HRP Anti-DDX4 / MVH antibody [mAbcam27591] (ab196709)

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Western blot - HRP Anti-DDX4 / MVH antibody [mAbcam27591] (ab196709)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-DDX4 / MVH antibody [mAbcam27591] (ab196709)

Key features and details

  • HRP Mouse monoclonal [mAbcam27591] to DDX4 / MVH
  • Suitable for: WB, IHC-P
  • Reacts with: Mouse, Human
  • Conjugation: HRP
  • Isotype: IgG1

Overview

  • Product name

    HRP Anti-DDX4 / MVH antibody [mAbcam27591]
    See all DDX4 / MVH primary antibodies

  • Description

    HRP Mouse monoclonal [mAbcam27591] to DDX4 / MVH

  • Host species

    Mouse

  • Conjugation

    HRP

  • Tested applications

    Suitable for: WB, IHC-Pmore details

  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Common marmoset

  • Immunogen

    Synthetic peptide corresponding to Human DDX4/ MVH aa 700 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin.

  • Positive control

    • WB: Mouse testis tissue lysate. IHC-P: Normal human testis tissue.

  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Applications

Our Abpromise guarantee covers the use of ab196709 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/8000. Detects a band of approximately 80 kDa (predicted molecular weight: 76 kDa).
IHC-P 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    Probable ATP-dependent RNA helicase required during spermatogenesis (PubMed:10920202, PubMed:21034600). Required to repress transposable elements and preventing their mobilization, which is essential for the germline integrity. Acts via the piRNA metabolic process, which mediates the repression of transposable elements during meiosis by forming complexes composed of piRNAs and Piwi proteins and governs the methylation and subsequent repression of transposons. Involved in the secondary piRNAs metabolic process, the production of piRNAs in fetal male germ cells through a ping-pong amplification cycle.

  • Tissue specificity

    Expressed only in ovary and testis. Expressed in migratory primordial germ cells in the region of the gonadal ridge in both sexes.

  • Sequence similarities

    Belongs to the DEAD box helicase family. DDX4/VASA subfamily.
    Contains 1 helicase ATP-binding domain.
    Contains 1 helicase C-terminal domain.

  • Cellular localization

    Cytoplasm. Cytoplasm, perinuclear region. Component of the meiotic nuage, also named P granule, a germ-cell-specific organelle required to repress transposon activity during meiosis.
  • Information by UniProt

  • Database links

    • Alternative names

      • DDX 4 antibody
      • Ddx4 antibody
      • DDX4_HUMAN antibody
      • DEAD (Asp Glu Ala Asp) box polypeptide 4 antibody
      • DEAD box protein 4 antibody
      • DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 4 antibody
      • MVH antibody
      • Probable ATP dependent RNA helicase DDX4 antibody
      • Probable ATP-dependent RNA helicase DDX4 antibody
      • VASA antibody
      • Vasa homolog antibody
      see all

    Images

    • Western blot - HRP Anti-DDX4 / MVH antibody [mAbcam27591] (ab196709)
      Western blot - HRP Anti-DDX4 / MVH antibody [mAbcam27591] (ab196709)
      HRP Anti-DDX4 / MVH antibody [mAbcam27591] (ab196709) at 1/8000 dilution + Testis (Mouse) Tissue Lysate at 20 µg

      Developed using the ECL technique.

      Performed under reducing conditions.

      Predicted band size: 76 kDa
      Observed band size: 79 kDa
      why is the actual band size different from the predicted?


      Exposure time: 20 seconds


      This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab196709 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-DDX4 / MVH antibody [mAbcam27591] (ab196709)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-DDX4 / MVH antibody [mAbcam27591] (ab196709)

      IHC image of DDX4 / MVH staining in a section of formalin-fixed paraffin-embedded normal human testis*, performed on a Leica BOND. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab196709 at 1/500 dilution, for 15 mins at room temperature. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset negative control image is taken from an identical assay without primary antibody.

      For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

      *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre.

    References (0)

    Publishing research using ab196709? Please let us know so that we can cite the reference in this datasheet.

    ab196709 has not yet been referenced specifically in any publications.

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