HRP Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody (ab2493)
Key features and details
- HRP Rabbit polyclonal to DDDDK tag (Binds to FLAG® tag sequence)
- Suitable for: ELISA, WB
- Reacts with: Species independent
- Conjugation: HRP
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
HRP Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody
See all DDDDK tag (Binds to FLAG® tag sequence) primary antibodies -
Description
HRP Rabbit polyclonal to DDDDK tag (Binds to FLAG® tag sequence) -
Host species
Rabbit -
Conjugation
HRP -
Tested applications
Suitable for: ELISA, WBmore details -
Species reactivity
Reacts with: Species independent -
Immunogen
Synthetic peptide corresponding to DDDDK tag (Binds to FLAG® tag sequence).
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General notes
Epitope tags provide a method to localize gene products in a variety of cell types, study the topology of proteins and protein complexes, identify associated proteins, and characterize newly identified, low abundance or poorly immunogenic proteins when protein specific antibodies are not available. Tagging with xxxDDDDK may be done at the N-terminus, N-terminus preceded by a methionine residue, C-terminus, and in internal positions of the target protein. The small size of the epitope tag and its high hydrophilicity tend to decrease the possibility of interference with protein expression, proteolytic maturation, antigenicity and function. The enterokinase cleavage site allows it to be completely removed from the purified fusion proteins.
FLAG® is a registered trade mark of Sigma Aldrich Biotechnology LP. It is used here for informational purposes only.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.19% HEPES, 0.58% Sodium chloride, 0.2% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab2493 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ELISA |
1/5000 - 1/20000.
|
|
| WB | (1) |
1/1000 - 1/10000.
|
| Notes |
|---|
|
ELISA
1/5000 - 1/20000. |
|
WB
1/1000 - 1/10000. |
Target
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Relevance
This is a useful tool for the localisation and characterisation of DDDDK tagged proteins (Binds to FLAG® tag sequence). -
Alternative names
- DDDDK epitope tag antibody
- DDDK antibody
- ddk antibody
see all
Datasheets and documents
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SDS download
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Datasheet download
References (3)
ab2493 has been referenced in 3 publications.
- Bernard PE et al. Single Amino Acid Replacements in RocA Disrupt Protein-Protein Interactions To Alter the Molecular Pathogenesis of Group A Streptococcus. Infect Immun 88:N/A (2020). PubMed: 32817331
- Kling E et al. Convergent evolution of integration site selection upstream of tRNA genes by yeast and amoeba retrotransposons. Nucleic Acids Res 46:7250-7260 (2018). PubMed: 29945249
- Belashov IA et al. Structure of HIV TAR in complex with a Lab-Evolved RRM provides insight into duplex RNA recognition and synthesis of a constrained peptide that impairs transcription. Nucleic Acids Res 46:6401-6415 (2018). PubMed: 29961805