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  1. Link

    hrp-ddddk-tag-binds-to-flag-tag-sequence-antibody-m2-ab49763.pdf

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Tags & Cell Markers Epitope Tags DDDDK Tag
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HRP Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [M2] (ab49763)

  • Datasheet
  • SDS
Reviews (8) Submit a question References (44)

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Western blot - HRP Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [M2] (ab49763)
  • ELISA - HRP Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [M2] (ab49763)
  • Western blot - HRP Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [M2] (ab49763)

Key features and details

  • HRP Mouse monoclonal [M2] to DDDDK tag (Binds to FLAG® tag sequence)
  • Suitable for: WB, ELISA
  • Reacts with: Species independent
  • Conjugation: HRP
  • Isotype: IgG1

Overview

  • Product name

    HRP Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [M2]
    See all DDDDK tag (Binds to FLAG® tag sequence) primary antibodies
  • Description

    HRP Mouse monoclonal [M2] to DDDDK tag (Binds to FLAG® tag sequence)
  • Host species

    Mouse
  • Conjugation

    HRP
  • Tested applications

    Suitable for: WB, ELISAmore details
  • Species reactivity

    Reacts with: Species independent
  • Immunogen

    Synthetic peptide corresponding to DDDDK tag (Binds to FLAG® tag sequence).
    Sequence:

    DYKDDDDK

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • General notes

    This product was changed from ascites to tissue culture supernatant on 3rd December 2018. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

    FLAG® is a registered trade mark of Sigma Aldrich Biotechnology LP. It is used here for informational purposes only.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Lyophilized:Reconstitute to 1.0 ml with distilled deionized water vortex gently and allow vial to sit on ice for 20 minutes. Store at +4°C. Do not freeze.
  • Storage instructions

    Shipped at 4°C. Store at +4°C. Store In the Dark.
  • Storage buffer

    pH: 7.40
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 1.64% Sodium phosphate, 0.58% Sodium chloride, 1.5% BSA, 1.71% Sucrose
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    M2
  • Isotype

    IgG1
  • Research areas

    • Tags & Cell Markers
    • Epitope Tags
    • DDDDK Tag

Associated products

  • Alternative Versions

    • DyLight® 650 Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [M2] (ab117492)
    • DyLight® 550 Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [M2] (ab117495)
    • DyLight® 488 Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [M2] (ab117505)
    • PerCP Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [M2] (ab117514)
    • Europium Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [M2] (ab187914)
    • PE Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [M2] (ab72469)
    • SureLight® APC Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [M2] (ab72569)
    • Alkaline Phosphatase Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [M2] (ab81655)
  • Related Products

    • TMB ELISA Substrate (Highest Sensitivity) (ab171522)
    • TMB ELISA Substrate (High Sensitivity) (ab171523)
    • TMB ELISA Substrate (Fast Kinetic Rate) (ab171524)
    • TMB ELISA Substrate (Slow Kinetic Rate) (ab171525)
    • TMB ELISA Substrate (Slower Kinetic Rate) (ab171526)
    • TMB ELISA Substrate (Slowest Kinetic Rate) (ab171527)
    • 450 nm Stop Solution for TMB Substrate (ab171529)
    • 650 nm Stop Solution for TMB Substrate (ab171531)
    • Immunoassay Blocking Buffer (ab171534)
    • Immunoassay Blocking (BSA Free) (ab171535)

Applications

Our Abpromise guarantee covers the use of ab49763 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/10000.
ELISA 1/20000.

Target

  • Relevance

    This is a useful tool for the localisation and characterisation of DDDDK tagged proteins (Binds to FLAG® tag sequence).
  • Alternative names

    • DDDDK epitope tag antibody
    • DDDK antibody
    • ddk antibody
    • DYKDDDDK (also known as the FLAG® tag sequence) antibody
    • DYKDDDDK antibody
    • DYKDDDDK epitope tag antibody
    • DYKDDDDK tag antibody
    • ECS epitope tag antibody
    • ECS tag antibody
    • Enterokinase Cleavage Site epitope tag antibody
    • Enterokinase Cleavage Site tag antibody
    • FLAG® antibody
    • FLAG® tag antibody
    see all

Images

  • Western blot - HRP Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [M2] (ab49763)
    Western blot - HRP Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [M2] (ab49763)This image is courtesy of an anonymous Abreview
    All lanes : HRP Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [M2] (ab49763) at 1/3000 dilution

    All lanes : Saccharomyces cerevisiae Cell lysate - whole cell (S. cerevisiae cell lysate).

    Lysates/proteins at 10 µg per lane.

    Developed using the ECL technique.

    Performed under reducing conditions.

    Exposure time: 45 seconds


    Sample 1, 2, 3, 4, 5, 6 are the lysates from the strains expressing the tagged protein.

    5% milk blocking was used for 1 hour.

    See Abreview

  • ELISA - HRP Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [M2] (ab49763)
    ELISA - HRP Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [M2] (ab49763)This image is courtesy of an anonymous Abreview

    ELISA for the detection of FLAG®-tag purified protein from Escherichia coli, using ab28947 (1/5000). Please refer to abreview for further experimental details.

