HRP Anti-E. coli O + E. coli K antibody (ab21110)

Submit a review Q&A (6)

Key features and details

  • HRP Goat polyclonal to E. coli O + E. coli K
  • Suitable for: ELISA, ICC/IF
  • Reacts with: Escherichia coli
  • Conjugation: HRP
  • Isotype: IgG

Overview

  • Product name

    HRP Anti-E. coli O + E. coli K antibody
    See all E. coli O + E. coli K primary antibodies

  • Description

    HRP Goat polyclonal to E. coli O + E. coli K

  • Host species

    Goat

  • Conjugation

    HRP

  • Tested applications

    Suitable for: ELISA, ICC/IFmore details

  • Species reactivity

    Reacts with: Escherichia coli

  • Immunogen

    Tissue, cells or virus corresponding to Escherichia coli E. coli O + E. coli K. Mixture of E. coli serotypes.

  • General notes

    The antibody is covalently coupled to a highly purified preparation of horseradish peroxidase (RZ>3). Care is taken to ensure adequate conjugation while preserving maximum enzyme activity. Free enzyme is absent.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab21110 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA
Use at an assay dependent dilution.
ICC/IF
Use at an assay dependent dilution.
Notes
ELISA
Use at an assay dependent dilution.
ICC/IF
Use at an assay dependent dilution.

Target

  • Relevance

    Escherichia coli (E. coli) are Gram negative enteric bacteria that live in the human gut. Its presence in nature is an indication of human feacal pollution. E. coli also causes human disease such as urinary tract infections and neonatal meningitis. Infection with particular enterotoxigenic strains can lead to life threatening intestinal diseases. E. coli are distinguished immunologically by serotyping based on three types of antigen: the somatic or O antigen which corresponds to terminal sugars on the cell surface lipopolysaccharide, the capsular or K antigens and the flagellar or H antigens.

  • Alternative names

    • Capsular antigen antibody
    • Escherichia coli antibody
    • Escherichia coli K antibody
    • Escherichia coli O antibody
    • K antigen antibody
    • O antigen antibody
    see all

References (0)

Publishing research using ab21110? Please let us know so that we can cite the reference in this datasheet.

ab21110 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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1-6 of 6 Abreviews or Q&A

Question

Ok. I will look for XXXXXXXXXX.

Thank you.

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Answer

Thank you for your email.

Could you let me know which antibody you would like to try? We have following abs available;

ab33837

ab31499

Thanks!

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Question

Regarding your advice about the direct-ELISA assay, I performed directa- ELISA for E.coli (DH5a strain) detection. I also performed the same experiment with Listeria monocytogenes using Anti-Listeria monocytogenes antibody [LZF7] (HRP) ab 24456 and also with Salmonella enterica using ab20771 Rabbit polyclonal to Salmonella (HRP). All the experiments were performing following your advice.
In the attachment you may find a document word with the results that I´ve obtained. There is no recognition from the antibodies specific for both E.coli and Listeria. I would like for this reason to inquire for the replacement of these antibodies.
Best regards,

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Answer

Thank you for your email. I have taken over this case from XXXXX so I will be dealing with this case now on.

The protocol recommended by XXXXXXX is only valid for ab21110. The protocol for ab20771 is available online, please follow that to get the results.

From the results you have kindly sent I believe that perhaps the antibody have gone off during storage. The Abcam product guarantee applies to product when the complaints are raised within 6 month time which unfortunately was not the case for this product however we acknowledge the time you have spent in laboratory so we are happy making an exception this time and would like to offer you a replacement vial of ab21110.

Could you confirm if you are interested in receiving the same antibody or a different one?

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Question

Regarding the antibody number ab 21110, the direct ELISA was performed by directly adsorbing the E.coli on the wells and this antibody was added after the blocking and washing steps. Troubleshooting was made increasing antibody concentrations and different E.coli concentrations.
We bought several antibodies from you which includes anti-Salmonella, anti-Listeria and anti-Ec.oli and also the corresponding peroxidase labeled antibodies all at the same time. When we received them we took aliquots and store properly on a freezer. We have started to work with salmonella system and it is only now that we are starting with E.coli and Listeria systems. We have not been expecting these problems because we have used your salmonella antibodies for several times which gave us enough basis to purchase the other antibodies ahead of time.
In the attachment you may find the results obtained for the direct ELISA and also the protocol that I had followed based from your website.
I am looking forward for a favorable response and for your usual costumer support.
Best regards

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Answer

Thank you for your patience.

My colleague is out of office today but I wish to pass some useful information to you.

Whole bacteria do not absorb well when coated wet in ELISA wells. You may need to add different dilutions of the bacterial suspension and then let them dry down in the wells. This can be accomplished by letting the plate sit out overnight or by placing the plate in an incubator that has a fan function to circulate the air (also at room temp). When dry, the wells should then be washed well with a detergent buffer to remove any material that is not firmly fixed by drying.

