HRP Anti-E tag antibody (ab19400)

Submit a review Q&A (1)References (5)
Western blot - HRP Anti-E tag antibody (ab19400)

    Key features and details

    • HRP Goat polyclonal to E tag
    • Suitable for: ICC, ELISA, WB
    • Reacts with: Species independent
    • Conjugation: HRP
    • Isotype: IgG

    Overview

    • Product name

      HRP Anti-E tag antibody
      See all E tag primary antibodies

    • Description

      HRP Goat polyclonal to E tag

    • Host species

      Goat

    • Conjugation

      HRP

    • Tested applications

      Suitable for: ICC, ELISA, WBmore details

    • Species reactivity

      Reacts with: Species independent

    • Immunogen

      Synthetic peptide:

      GAPVPYPDPLEPR

      (E tag) conjugated to KLH.
      Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

    • General notes

      Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

      Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

      We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

      In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

      We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

      Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

      Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

    Properties

    • Form

      Liquid

    • Storage instructions

      Shipped at 4°C. Store at +4°C.

    • Storage buffer

      pH: 6.8
      Preservative: 0.01% Thimerosal (merthiolate)
      Constituents: 1.19% HEPES, 0.58% Sodium chloride, 0.2% BSA
    • Concentration information loading...

    • Purity

      Immunogen affinity purified

    • Clonality

      Polyclonal

    • Isotype

      IgG

    • Research areas

    Applications

    Our Abpromise guarantee covers the use of ab19400 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    ICC 1/200 - 1/500.
    ELISA 1/10000 - 1/100000.
    WB 1/1000 - 1/10000. Predicted molecular weight: 1 kDa. Colorimetric: 1/1000 - 1/10000. Chemiluminesence: 1/1000 - 1/30000.

    Target

    • Relevance

      E tag is a peptide epitope tag, sequence GAPVPYPDPLEPR.

    • Alternative names

      • E epitope tag antibody
      • GAPVPYPDPLEPR epitope tag antibody
      • GAPVPYPDPLEPR tag antibody

    Images

    • Western blot - HRP Anti-E tag antibody (ab19400)
      Western blot - HRP Anti-E tag antibody (ab19400)
      All lanes : HRP Anti-E tag antibody (ab19400) at 0.04 µg/ml

      Lane 1 : E. coli whole cell lysate expressing a multi-tag fusion protein at 0.2 µg
      Lane 2 : E. coli whole cell lysate expressing a multi-tag fusion protein at 0.1 µg
      Lane 3 : E. coli whole cell lysate expressing a multi-tag fusion protein at 0.05 µg

      Predicted band size: 1 kDa


      Exposure time: 3 seconds

    References (5)

    Publishing research using ab19400? Please let us know so that we can cite the reference in this datasheet.

    ab19400 has been referenced in 5 publications.

    • Cho A  et al. Single-Cell Analysis Suggests that Ongoing Affinity Maturation Drives the Emergence of Pemphigus Vulgaris Autoimmune Disease. Cell Rep 28:909-922.e6 (2019). PubMed: 31340153
    • Tung CP  et al. Discovering neutralizing antibodies targeting the stem epitope of H1N1 influenza hemagglutinin with synthetic phage-displayed antibody libraries. Sci Rep 5:15053 (2015). PubMed: 26456860
    • Isaacs AT  et al. Engineered resistance to Plasmodium falciparum development in transgenic Anopheles stephensi. PLoS Pathog 7:e1002017 (2011). WB . PubMed: 21533066
    • Mao X  et al. Autoimmunity to desmocollin 3 in pemphigus vulgaris. Am J Pathol 177:2724-30 (2010). WB . PubMed: 20952584
    • Xu L  et al. Combinatorial surrobody libraries. Proc Natl Acad Sci U S A 105:10756-61 (2008). WB, ELISA . PubMed: 18664586

    Customer reviews and Q&As

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    Question

    Are these 2 products (ab19400, ab19368) the same?

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    Answer

    Thank you for your enquiry. I can confirm that these 2 products are the same, just that ab19400 was HRP conjugated, and ab19368 was immobilized to agarose.

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