HRP Anti-Digoxigenin antibody [HRP.21H8] (ab6212)
Key features and details
- HRP Mouse monoclonal [HRP.21H8] to Digoxigenin
- Suitable for: IHC-Fr
- Reacts with: Species independent
- Conjugation: HRP
- Isotype: IgG1
Overview
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Product name
HRP Anti-Digoxigenin antibody [HRP.21H8]
See all Digoxigenin primary antibodies -
Description
HRP Mouse monoclonal [HRP.21H8] to Digoxigenin -
Host species
Mouse -
Conjugation
HRP -
Tested applications
Suitable for: IHC-Frmore details -
Species reactivity
Reacts with: Species independent -
Immunogen
Chemical/ Small Molecule conjugated to bovine serum albumin.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.40
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: PBS, 50% Glycerol, 1% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
HRP.21H8 -
Myeloma
unknown -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Associated products
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Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab6212 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
IHC-Fr | (2) |
1/200 - 1/2000.
1/200 - 1/2000. Use in combination with tyramide signal amplification. |
Notes |
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IHC-Fr
1/200 - 1/2000. 1/200 - 1/2000. Use in combination with tyramide signal amplification. |
Target
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Relevance
Digoxigenin (DIG) is a steroid found exclusively in the flowers and leaves of the plants Digitalis purpurea and Digitalis lanata. Digoxigenin is chemically closely related to Digoxin, the cardiac glycoside used for the treatment of various heart diseases. The term 'genin' at the end of Digoxigenin, refers to only the aglycone portion (without the sugar) part of the molecule,thus Digoxigenin is the steroid component of Digoxin, - minus the (digitose) sugar residues. DIG can be covalently added to proteins or nucleic acids which makes it very useful in diverse applications. -
Alternative names
- 1672-46-4 antibody
- BRN 0096479 antibody
- DIG antibody
see all
Images
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ab6212 at a dilution of 1/1000 staining Digoxigenin from human pancreatic tissue sections by immunohistochemistry (frozen). The antibody was incubated with the tissue for 2 hours.
This image is courtesy of an Abreview submitted by an anonymous researcher on 9 December 2005.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (22)
ab6212 has been referenced in 22 publications.
- Zhang WY et al. Long noncoding RNA Gas5 induces cell apoptosis and inhibits tumor growth via activating the CHOP-dependent endoplasmic reticulum stress pathway in human hepatoblastoma HepG2 cells. J Cell Biochem 123:231-247 (2022). PubMed: 34636091
- Zhang Y et al. Extracellular vesicle-encapsulated microRNA-23a from dorsal root ganglia neurons binds to A20 and promotes inflammatory macrophage polarization following peripheral nerve injury. Aging (Albany NY) 13:6752-6764 (2021). PubMed: 33621204
- Wang R et al. microRNA-210/ Long non-coding RNA MEG3 axis inhibits trophoblast cell migration and invasion by suppressing EMT process. Placenta 109:64-71 (2021). PubMed: 33990028
- Dos Santos Á et al. High-throughput mechanobiology: Force modulation of ensemble biochemical and cell-based assays. Biophys J 120:631-641 (2021). PubMed: 33453266
- Li MK et al. Long non-coding RNA MEG3 suppresses epithelial-to-mesenchymal transition by inhibiting the PSAT1-dependent GSK-3ß/Snail signaling pathway in esophageal squamous cell carcinoma. Oncol Rep 44:2130-2142 (2020). PubMed: 32901893