Recombinant HRP Anti-ERK2 antibody [E460] (ab205819)
![Western blot - HRP Anti-ERK2 antibody [E460] (ab205819)](https://www.abcam.com/ps/products/205/ab205819/Images/ab205819-261166-anti-erk2-antibody-e460-hrp-western-blot.jpg "Western blot - HRP Anti-ERK2 antibody [E460] (ab205819)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- HRP Rabbit monoclonal [E460] to ERK2
- Suitable for: WB
- Knockout validated
- Reacts with: Mouse, Rat, Human, Recombinant fragment
- Conjugation: HRP
Overview
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Product name
HRP Anti-ERK2 antibody [E460]
See all ERK2 primary antibodies -
Description
HRP Rabbit monoclonal [E460] to ERK2 -
Host species
Rabbit -
Conjugation
HRP -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human, Recombinant fragment -
Immunogen
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Positive control
- WB: HeLa, 46C, PC12 whole cell lysates and Human ERK2 recombinant protein lysate.
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General notes
Alternative versions available:
Anti-ERK2 antibody [E460] (ab32081) - Knockout validatedThis product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.1% 10% Proclin 300 Solution
Constituents: 30% Glycerol, 1% BSA, PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
E460 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab205819 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/5000. Detects a band of approximately 41 kDa (predicted molecular weight: 41 kDa). |
Target
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Function
Involved in both the initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors such as ELK1. Phosphorylates EIF4EBP1; required for initiation of translation. Phosphorylates microtubule-associated protein 2 (MAP2). Phosphorylates SPZ1 (By similarity). Phosphorylates heat shock factor protein 4 (HSF4) and ARHGEF2.
Acts as a transcriptional repressor. Binds to a [GC]AAA[GC] consensus sequence. Repress the expression of interferon gamma-induced genes. Seems to bind to the promoter of CCL5, DMP1, IFIH1, IFITM1, IRF7, IRF9, LAMP3, OAS1, OAS2, OAS3 and STAT1. Transcriptional activity is independent of kinase activity. -
Sequence similarities
Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.
Contains 1 protein kinase domain. -
Domain
The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases. -
Post-translational
modificationsDually phosphorylated on Thr-185 and Tyr-187, which activates the enzyme. Dephosphorylated by PTPRJ at Tyr-187. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 5594 Human
- Entrez Gene: 26413 Mouse
- Entrez Gene: 116590 Rat
- Omim: 176948 Human
- SwissProt: P28482 Human
- SwissProt: P63085 Mouse
- SwissProt: P63086 Rat
- Unigene: 431850 Human
see all -
Alternative names
- ERK 2 antibody
- ERK-2 antibody
- ERT1 antibody
see all
Images
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Western blot - HRP Anti-ERK2 antibody [E460] (ab205819)All lanes : HRP Anti-ERK2 antibody [E460] (ab205819) at 1/5000 dilution
Lane 1 :HeLa whole cell lysate (ab150035) at 10 µg
Lane 2 : 46C (Mouse neural progenitor, selected for Sox1 expression cell line) Whole Cell Lysate at 10 µg
Lane 3 : PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate at 10 µg
Lane 4 : Recombinant Human ERK1 protein (ab43623) (ab43623) at 0.1 µg
Lane 5 :Recombinant Human ERK2 protein (ab43625) at 0.1 µg
Lane 6 : Wild-type HAP1 cell lysate at 20 µg
Lane 7 : ERK2 knockout HAP1 cell lysate at 20 µg
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 41 kDa
Observed band size: 41 kDa
Exposure time: 12 minutesThis blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab205819 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.
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![HRP Anti-ERK2 antibody [E460] (ab205819)](https://www.abcam.com/ps/products/205/ab205819/Images/ab205819-3-benefits-of-recombinant-antibodies.png)
HRP Anti-ERK2 antibody [E460] (ab205819)
References (0)
ab205819 has not yet been referenced specifically in any publications.
