Recombinant HRP Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [EPR20018-251] (ab245895)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- HRP Rabbit monoclonal [EPR20018-251] to DDDDK tag (Binds to FLAG® tag sequence)
- Suitable for: WB
- Reacts with: Species independent
- Conjugation: HRP
Related conjugates and formulations
Overview
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Product name
HRP Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [EPR20018-251]
See all DDDDK tag (Binds to FLAG® tag sequence) primary antibodies -
Description
HRP Rabbit monoclonal [EPR20018-251] to DDDDK tag (Binds to FLAG® tag sequence) -
Host species
Rabbit -
Conjugation
HRP -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Species independent -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
FLAG® is a registered trade mark of Sigma Aldrich Biotechnology LP. It is used here for informational purposes only.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.1% Proclin 300 Solution
Constituents: 30% Glycerol (glycerin, glycerine), 1% BSA, PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR20018-251 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
- Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [EPR20018-251] (ab205606)
- Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [EPR20018-251] - BSA and Azide free (ab236777)
- Alexa Fluor® 488 Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [EPR20018-251] (ab245892)
- Alexa Fluor® 647 Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [EPR20018-251] (ab245893)
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab245895 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
1/1000.
|
Notes |
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WB
1/1000. |
Target
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Relevance
This is a useful tool for the localisation and characterisation of DDDDK tagged proteins (Binds to FLAG® tag sequence). -
Alternative names
- DDDDK epitope tag antibody
- DDDK antibody
- ddk antibody
see all
Images
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All lanes : HRP Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [EPR20018-251] (ab245895) at 1/1000 dilution
Lane 1 : Recombinant human RANKL protein (Active) (ab157289) cell lysate at 0.5 µg
Lane 2 : Recombinant human RANKL protein (Active) (ab157289) cell lysate at 0.1 µg
Lane 3 : Recombinant human TRAIL protein (ab157345) cell lysate at 0.5 µg
Lane 4 : Recombinant human TRAIL protein (ab157345) cell lysate at 0.1 µg
Performed under reducing conditions.
Observed band size: 35 kDa why is the actual band size different from the predicted?
Exposure time: 10 secondsab245895 was shown to react with DDDDK tag in western blot. Membranes were blocked in 5% milk in TBS-T (0.1% Tween®) before incubation with ab245895 overnight at 4°C at a 1 in 1000 dilution. Blots were developed with Optiblot ECL reagent (ab133456) and imaged.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab245895 has been referenced in 1 publication.
- Bhagirath AY et al. Critical cysteines in the functional interaction of adenylyl cyclase isoform 6 with Gαs. FASEB Bioadv 4:180-196 (2022). PubMed: 35664968