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  1. Link

    hrp-e-coli-antibody-ab68450.pdf

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Microbiology Organism Bacteria Gram negative Bacteria Escherichia coli
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HRP Anti-E. coli antibody (ab68450)

  • Datasheet
  • SDS
Reviews (1)Q&A (3)References (1)

Product price, shipping and contact information

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Key features and details

  • HRP Rabbit polyclonal to E. coli
  • Suitable for: ELISA
  • Reacts with: Escherichia coli
  • Conjugation: HRP
  • Isotype: IgG

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Overview

  • Product name

    HRP Anti-E. coli antibody
    See all E. coli primary antibodies
  • Description

    HRP Rabbit polyclonal to E. coli
  • Host species

    Rabbit
  • Conjugation

    HRP
  • Tested applications

    Suitable for: ELISAmore details
  • Species reactivity

    Reacts with: Escherichia coli
  • Immunogen

    Tissue, cells or virus corresponding to Escherichia coli E. coli.

  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C.
  • Storage buffer

    Preservative: 0.002% Thimerosal (merthiolate)
    Constituents: 1% BSA, PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Microbiology
    • Organism
    • Bacteria
    • Gram negative Bacteria
    • Escherichia coli

Associated products

  • Related Products

    • TMB ELISA Substrate (Highest Sensitivity) (ab171522)
    • TMB ELISA Substrate (High Sensitivity) (ab171523)
    • TMB ELISA Substrate (Fast Kinetic Rate) (ab171524)
    • TMB ELISA Substrate (Slow Kinetic Rate) (ab171525)
    • TMB ELISA Substrate (Slower Kinetic Rate) (ab171526)
    • TMB ELISA Substrate (Slowest Kinetic Rate) (ab171527)
    • 450 nm Stop Solution for TMB Substrate (ab171529)
    • 650 nm Stop Solution for TMB Substrate (ab171531)
    • Immunoassay Blocking Buffer (ab171534)
    • Immunoassay Blocking (BSA Free) (ab171535)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab68450 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA (1)
1/200.
Notes
ELISA
1/200.

Target

  • Relevance

    Escherichia coli is a gram negative bacillus that belongs to a larger group of Enterobacteriae - bacteria that inhabit the gastrointestinal tract. Although usually a harmless resident of the gut, some strains have the potential to cause serious problems, especially where there is an immature immune system or immunosuppression, or where the subtype of organism has acquired the ability to produce pathogenic toxins.
  • Alternative names

    • Escherichia coli antibody

Protocols

  • Immunohistochemistry protocols
  • Western blot protocols

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (1)

Publishing research using ab68450? Please let us know so that we can cite the reference in this datasheet.

ab68450 has been referenced in 1 publication.

  • Wang C  et al. Detection of extremely low concentration waterborne pathogen using a multiplexing self-referencing SERS microfluidic biosensor. J Biol Eng 11:9 (2017). PubMed: 28289439

Customer reviews and Q&As

Show All Reviews Q&A
Submit a question

1-3 of 3 Q&A

Question

How is the immunogen for this antibody prepared: whole, intact E. coli, or lysate?

Read More

Abcam community

Verified customer

Asked on Dec 05 2014

Answer

The immungen is a mixture of intact and lysed bacteria of various E. coli strains, so the antibody will likely detect surface antigens in addition to intracellular antigens.

Read More

Tom Ruyle

Abcam Scientific Support

Answered on Dec 05 2014

Question

Thanks a lot

One quick additional question. I have read that Strep uberis can be picked up by Group B antibodies. What do you say to that?

Kind Regards

Read More

Abcam community

Verified customer

Asked on Dec 10 2012

Answer

Thank you for contacting us.

I have attached the list of antibodies we have along with details. Please check.

I am sorry I am unable to confirm the species cross reactivity of the these antibodies other than waht is given on the datasheet.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Free Rabbit monoclonal antibody with any purchase of a primary antibody, while stocks last! Quote “RABMAB-XBSMG” in your next primary antibody order. For more information, visit the following link: https://www.abcam.com/index.html?pageconfig=resource&rid=15447

Read More

Abcam Scientific Support

Answered on Dec 10 2012

Question

We are developing an assay for agricultural diagnostics and we are interested in following antibodies.
Staphylococcus aureus,
Strep. dysgalactiae,
Strep. uberis,
Enterococci generic
Strep agalactiae
Esherichia coli,
Klebsiella generic

I saw your Staphylococcus aureus product online and was wondering if you had more antibodies to offer against all or a subset of the bacteria listed above.

Kind Regards

Read More

Abcam community

Verified customer

Asked on Dec 10 2012

Answer

Thank you for contacting us.

ab41181 is anti Strep agalactiae, it will weakly cross react with Strep. dysgalactiae.

ab41203 is anti Strep agalactiae, it will weakly cross react with Strep. dysgalactiae.

ab19980 is an anti Enterococcus antibody

ab68450, ab69185 and ab68451 are anti E. Coli antibodies.

ab69468, ab20947, ab69467 and ab65926 are the Klebsiella spp specific antibodies.

We do not have anti Strep. uberis antibodies.

Please check our new western blot range:

https://www.abcam.com/index.html?c=5634

https://www.abcam.com/index.html?pageconfig=productmap&cl=918

https://www.abcam.com/index.html?pageconfig=resource&rid=12886

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Free Rabbit monoclonal antibody with any purchase of a primary antibody, while stocks last! Quote “RABMAB-XBSMG” in your next primary antibody order. For more information, visit the following link: https://www.abcam.com/index.html?pageconfig=resource&rid=15447

Read More

Abcam Scientific Support

Answered on Dec 10 2012

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