We hope this will be useful for you.

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Question

Dear Sir/ Madam,
Below, you may find the abcam questionaire for the antibodyab 21110.
1) Abcam product code ab ab21110
2) Abcam order reference number or product batch number: 988211

3) Description of the problem: No signal; The antibody does not detect adsorbed E.coli

4) Type of ELISA: direct

5) Sample details: E.coli in BHI medium

6) Target antigen (if found in heterogeneous sample)

7) Positive control: N/A
8) Negative control: BHI medium

9) Blocking agent (eg BSA…): BSA
Concentration: 3%
Blocking time: 2h
Blocking temperature: RT

10) Capture or Primary antibody information: N/A
Used to coat wells? Yes/no
Species
Reacts against
Concentration or dilution
Diluent buffer
Incubation time
Incubation temperature

11)Detection antibody: ab21110
Species: Goat
Reacts against: many O and K antigenic serotypes of Escherichia coli.
Concentration or dilution: 1/1000 and 1/2000
Diluent buffer : PBST with 3% BSA
Incubation time: 1h
Incubation temperature: RT
Conjugation: HRP conjugated



12) Enzyme labelled secondary antibody: N/A
Species
Reacts against
Concentration or dilution
Diluent buffer
Incubation time
Incubation temperature:
Conjugation

13) Washing after primary and secondary antibodies:
Buffer: PBST with BSA 3%
Number of washes: 2
Length of washes: 5 min

14) Substrate: TMB
Incubation time: 30 min

15) How many times have you run this ELISA? 4 times in triplicates
Do you obtain the same results every time? yes
What steps have you altered to try and optimize the use of this antibody? Increase the antibody concentration and compared with different concentrations of E.coli.

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Answer

Thank you for your email. I am sorry to hear that you have been experiencing problems with this antibody.

I have read the details you have kindly provided and have following further questions for better understanding of the problem;

- how exactly the antibody was used? Did you coat E.Coli sample to wells and then added antibody?
- Have you done any troubleshooting of protocol?
- Could you share ELISA results with us?
- The ab was purchased in Dec 2011; how it was stored until first use?
- How the E.Coli samples were prepared?
- Could you provide step by step followed protocol?

Abpromise guarantee applies to products if the problem is reported within 6 month from purchase. Could you explain why there was a delay in reporting for this complaint?

Thank you very much for your cooperation. I will look forward to hearing from you soon.

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Question

Thank you for your response! Very helpful.

I think I’ll have to test for enterobacteriaceae cross-reactivity myself.

Best regards

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Answer

I'm glad you found the information of help.

If you do test any of these antibodies we would be very interested to hear of the results, regardless of if they are positive or not. We like to encourage all of our customers to submit an Abreview via the online product datasheet with any data they collect with our antibodies. As a reward for doing this, each Abreview earns Abpoints that can be used for discounts on future purchases or rewards such as Amazon.com gift certificates.

To find out more about our Abreview system, please see the following link:

https://www.abcam.com/abreviews

If you let me know which antibody in particular you are interested in using I may be able to provide you with a testing discount code. More information on this scheme can be found form the following link:

www.abcam.com/collaborationdiscount

I hope this information has been of help. If you have any further questions, please do not hesitate to ask.

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Question

Phone call requesting information on if any of the anti-E. coli antibodies have been tested for their specificity.

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Answer

Thank you for contacting us and your interest in our products.

I can now share some more information relating to the antibodies we have directed towards E. coli (O and K). We have two different antibodies directed against this bacterium, with different conjugation available:

ab31499, unlabelled(ab33604, FITC; ab33837, HRP; ab33839, Biotin conjugated of same antibody preparation as ab31499)

The immunogen for these antibodies is a mixture of the following E. coli strains: O157:H7, O20, O125, O55, O111 & K12. The mixture of these strains was of intact plus lysed bacteria which had not been denatured in any way. All E. coli have many surface and internal antigens in common and this antibody recognizes this large mixture and therefore reacts with most, if not all, E. coli. Theseantibodies have been tested against the strains listed above but have not been cross-absorbed and may react with related Enterobacteriaceae. These potential cross-reacting strains have not been identified.

ab20765, ab20856 and ab21110 are Biotin, FITC and HRP conjugates of the same antibody.

These antibodies have been raised against a mixture of E. coli serotypes.These antibodies have been tested for reactivity against the immunogen strains of E.coli but again they have not been absorbed and will most likely cross react withother related Enterobacteriaceae.



I am sorry that in this case I was not able to be of more help. If you have any further questions, please do not hesitate to contact us again.

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