Recombinant HRP Anti-delta 1 Catenin/CAS antibody [YE372] (ab202914)
![Western blot - HRP Anti-delta 1 Catenin/CAS antibody [YE372] (ab202914)](https://www.abcam.com/ps/products/202/ab202914/Images/ab202914-252315-WBab2029141.jpg "Western blot - HRP Anti-delta 1 Catenin/CAS antibody [YE372] (ab202914)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- HRP Rabbit monoclonal [YE372] to delta 1 Catenin/CAS
- Suitable for: WB
- Reacts with: Human
- Conjugation: HRP
Overview
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Product name
HRP Anti-delta 1 Catenin/CAS antibody [YE372]
See all delta 1 Catenin/CAS primary antibodies -
Description
HRP Rabbit monoclonal [YE372] to delta 1 Catenin/CAS -
Host species
Rabbit -
Conjugation
HRP -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human delta 1 Catenin/CAS (N terminal). The exact sequence is proprietary. Before aa101 of Human delta 1 Catenin (isoform 1ABC, 968AA).
Database link: O60716 -
Positive control
- WB: HeLa whole cell lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.1% 10% Proclin 300 Solution
Constituents: 30% Glycerol, 1% BSA, PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
YE372 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Related Products
Applications
Our Abpromise guarantee covers the use of ab202914 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/5000. Detects a band of approximately 120 kDa (predicted molecular weight: 108 kDa). |
Target
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Function
Binds to and inhibits the transcriptional repressor ZBTB33, which may lead to activation of target genes of the Wnt signaling pathway (By similarity). May associate with and regulate the cell adhesion properties of both C- and E-cadherins. Implicated both in cell transformation by SRC and in ligand-induced receptor signaling through the EGF, PDGF, CSF-1 and ERBB2 receptors. Promotes GLIS2 C-terminal cleavage. -
Tissue specificity
Expressed in vascular endothelium. -
Sequence similarities
Belongs to the beta-catenin family.
Contains 10 ARM repeats. -
Domain
A possible nuclear localization signal exists in all isoforms where Asp-626--631-Arg are deleted. -
Post-translational
modificationsPhosphorylated by protein-tyrosine kinases. Dephosphorylated by PTPRJ. -
Cellular localization
Cytoplasm. Nucleus. Cell membrane. Interaction with GLIS2 promotes nuclear translocation (By similarity). NANOS1 induces its translocation from sites of cell-cell contact to the cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 1500 Human
- Omim: 601045 Human
- SwissProt: O60716 Human
- Unigene: 166011 Human
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Alternative names
- Cadherin associated Src substrate antibody
- Cadherin-associated Src substrate antibody
- CAS antibody
see all
Images
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Western blot - HRP Anti-delta 1 Catenin/CAS antibody [YE372] (ab202914)HRP Anti-delta 1 Catenin/CAS antibody [YE372] (ab202914) at 1/5000 dilution +
HeLa whole cell lysate (ab150035) at 10 µg
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 108 kDa
Observed band size: 120 kDa why is the actual band size different from the predicted?
Exposure time: 1 minuteThis blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab202914 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.
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![HRP Anti-delta 1 Catenin/CAS antibody [YE372] (ab202914)](https://www.abcam.com/ps/products/202/ab202914/Images/ab202914-3-benefits-of-recombinant-antibodies.png)
HRP Anti-delta 1 Catenin/CAS antibody [YE372] (ab202914)
References (0)
ab202914 has not yet been referenced specifically in any publications.