    Please note that in order to show a clearer image, we have brightened the original image.

    See Abreview

  • Western blot - HRP Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [M2] (ab49763)
    Western blot - HRP Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [M2] (ab49763)This image is courtesy of an anonymous Abreview

    Saccharomyces cerevisiae purified FLAG®-tag protein was detected by using ab49763 (1/1000). Please refer to abreview for further experimental details.

    See Abreview

Protocols

  • Immunocytochemistry & immunofluorescence protocols
  • Western blot protocols

Click here to view the general protocols

Datasheets and documents

    • Datasheet
    • SDS
  • References (44)

    Publishing research using ab49763? Please let us know so that we can cite the reference in this datasheet.

    ab49763 has been referenced in 44 publications.

    • Binder JL  et al. Optical induction of autophagy via Transcription factor EB (TFEB) reduces pathological tau in neurons. PLoS One 15:e0230026 (2020). PubMed: 32208437
    • Luo XB  et al. Proinflammatory Effects of Ubiquitin-Specific Protease 5 (USP5) in Rheumatoid Arthritis Fibroblast-Like Synoviocytes. Mediators Inflamm 2020:8295149 (2020). PubMed: 32214906
    • Wang X  et al. The Photorhabdus Virulence Cassettes RRSP-Like Effector Interacts With Cyclin-Dependent Kinase 1 and Causes Mitotic Defects in Mammalian Cells. Front Microbiol 11:366 (2020). PubMed: 32231646
    • Liu YL  et al. Ligustrazine reverts anthracycline chemotherapy resistance of human breast cancer by inhibiting JAK2/STAT3 signaling and decreasing fibrinogen gamma chain (FGG) expression. Am J Cancer Res 10:939-952 (2020). PubMed: 32266101
    • Kuhn A  et al. Direct ETTIN-auxin interaction controls chromatin states in gynoecium development. Elife 9:N/A (2020). PubMed: 32267233
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-8 of 8 Abreviews or Q&A

    Western blot abreview for Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [M2] (HRP)

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Saccharomyces cerevisiae Cell lysate - whole cell (S. cerevisiae cell lysate)
    Gel Running Conditions
    Reduced Denaturing (10)
    Loading amount
    10 µg
    Specification
    S. cerevisiae cell lysate
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
    Read More

    Abcam user community

    Verified customer

    Submitted Mar 04 2014

    Western blot abreview for Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [M2] (HRP)

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Purified protein (NIH3T3 cells)
    Gel Running Conditions
    Reduced Denaturing (3-8 % Tris-Acetate Gel)
    Loading amount
    5e+006 cells
    Treatment
    10 mM Sodium Butyrate for 24 hrs, 500 ug/ml Geneticin for 48 hrs
    Specification
    NIH3T3 cells
    Blocking step
    BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    Submitted Jan 07 2011

    Western blot abreview for Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [M2] (HRP)

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Escherichia coli Cell lysate - whole cell (chimeric protein)
    Gel Running Conditions
    Reduced Denaturing (4-12% bis tris)
    Loading amount
    2 µg
    Specification
    chimeric protein
    Blocking step
    Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Read More

    Abcam user community

    Verified customer

    Submitted Nov 17 2010

    Western blot abreview for Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [M2] (HRP)

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Chinese hamster Cell lysate - whole cell (CHO-S, transfected with plasmid containing insert)
    Gel Running Conditions
    Reduced Denaturing (12)
    Loading amount
    125000 cells
    Specification
    CHO-S, transfected with plasmid containing insert
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    Submitted Jun 15 2010

    Western blot abreview for Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [M2] (HRP)

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Escherichia coli Purified protein (BL21(DE3))
    Gel Running Conditions
    Reduced Denaturing (12%)
    Loading amount
    1 µg
    Specification
    BL21(DE3)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Read More

    Abcam user community

    Verified customer

    Submitted Apr 27 2010

    ELISA abreview for Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [M2] (HRP)

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    ELISA
    Sample
    Escherichia coli Purified protein (Mach1 (Invitrogen))
    Specification
    Mach1 (Invitrogen)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: RT°C
    Type
    Direct
    Read More

    Abcam user community

    Verified customer

    Submitted Feb 19 2009

    Western blot abreview for Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [M2] (HRP)

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Saccharomyces cerevisiae Purified protein (BJ5453)
    Gel Running Conditions
    Reduced Denaturing (12%)
    Loading amount
    1 µg
    Specification
    BJ5453
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Read More

    Abcam user community

    Verified customer

    Submitted Jan 14 2009

    Western blot abreview for Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [M2] (HRP)

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Escherichia coli Purified protein (Mach1)
    Gel Running Conditions
    Reduced Denaturing (12% SDS PAGE)
    Loading amount
    0.01 µg
    Specification
    Mach1
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Read More

    Abcam user community

    Verified customer

    Submitted Dec 16 2008

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