Recombinant HRP Anti-EAAT3 antibody [EPR6774(B)] (ab203479)
![Western blot - HRP Anti-EAAT3 antibody [EPR6774(B)] (ab203479)](https://www.abcam.com/ps/products/203/ab203479/Images/ab203479-248500-anti-eaat3-antibody-epr6774b-hrp-western-blot.jpg "Western blot - HRP Anti-EAAT3 antibody [EPR6774(B)] (ab203479)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- HRP Rabbit monoclonal [EPR6774(B)] to EAAT3
- Suitable for: WB
- Reacts with: Human
- Conjugation: HRP
Overview
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Product name
HRP Anti-EAAT3 antibody [EPR6774(B)]
See all EAAT3 primary antibodies -
Description
HRP Rabbit monoclonal [EPR6774(B)] to EAAT3 -
Host species
Rabbit -
Conjugation
HRP -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat
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Immunogen
Synthetic peptide within Human EAAT3 aa 150-250. The exact sequence is proprietary.
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Positive control
- WB: Fetal brain, Human testis and Fetal muscle tissue lysates.
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. Store In the Dark. -
Dissociation constant (KD)
KD = 3.80 x 10 -12 M Learn more about KD -
Storage buffer
pH: 7.40
Preservative: 0.1% 10% Proclin 300 Solution
Constituents: 30% Glycerol, 1% BSA, PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR6774(B) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Related Products
Applications
Our Abpromise guarantee covers the use of ab203479 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/5000. Detects a band of approximately 70 kDa (predicted molecular weight: 57 kDa). |
Target
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Function
Transports L-glutamate and also L- and D-aspartate. Essential for terminating the postsynaptic action of glutamate by rapidly removing released glutamate from the synaptic cleft. Acts as a symport by cotransporting sodium. Negatively regulated by ARL6IP5. -
Tissue specificity
Expressed in all tissues tested including liver, muscle, testis, ovary, retinoblastoma cell line, neurons and brain (in which there was dense expression in substantia nigra, red nucleus, hippocampus and in cerebral cortical layers). -
Sequence similarities
Belongs to the sodium:dicarboxylate (SDF) symporter (TC 2.A.23) family. SLC1A1 subfamily. -
Post-translational
modificationsGlycosylated. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 6505 Human
- Entrez Gene: 20510 Mouse
- Entrez Gene: 25550 Rat
- Omim: 133550 Human
- SwissProt: P43005 Human
- SwissProt: P51906 Mouse
- SwissProt: P51907 Rat
- Unigene: 444915 Human
see all -
Alternative names
- EAA3_HUMAN antibody
- EAAC 1 antibody
- EAAC1 antibody
see all
Images
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Western blot - HRP Anti-EAAT3 antibody [EPR6774(B)] (ab203479)All lanes : HRP Anti-EAAT3 antibody [EPR6774(B)] (ab203479) at 1/5000 dilution
Lane 1 : Brain (Human) Tissue Lysate - fetal normal tissue
Lane 2 : Testis (Human) Tissue Lysate - adult normal tissue
Lane 3 : Skeletal Muscle (Human) Tissue Lysate - adult normal tissue
Lysates/proteins at 10 µg per lane.
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 57 kDa
Observed band size: 70 kDa why is the actual band size different from the predicted?
Exposure time: 8 secondsThis blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab203479 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.
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![HRP Anti-EAAT3 antibody [EPR6774(B)] (ab203479)](https://www.abcam.com/ps/products/203/ab203479/Images/ab203479-3-benefits-of-recombinant-antibodies.png)
HRP Anti-EAAT3 antibody [EPR6774(B)] (ab203479)
References (0)
ab203479 has not yet been referenced specifically in any publications.
