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  1. Link

    hrp-erk2-antibody-e460-ab205819.pdf

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Neuroscience Neurotransmission Intracellular Signaling Kinases
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Validated using a knockout cell lineRecombinantRabMAb

Recombinant HRP Anti-ERK2 antibody [E460] (ab205819)

  • Datasheet
  • SDS
  • Certificate of Compliance
Submit a review Submit a question References (1)

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Western blot - HRP Anti-ERK2 antibody [E460] (ab205819)
  • HRP Anti-ERK2 antibody [E460] (ab205819)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • HRP Rabbit monoclonal [E460] to ERK2
  • Suitable for: WB
  • Knockout validated
  • Reacts with: Mouse, Rat, Human
  • Conjugation: HRP

Conjugates logo Related conjugates and formulations

Alexa Fluor® 555 Alexa Fluor® 647 Carrier Free Unconjugated

You may also be interested in

Primary
Product image
Anti-ERK1 (phospho T202) + ERK2 (phospho T185) antibody [EPR19401] (ab201015)
Knockout
Product image
Human MAPK1 (ERK2) knockout HeLa cell line (ab265052)
Protein
Product image
Recombinant Human ERK2 protein (ab95379)

View more associated products

Overview

  • Product name

    HRP Anti-ERK2 antibody [E460]
    See all ERK2 primary antibodies
  • Description

    HRP Rabbit monoclonal [E460] to ERK2
  • Host species

    Rabbit
  • Conjugation

    HRP
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HeLa, 46C, PC12 whole cell lysates and Human ERK2 recombinant protein lysate.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark.
  • Storage buffer

    pH: 7.40
    Preservative: 0.1% Proclin 300 Solution
    Constituents: 30% Glycerol (glycerin, glycerine), 1% BSA, PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    E460
  • Isotype

    IgG
  • Research areas

    • Neuroscience
    • Neurotransmission
    • Intracellular Signaling
    • Kinases
    • Signal Transduction
    • Protein Phosphorylation
    • Ser / Thr Kinases
    • MAPK Pathway
    • Stem Cells
    • Signaling Pathways
    • TGF beta
    • Cytoplasmic
    • Neuroscience
    • Development
    • Neuroscience
    • Processes

Associated products

  • Alternative Versions

    • Alexa Fluor® 647 Anti-ERK2 antibody [E460] (ab190581)
    • Alexa Fluor® 555 Anti-ERK2 antibody [E460] (ab213333)
    • Anti-ERK2 antibody [E460] - BSA and Azide free (ab214170)
    • Anti-ERK2 antibody [E460] (ab32081)
  • Isotype control

    • HRP Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab199507)
  • Positive Controls

    • A-431 whole cell lysate (ab7909)
  • Recombinant Protein

    • Recombinant Human ERK2 protein (ab95379)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab205819 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/5000. Detects a band of approximately 41 kDa (predicted molecular weight: 41 kDa).
Notes
WB
1/5000. Detects a band of approximately 41 kDa (predicted molecular weight: 41 kDa).

Target

  • Function

    Involved in both the initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors such as ELK1. Phosphorylates EIF4EBP1; required for initiation of translation. Phosphorylates microtubule-associated protein 2 (MAP2). Phosphorylates SPZ1 (By similarity). Phosphorylates heat shock factor protein 4 (HSF4) and ARHGEF2.
    Acts as a transcriptional repressor. Binds to a [GC]AAA[GC] consensus sequence. Repress the expression of interferon gamma-induced genes. Seems to bind to the promoter of CCL5, DMP1, IFIH1, IFITM1, IRF7, IRF9, LAMP3, OAS1, OAS2, OAS3 and STAT1. Transcriptional activity is independent of kinase activity.
  • Sequence similarities

    Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.
    Contains 1 protein kinase domain.
  • Domain

    The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.
  • Post-translational
    modifications

    Dually phosphorylated on Thr-185 and Tyr-187, which activates the enzyme. Dephosphorylated by PTPRJ at Tyr-187.
  • Cellular localization

    Nucleus.
  • Target information above from: UniProt accession P28482 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 5594 Human
    • Entrez Gene: 26413 Mouse
    • Entrez Gene: 116590 Rat
    • Omim: 176948 Human
    • SwissProt: P28482 Human
    • SwissProt: P63085 Mouse
    • SwissProt: P63086 Rat
    • Unigene: 431850 Human
    • Unigene: 196581 Mouse
    • Unigene: 34914 Rat
    see all
  • Alternative names

    • ERK 2 antibody
    • ERK-2 antibody
    • ERT1 antibody
    • Extracellular Signal Regulated Kinase 2 antibody
    • Extracellular signal-regulated kinase 2 antibody
    • MAP kinase 1 antibody
    • MAP kinase 2 antibody
    • MAP kinase isoform p42 antibody
    • MAPK 1 antibody
    • MAPK 2 antibody
    • Mapk1 antibody
    • MAPK2 antibody
    • Mitogen-activated protein kinase 1 antibody
    • Mitogen-activated protein kinase 2 antibody
    • MK01_HUMAN antibody
    • P38 antibody
    • P40 antibody
    • P41 antibody
    • p42-MAPK antibody
    • P42MAPK antibody
    • PRKM1 antibody
    • PRKM2 antibody
    • protein kinase, mitogen-activated, 1 antibody
    • protein kinase, mitogen-activated, 2 antibody
    • protein tyrosine kinase ERK2 antibody
    see all

Images

  • Western blot - HRP Anti-ERK2 antibody [E460] (ab205819)
    Western blot - HRP Anti-ERK2 antibody [E460] (ab205819)
    All lanes : HRP Anti-ERK2 antibody [E460] (ab205819) at 1/5000 dilution

    Lane 1 : HeLa whole cell lysate (ab150035) at 10 µg
    Lane 2 : 46C (Mouse neural progenitor, selected for Sox1 expression cell line) Whole Cell Lysate at 10 µg
    Lane 3 : PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate at 10 µg
    Lane 4 : Recombinant Human ERK1 protein (ab43623) (ab43623) at 0.1 µg
    Lane 5 : Recombinant Human ERK2 protein (ab43625) at 0.1 µg
    Lane 6 : Wild-type HAP1 cell lysate at 20 µg
    Lane 7 : ERK2 knockout HAP1 cell lysate at 20 µg

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 41 kDa
    Observed band size: 41 kDa


    Exposure time: 12 minutes


    This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab205819 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.

  • HRP Anti-ERK2 antibody [E460] (ab205819)
    HRP Anti-ERK2 antibody [E460] (ab205819)

Protocols

  • Western blot protocols

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

References (1)

Publishing research using ab205819? Please let us know so that we can cite the reference in this datasheet.

ab205819 has been referenced in 1 publication.

  • Li Q  et al. MicroRNA-186-5p downregulation inhibits osteoarthritis development by targeting MAPK1. Mol Med Rep 23:N/A (2021). PubMed: 33537828

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