HRP Anti-Digoxigenin antibody [HRP.21H8] (ab6212)

Reviews (4)Q&A (13)References (20)
Immunohistochemistry (Frozen sections) - HRP Anti-Digoxigenin antibody [HRP.21H8] (ab6212)

    Key features and details

    • HRP Mouse monoclonal [HRP.21H8] to Digoxigenin
    • Suitable for: WB, ELISA, IHC-P, IHC-Fr
    • Reacts with: Species independent
    • Conjugation: HRP
    • Isotype: IgG1

    Overview

    • Product name

      HRP Anti-Digoxigenin antibody [HRP.21H8]
      See all Digoxigenin primary antibodies

    • Description

      HRP Mouse monoclonal [HRP.21H8] to Digoxigenin

    • Host species

      Mouse

    • Conjugation

      HRP

    • Tested applications

      Suitable for: WB, ELISA, IHC-P, IHC-Frmore details

    • Species reactivity

      Reacts with: Species independent

    • Immunogen

      Chemical/ Small Molecule conjugated to bovine serum albumin.

    • General notes

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    Properties

    • Form

      Liquid

    • Storage instructions

      Shipped at 4°C. Store at +4°C.

    • Storage buffer

      pH: 7.40
      Preservative: 0.01% Thimerosal (merthiolate)
      Constituents: PBS, 50% Glycerol, 1% BSA
    • Concentration information loading...

    • Purity

      Protein A purified

    • Clonality

      Monoclonal

    • Clone number

      HRP.21H8

    • Myeloma

      unknown

    • Isotype

      IgG1

    • Light chain type

      kappa

    • Research areas

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab6212 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    WB
    1/500 - 1/5000.
    ELISA
    1/1000 - 1/10000.
    IHC-P (1)
    1/200 - 1/2000. Use in combination with tyramide signal amplification.
    IHC-Fr (2)
    1/200 - 1/2000. 1/200 - 1/2000. Use in combination with tyramide signal amplification.
    Notes
    WB
    1/500 - 1/5000.
    ELISA
    1/1000 - 1/10000.
    IHC-P
    1/200 - 1/2000. Use in combination with tyramide signal amplification.
    IHC-Fr
    1/200 - 1/2000. 1/200 - 1/2000. Use in combination with tyramide signal amplification.

    Target

    • Relevance

      Digoxigenin (DIG) is a steroid found exclusively in the flowers and leaves of the plants Digitalis purpurea and Digitalis lanata. Digoxigenin is chemically closely related to Digoxin, the cardiac glycoside used for the treatment of various heart diseases. The term 'genin' at the end of Digoxigenin, refers to only the aglycone portion (without the sugar) part of the molecule,thus Digoxigenin is the steroid component of Digoxin, - minus the (digitose) sugar residues. DIG can be covalently added to proteins or nucleic acids which makes it very useful in diverse applications.

    • Alternative names

      • 1672-46-4 antibody
      • BRN 0096479 antibody
      • DIG antibody
      • EINECS 216-806-2 antibody
      • HSDB 7108 antibody
      • Lanadigenin antibody
      • Lanadigigenin antibody
      • ST056392 antibody
      see all

    Images

    • Immunohistochemistry (Frozen sections) - HRP Anti-Digoxigenin antibody [HRP.21H8] (ab6212)
      Immunohistochemistry (Frozen sections) - HRP Anti-Digoxigenin antibody [HRP.21H8] (ab6212)

      ab6212 at a dilution of 1/1000 staining Digoxigenin from human pancreatic tissue sections by immunohistochemistry (frozen). The antibody was incubated with the tissue for 2 hours.

      This image is courtesy of an Abreview submitted by an anonymous researcher on 9 December 2005.

      See Abreview

    References (20)

    Publishing research using ab6212? Please let us know so that we can cite the reference in this datasheet.

    ab6212 has been referenced in 20 publications.

    • Zhang Y  et al. Extracellular vesicle-encapsulated microRNA-23a from dorsal root ganglia neurons binds to A20 and promotes inflammatory macrophage polarization following peripheral nerve injury. Aging (Albany NY) 13:6752-6764 (2021). PubMed: 33621204
    • Wang R  et al. microRNA-210/ Long non-coding RNA MEG3 axis inhibits trophoblast cell migration and invasion by suppressing EMT process. Placenta 109:64-71 (2021). PubMed: 33990028
    • Li MK  et al. Long non-coding RNA MEG3 suppresses epithelial-to-mesenchymal transition by inhibiting the PSAT1-dependent GSK-3ß/Snail signaling pathway in esophageal squamous cell carcinoma. Oncol Rep 44:2130-2142 (2020). PubMed: 32901893
    • Shao G  et al. lncRNA CASC9 positively regulates CHK1 to promote breast cancer cell proliferation and survival through sponging the miR-195/497 cluster. Int J Oncol 54:1665-1675 (2019). PubMed: 30816435
    • Filer JE  et al. A Nuclease Protection ELISA Assay for Colorimetric and Electrochemical Detection of Nucleic Acids. Anal Methods 11:1027-1034 (2019). PubMed: 31656535
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
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    1-10 of 17 Abreviews or Q&A

