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Signal Transduction Cytoskeleton / ECM Cytoskeleton Intermediate Filaments Class I Cytokeratins
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RecombinantRabMAb

Recombinant HRP Anti-Cytokeratin 16/K16 antibody [EP1615Y] (ab206064)

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  • SDS
  • Certificate of Compliance
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Western blot - HRP Anti-Cytokeratin 16/K16 antibody [EP1615Y] (ab206064)
  • HRP Anti-Cytokeratin 16/K16 antibody [EP1615Y] (ab206064)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • HRP Rabbit monoclonal [EP1615Y] to Cytokeratin 16/K16
  • Suitable for: WB
  • Reacts with: Human
  • Conjugation: HRP

You may also be interested in

Protein
Product image
Recombinant Human Cytokeratin 16/K16 protein (ab114405)
Primary
Product image
Anti-Cytokeratin 16/K16 antibody [EP1615Y] - BSA and Azide free (ab232522)

View more associated products

Overview

  • Product name

    HRP Anti-Cytokeratin 16/K16 antibody [EP1615Y]
    See all Cytokeratin 16/K16 primary antibodies
  • Description

    HRP Rabbit monoclonal [EP1615Y] to Cytokeratin 16/K16
  • Host species

    Rabbit
  • Conjugation

    HRP
  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HaCaT whole cell lysate.
  • General notes

    Previously labelled as Cytokeratin 16. 

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark.
  • Storage buffer

    pH: 7.40
    Preservative: 0.1% 10% Proclin 300 Solution
    Constituents: 30% Glycerol (glycerin, glycerine), 1% BSA, PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EP1615Y
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Cytoskeleton / ECM
    • Cytoskeleton
    • Intermediate Filaments
    • Class I
    • Cytokeratins

Associated products

  • Alternative Versions

    • Alexa Fluor® 488 Anti-Cytokeratin 16/K16 antibody [EP1615Y] (ab205744)
    • Alexa Fluor® 647 Anti-Cytokeratin 16/K16 antibody [EP1615Y] (ab205745)
    • Alexa Fluor® 555 Anti-Cytokeratin 16/K16 antibody [EP1615Y] (ab215292)
    • Anti-Cytokeratin 16/K16 antibody [EP1615Y] - BSA and Azide free (ab232522)
    • Anti-Cytokeratin 16/K16 antibody [EP1615Y] (ab76416)
  • Isotype control

    • HRP Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab199507)
  • Recombinant Protein

    • Recombinant Human Cytokeratin 16/K16 protein (ab114405)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab206064 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
WB
Human
Application Abreviews Notes
WB
1/5000. Detects a band of approximately 51 kDa (predicted molecular weight: 51 kDa).
Notes
WB
1/5000. Detects a band of approximately 51 kDa (predicted molecular weight: 51 kDa).

Target

  • Tissue specificity

    Expressed in the hair follicle, nail bed and in mucosal stratified squamous epithelia and, suprabasally, in oral epithelium and palmoplantar epidermis. Also found in luminal cells of sweat and mammary gland ducts.
  • Involvement in disease

    Defects in KRT16 are a cause of pachyonychia congenita type 1 (PC1) [MIM:167200]; also known as Jadassohn-Lewandowsky syndrome. PC1 is an autosomal dominant ectodermal dysplasia characterized by hypertrophic nail dystrophy resulting in onchyogryposis (thickening and increase in curvature of the nail), palmoplantar keratoderma, follicular hyperkeratosis, and oral leukokeratosis. Hyperhidrosis of the hands and feet is usually present.
    Defects in KRT16 are the cause of palmoplantar keratoderma non-epidermolytic focal (FNEPPK) [MIM:613000]. A dermatological disorder characterized by non-epidermolytic palmoplantar keratoderma limited to the pressure points on the balls of the feet, with later mild involvement on the palms. Oral, genital and follicular keratotic lesions are often present.
    Defects in KRT16 are a cause of unilateral palmoplantar verrucous nevus (UPVN) [MIM:144200]. UPVN is characterized by a localized thickening of the skin in parts of the right palm and the right sole.
    Note=KRT16 and KRT17 are coexpressed only in pathological situations such as metaplasias and carcinomas of the uterine cervix and in psoriasis vulgaris.
  • Sequence similarities

    Belongs to the intermediate filament family.
  • Target information above from: UniProt accession P08779 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 3868 Human
    • Omim: 148067 Human
    • SwissProt: P08779 Human
    • Unigene: 655160 Human
    • Alternative names

      • CK 16 antibody
      • CK-16 antibody
      • CK16 antibody
      • Cytokeratin 16 antibody
      • Cytokeratin-16 antibody
      • Cytokeratin16 antibody
      • FNEPPK antibody
      • Focal non epidermolytic palmoplantar keratoderma antibody
      • K 16 antibody
      • K16 antibody
      • K1C16_HUMAN antibody
      • K1CP antibody
      • Keratin 1 type I antibody
      • Keratin 16 antibody
      • Keratin antibody
      • Keratin type I cytoskeletal 16 antibody
      • Keratin-16 antibody
      • Keratin16 antibody
      • KRT 16 antibody
      • Krt16 antibody
      • KRT16A antibody
      • NEPPK antibody
      • PC1 antibody
      • type I cytoskeletal 16 antibody
      see all

    Images

    • Western blot - HRP Anti-Cytokeratin 16/K16 antibody [EP1615Y] (ab206064)
      Western blot - HRP Anti-Cytokeratin 16/K16 antibody [EP1615Y] (ab206064)
      HRP Anti-Cytokeratin 16/K16 antibody [EP1615Y] (ab206064) at 1/5000 dilution + HaCaT (Human keratinocyte cell line) Whole Cell Lysate at 10 µg

      Developed using the ECL technique.

      Performed under reducing conditions.

      Predicted band size: 51 kDa
      Observed band size: 51 kDa


      Exposure time: 16 minutes


      This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab206064 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.

    • HRP Anti-Cytokeratin 16/K16 antibody [EP1615Y] (ab206064)
      HRP Anti-Cytokeratin 16/K16 antibody [EP1615Y] (ab206064)

    Protocols

    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    Certificate of Compliance

    To download a Certificate of Compliance, please enter your Lot number below:

    References (0)

    Publishing research using ab206064? Please let us know so that we can cite the reference in this datasheet.

    ab206064 has not yet been referenced specifically in any publications.

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