® 680) loading control) were incubated overnight at 4°C at 1/5000 dilution and 1/1000 dilution respectively. The loading control was imaged using the Licor Odyssey CLx prior to blots being developed with ECL technique.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-Cyclin B1 antibody [Y106] (ab193977)](https://www.abcam.com/ps/products/193/ab193977/Images/ab193977-232140-anti-cyclin-b1-antibody-y106-hrp-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-Cyclin B1 antibody [Y106] (ab193977)IHC image of Cyclin B1 staining in a section of formalin-fixed paraffin-embedded human colon adenocarcinoma*. The section was pre-treated using pressure cooker heat mediated antigen retrieval with sodium citrate buffer (pH6) for 30mins. The section was incubated with ab193977, at a working dilution of 1/100 overnight at +4°C. The section was counterstained with haematoxylin and mounted with DPX. The inset negative control image is taken from an identical assay without primary antibody.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
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![Western blot - HRP Anti-Cyclin B1 antibody [Y106] (ab193977)](https://www.abcam.com/ps/products/193/ab193977/Images/ab193977-232193-anti-cyclin-b1-antibody-y106-hrp-western-blot.jpg)
Western blot - HRP Anti-Cyclin B1 antibody [Y106] (ab193977)All lanes : HRP Anti-Cyclin B1 antibody [Y106] (ab193977) at 1/5000 dilution
Lane 1 :HeLa whole cell lysate (ab150035)
Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 58 kDa
Observed band size: 58 kDa
Exposure time: 1 minuteThis blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab193977 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.
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![HRP Anti-Cyclin B1 antibody [Y106] (ab193977)](https://www.abcam.com/ps/products/193/ab193977/Images/ab193977-4-benefits-of-recombinant-antibodies.png)
HRP Anti-Cyclin B1 antibody [Y106] (ab193977)
Protocols
References (0)
ab193977 has not yet been referenced specifically in any publications.
