Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- HRP Rabbit monoclonal [EP1580Y] to Cytokeratin 19
- Suitable for: IHC-P, WB
- Reacts with: Human
- Conjugation: HRP
Product nameHRP Anti-Cytokeratin 19 antibody [EP1580Y]
See all Cytokeratin 19 primary antibodies
DescriptionHRP Rabbit monoclonal [EP1580Y] to Cytokeratin 19
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse
Synthetic peptide within Human Cytokeratin 19 (C terminal). The exact sequence is proprietary.
- WB: HepG2 cytoplasmic lysate. IHC: normal human colon tissue.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark.
Dissociation constant (KD)KD = 3.70 x 10 -10 M Learn more about KD
Storage bufferpH: 7.40
Preservative: 0.1% 10% Proclin 300 Solution
Constituents: 30% Glycerol, 1% BSA, PBS
Concentration information loading...
PurityProtein A purified
- Alexa Fluor® 488 Anti-Cytokeratin 19 antibody [EP1580Y] (ab192643)
- Alexa Fluor® 647 Anti-Cytokeratin 19 antibody [EP1580Y] (ab192980)
- Anti-Cytokeratin 19 antibody [EP1580Y] - BSA and Azide free (ab195872)
- Alexa Fluor® 594 Anti-Cytokeratin 19 antibody [EP1580Y] (ab203443)
- Alexa Fluor® 555 Anti-Cytokeratin 19 antibody [EP1580Y] (ab203444)
- Alexa Fluor® 568 Anti-Cytokeratin 19 antibody [EP1580Y] (ab203445)
- APC Anti-Cytokeratin 19 antibody [EP1580Y] (ab224980)
- PE Anti-Cytokeratin 19 antibody [EP1580Y] (ab224981)
- Anti-Cytokeratin 19 antibody [EP1580Y] - Cytoskeleton Marker (ab52625)
Our Abpromise guarantee covers the use of ab193600 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||1/5000. Detects a band of approximately 44 kDa (predicted molecular weight: 44 kDa).|
FunctionInvolved in the organization of myofibers. Together with KRT8, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle.
Tissue specificityExpressed in a defined zone of basal keratinocytes in the deep outer root sheath of hair follicles. Also observed in sweat gland and mammary gland ductal and secretory cells, bile ducts, gastrointestinal tract, bladder urothelium, oral epithelia, esophagus, ectocervical epithelium (at protein level). Expressed in epidermal basal cells, in nipple epidermis and a defined region of the hair follicle. Also seen in a subset of vascular wall cells in both the veins and artery of human umbilical cord, and in umbilical cord vascular smooth muscle. Observed in muscle fibers accumulating in the costameres of myoplasm at the sarcolemma in structures that contain dystrophin and spectrin.
Sequence similaritiesBelongs to the intermediate filament family.
Developmental stagePresent in hair follicles at all stages of development.
DomainThis keratin differs from all other IF proteins in lacking the C-terminal tail domain.
- Information by UniProt
- 40 kDa keratin intermediate filament antibody
- CK 19 antibody
- CK-19 antibody
IHC image of Cytokeratin 19 staining in a section of formalin-fixed paraffin-embedded normal human colon tissue*, performed on a Leica BOND. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab193600 at 1/500 dilution, for 15 mins at room temperature. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset negative control image is taken from an identical assay without primary antibody.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
HRP Anti-Cytokeratin 19 antibody [EP1580Y] (ab193600) at 1/5000 dilution + HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate at 10 µg
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 44 kDa
Observed band size: 44 kDa
Exposure time: 5 seconds
This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab193600 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.
ab193600 has not yet been referenced specifically in any publications.