Recombinant HRP Anti-Cytochrome P450 17A1/CYP17A1 antibody [EPR6293] (ab204022)
![Western blot - HRP Anti-Cytochrome P450 17A1/CYP17A1 antibody [EPR6293] (ab204022)](https://www.abcam.com/ps/products/204/ab204022/Images/ab204022-254136-WBab2040221.jpg "Western blot - HRP Anti-Cytochrome P450 17A1/CYP17A1 antibody [EPR6293] (ab204022)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- HRP Rabbit monoclonal [EPR6293] to Cytochrome P450 17A1/CYP17A1
- Suitable for: WB
- Reacts with: Human
- Conjugation: HRP
Overview
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Product name
HRP Anti-Cytochrome P450 17A1/CYP17A1 antibody [EPR6293]
See all Cytochrome P450 17A1/CYP17A1 primary antibodies -
Description
HRP Rabbit monoclonal [EPR6293] to Cytochrome P450 17A1/CYP17A1 -
Host species
Rabbit -
Conjugation
HRP -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Cytochrome P450 17A1/CYP17A1. The exact sequence is proprietary.
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Positive control
- WB: HeLa and Jurkat whole cell lysates.
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General notes
This product was previously labelled as Cytochrome P450 17A1
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.1% 10% Proclin 300 Solution
Constituents: PBS, 30% Glycerol, 1% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR6293 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
- Anti-Cytochrome P450 17A1/CYP17A1 antibody [EPR6293] (ab125022)
- Alexa Fluor® 647 Anti-Cytochrome P450 17A1/CYP17A1 antibody [EPR6293] (ab203743)
- Alexa Fluor® 488 Anti-Cytochrome P450 17A1/CYP17A1 antibody [EPR6293] (ab203995)
- PE Anti-Cytochrome P450 17A1/CYP17A1 antibody [EPR6293] (ab223958)
- Anti-Cytochrome P450 17A1/CYP17A1 antibody [EPR6293] - Low endotoxin, Azide free (ab226009)
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
Our Abpromise guarantee covers the use of ab204022 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/5000. Detects a band of approximately 57 kDa (predicted molecular weight: 57 kDa). |
Target
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Function
Conversion of pregnenolone and progesterone to their 17-alpha-hydroxylated products and subsequently to dehydroepiandrosterone (DHEA) and androstenedione. Catalyzes both the 17-alpha-hydroxylation and the 17,20-lyase reaction. Involved in sexual development during fetal life and at puberty. -
Pathway
Lipid metabolism; steroid biosynthesis. -
Involvement in disease
Defects in CYP17A1 are the cause of adrenal hyperplasia type 5 (AH5) [MIM:202110]. AH5 is a form of congenital adrenal hyperplasia, a common recessive disease due to defective synthesis of cortisol. Congenital adrenal hyperplasia is characterized by androgen excess leading to ambiguous genitalia in affected females, rapid somatic growth during childhood in both sexes with premature closure of the epiphyses and short adult stature. Four clinical types: "salt wasting" (SW, the most severe type), "simple virilizing" (SV, less severely affected patients), with normal aldosterone biosynthesis, "non-classic form" or late onset (NC or LOAH), and "cryptic" (asymptomatic). -
Sequence similarities
Belongs to the cytochrome P450 family. -
Post-translational
modificationsPhosphorylation is necessary for 17,20-lyase, but not for 17-alpha-hydroxylase activity. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 1586 Human
- Omim: 609300 Human
- SwissProt: P05093 Human
- Unigene: 438016 Human
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Alternative names
- 20 lyase antibody
- CP17A_HUMAN antibody
- CPT7 antibody
see all
Images
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Western blot - HRP Anti-Cytochrome P450 17A1/CYP17A1 antibody [EPR6293] (ab204022)All lanes : HRP Anti-Cytochrome P450 17A1/CYP17A1 antibody [EPR6293] (ab204022) at 1/5000 dilution
Lane 1 :HeLa whole cell lysate (ab150035)
Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 57 kDa
Observed band size: 57 kDa
Exposure time: 3 minutesThis blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab204022 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.
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![HRP Anti-Cytochrome P450 17A1/CYP17A1 antibody [EPR6293] (ab204022)](https://www.abcam.com/ps/products/204/ab204022/Images/ab204022-3-benefits-of-recombinant-antibodies.png)
HRP Anti-Cytochrome P450 17A1/CYP17A1 antibody [EPR6293] (ab204022)
References (0)
ab204022 has not yet been referenced specifically in any publications.
