Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- HRP Rabbit monoclonal [EPR20018-251] to DDDDK tag (Binds to FLAG® tag sequence)
- Suitable for: WB
- Reacts with: Species independent
- Conjugation: HRP
Product nameHRP Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [EPR20018-251]
See all DDDDK tag (Binds to FLAG® tag sequence) primary antibodies
DescriptionHRP Rabbit monoclonal [EPR20018-251] to DDDDK tag (Binds to FLAG® tag sequence)
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Species independent
Synthetic peptide within DDDDK tag (Binds to FLAG® tag sequence). The exact sequence is proprietary.
FLAG® is a registered trade mark of Sigma Aldrich Biotechnology LP. It is used here for informational purposes only.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Store In the Dark.
Storage bufferpH: 7.4
Preservative: 0.1% 10% Proclin 300 Solution
Constituents: 30% Glycerol, 1% BSA, PBS
Concentration information loading...
PurityProtein A purified
- Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [EPR20018-251] (ab205606)
- Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [EPR20018-251] - BSA and Azide free (ab236777)
- Alexa Fluor® 488 Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [EPR20018-251] (ab245892)
- Alexa Fluor® 647 Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [EPR20018-251] (ab245893)
Our Abpromise guarantee covers the use of ab245895 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
RelevanceThis is a useful tool for the localisation and characterisation of DDDDK tagged proteins (Binds to FLAG® tag sequence).
- DDDDK epitope tag antibody
- DDDK antibody
- ddk antibody
All lanes : HRP Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [EPR20018-251] (ab245895) at 1/1000 dilution
Lane 1 : Recombinant human RANKL protein (Active) (ab157289) cell lysate at 0.5 µg
Lane 2 : Recombinant human RANKL protein (Active) (ab157289) cell lysate at 0.1 µg
Lane 3 : Recombinant human TRAIL protein (ab157345) cell lysate at 0.5 µg
Lane 4 : Recombinant human TRAIL protein (ab157345) cell lysate at 0.1 µg
Performed under reducing conditions.
Observed band size: 35 kDa why is the actual band size different from the predicted?
Exposure time: 10 seconds
ab245895 was shown to react with DDDDK tag in western blot. Membranes were blocked in 5% milk in TBS-T (0.1% Tween®) before incubation with ab245895 overnight at 4°C at a 1 in 1000 dilution. Blots were developed with Optiblot ECL reagent (ab133456) and imaged.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab245895 has not yet been referenced specifically in any publications.