    Other (Northern Blot) abreview for Anti-Digoxigenin antibody [HRP.21H8] (HRP)

     Good
    Abreviews
    abreview image
    Application
    Other - Do not use
    Sample
    Influenza A Cell lysate - other (in vitro RNA transcription for nothern blot)
    Read More

    Abcam user community

    Verified customer

    Submitted Feb 15 2019

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Digoxigenin antibody [HRP.21H8] (HRP)

     Excellent
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (Pancreas)
    Specification
    Pancreas
    Fixative
    Paraformaldehyde
    Antigen retrieval step
    None
    Permeabilization
    No
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C
    Read More

    Abcam user community

    Verified customer

    Submitted Jun 01 2011

    Immunohistochemistry (Frozen sections) abreview for Anti-Digoxigenin antibody [HRP.21H8] (HRP)

     Good
    Abreviews
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Rat Tissue sections (brain)
    Specification
    brain
    Fixative
    Paraformaldehyde
    Permeabilization
    No
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 20% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    Submitted Mar 31 2009

    Immunohistochemistry (Frozen sections) abreview for Anti-Digoxigenin antibody [HRP.21H8] (HRP)

     Good
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Human Tissue sections (in situ hybridization in pancreas)
    Specification
    in situ hybridization in pancreas
    Fixative
    Paraformaldehyde
    Blocking step
    Other as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1
    Read More

    Abcam user community

    Verified customer

    Submitted Dec 09 2005

    Question

    I would like a certificate of compliance for the following product: Catalog # ab6212 Anti-Digoxigenin antibody [HRP.21H8] (HRP) Lot # GR63837-5 Thank you!

    Read More
    Answer

    Thank you for contacting us.

    I have attached the CoC for ab6212 to this email.

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Use our products? Submit an Abreview. Earn rewards!
    https://www.abcam.com/abreviews

    Read More

    Question

    Thank you for your reply.

    I have received the data from this customer.

    Attached please find the protocol (sheet1), results(sheet2) and modified ELISA schematic (tif)fromthis customer.

    Would you please help this customer check the results?

    Read More
    Answer

    Thanks so much for sending the protocol, data and schematic image. That is all very helpful to see.

    From previous email, it was noted that lot GR40978-1 produced a linear curve while lots GR44124-3, GF63837-3 and GR63837-4 produced a plateau instead. Comparing the data provided using these different lots I am not able to see a significant difference between the raw OD readings from these different lots. All curves seem consistant, showing a minimum detection limit of about 1000ng/ml

    Read More

    Question

    Thank you for your reply.
    Our customer recently has ordered ab6212 with lot# GR62763-3 and GR62763-4 (requesting 2 different lot when putting the order).
    Shehad usedbothlot# gr40978-1 (two vials, good results) andlot#Gr44124-3 (two vial, unidealresults and data different from lot# gr40978-1) in ELISA application. In order to confirm her experimental data, she would like to obtain two different lot of ab612 to reconduct. Therefore, she would like to confirm thatwhether these two lotsare the same batch of manufacture (she wants 2 independent batch)? How many stock with lot# GR62763-3 and GR62763-4? If the experiment set up ready, she calims that 10 vials may be needed.

    Read More
    Answer

    Thank you for your enquiry.

    Regarding lots GR62763-3 and GR62763-4 for ab6212: These are treated as 2 different lots in our stock system. However we receive this antibody from an outside lab and, from this lab, GR62763-3 and GR62763-4 are not produced in separate production runs. They are part of the same production run.


    I hope this helps. Please contact me again if you have any further questions.

    Read More

    Question

    ab6212 (Mouse monoclonal [HRP.21H8] to Digoxigenin (HRP)) What is the concentration of this antibody?

    Read More
    Answer

    Thank you for your patience in this matter. I have received a reply from the originator of ab6212. The concentration of this product is lot dependent, usually between 0.2 - 0.5 mg/ml. The concentration of our present lot is ~0.4 mg/ml. Please don't hesitate to contact us again if you have further questions.

    Read More

    Question

    What concentration of antibody was used in In Situ Hybridization?

    Read More
    Answer

    Thank you for your enquiry. I have received word from the originator that they have not specifically tested this product in in situ hybridization. Please see their response below: This product was used successfully in IHC at the dilution of 1/1000. For in situ hybridization, we don't have any results, however we assume that many customers used it for this application. We recommend the dilution of 1/500 to 1/1000. I have removed ISH from the datasheet as a tested application; however, there doesn't seem to be any reason it won't work. Please let me know if you have any problems.

    Read More

    Question

    ab6212 = Digoxigenin antibody [HRP.21H8] Ab concentration? Immunogen and specificity of Ab? Need to contact originator of ab6212.

    Read More
    Answer

    Thank you for your patience. Regarding ab6212 (Digoxigenin antibody [HRP.21H8]), here is the requested information: antibody concentration: 0.3 mg/ml immunogen: digoxigenin coupled to KLH (details unavailable) I hope this information helps. Please don't hesitate to contact me again if you have further questions.

    Read More

    1-10 of 17 Abreviews or Q&A

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