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  1. Link

    hrp-estrogen-receptor-alpha-antibody-e115-ab194152.pdf

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Signal Transduction Signaling Pathway Nuclear Signaling Nuclear Hormone Receptors Estrogen
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RecombinantRabMAb

Recombinant HRP Anti-Estrogen Receptor alpha antibody [E115] (ab194152)

  • Datasheet
  • SDS
  • Certificate of Compliance
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-Estrogen Receptor alpha antibody [E115] (ab194152)
  • HRP Anti-Estrogen Receptor alpha antibody [E115] (ab194152)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • HRP Rabbit monoclonal [E115] to Estrogen Receptor alpha
  • Suitable for: IHC-P
  • Reacts with: Human
  • Conjugation: HRP

You may also be interested in

Protein
Product image
Recombinant Human Estrogen Receptor alpha protein (ab82606)

View more associated products

Overview

  • Product name

    HRP Anti-Estrogen Receptor alpha antibody [E115]
    See all Estrogen Receptor alpha primary antibodies
  • Description

    HRP Rabbit monoclonal [E115] to Estrogen Receptor alpha
  • Host species

    Rabbit
  • Conjugation

    HRP
  • Specificity

    Expression levels of ER alpha protein vary with sample type.
  • Tested applications

    Suitable for: IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide within Human Estrogen Receptor alpha. The exact sequence is proprietary.

  • Positive control

    • IHC/P: Human breast ductal carcinoma tissue.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark.
  • Storage buffer

    pH: 7.40
    Preservative: 0.1% 10% Proclin 300 Solution
    Constituents: 30% Glycerol, 1% BSA, PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    E115
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Signaling Pathway
    • Nuclear Signaling
    • Nuclear Hormone Receptors
    • Estrogen
    • Neuroscience
    • Endocrine system
    • Gonadotrophic axis
    • Epigenetics and Nuclear Signaling
    • Nuclear Signaling Pathways
    • Nuclear Receptors
    • Estrogen
    • Cancer
    • Signal transduction
    • Nuclear signaling
    • Nuclear hormone receptors
    • Estrogen

Associated products

  • Alternative Versions

    • Anti-Estrogen Receptor alpha antibody [E115] - Low endotoxin, Azide free (ab167611)
    • Alexa Fluor® 488 Anti-Estrogen Receptor alpha antibody [E115] (ab194150)
    • PE Anti-Estrogen Receptor alpha antibody [E115] (ab209288)
    • Anti-Estrogen Receptor alpha antibody [E115] - ChIP Grade (ab32063)
  • Isotype control

    • HRP Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab199507)
  • Recombinant Protein

    • Recombinant Human Estrogen Receptor alpha protein (ab82606)

Applications

Our Abpromise guarantee covers the use of ab194152 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    Nuclear hormone receptor. The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Can activate the transcriptional activity of TFF1.
  • Sequence similarities

    Belongs to the nuclear hormone receptor family. NR3 subfamily.
    Contains 1 nuclear receptor DNA-binding domain.
  • Domain

    Composed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain.
  • Post-translational
    modifications

    Phosphorylated by cyclin A/CDK2. Phosphorylation probably enhances transcriptional activity.
    Glycosylated; contains N-acetylglucosamine, probably O-linked.
    Ubiquitinated. Deubiquitinated by OTUB1.
    Dimethylated by PRMT1 at Arg-260. The methylation may favor cytoplasmic localization.
    Palmitoylated (isoform 3). Not biotinylated (isoform 3).
  • Cellular localization

    Nucleus. Cytoplasm. Cell membrane. A minor fraction is associated with the inner membrane and Nucleus. Cytoplasm. Cell membrane. Associated with the inner membrane via palmitoylation.
  • Target information above from: UniProt accession P03372 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 2099 Human
    • Entrez Gene: 13982 Mouse
    • Entrez Gene: 24890 Rat
    • Omim: 133430 Human
    • SwissProt: P03372 Human
    • SwissProt: P19785 Mouse
    • SwissProt: P06211 Rat
    • Unigene: 208124 Human
    • Unigene: 463262 Mouse
    • Unigene: 9213 Mouse
    • Unigene: 10595 Rat
    see all
  • Alternative names

    • 7*/654 isoform antibody
    • 7*/819 2 isoform antibody
    • 7*/822 isoform antibody
    • 8*/901 isoform antibody
    • 8*/941 isoform antibody
    • DKFZp686N23123 antibody
    • ER alpha antibody
    • ER antibody
    • ER-alpha antibody
    • Era antibody
    • ESR antibody
    • ESR1 antibody
    • ESR1_HUMAN antibody
    • ESRA antibody
    • Estradiol receptor antibody
    • Estrogen nuclear receptor alpha antibody
    • Estrogen receptor 1 antibody
    • Estrogen receptor alpha 3*,4,5,6,7*/822 isoform antibody
    • Estrogen receptor alpha antibody
    • Estrogen receptor alpha delta 3*,4,5,6,7*,8*/941 isoform antibody
    • Estrogen receptor alpha delta 3*,4,5,6,7*/819 2 isoform antibody
    • Estrogen receptor alpha delta 4 +49 isoform antibody
    • Estrogen receptor alpha delta 4*,5,6,7*/654 isoform antibody
    • Estrogen receptor alpha delta 4*,5,6,7,8*/901 isoform antibody
    • Estrogen receptor alpha E1 E2 1 2 antibody
    • Estrogen receptor alpha E1 N2 E2 1 2 antibody
    • Estrogen receptor antibody
    • ESTRR antibody
    • NR3A1 antibody
    • Nuclear receptor subfamily 3 group A member 1 antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-Estrogen Receptor alpha antibody [E115] (ab194152)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-Estrogen Receptor alpha antibody [E115] (ab194152)

    IHC image of Estrogen Receptor alpha staining in a section of formalin-fixed paraffin-embedded human breast ductal carcinoma*, performed on a Leica BOND. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab194152 at 1/100 dilution, for 15 mins at room temperature. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset negative control image is taken from an identical assay without primary antibody.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

  • HRP Anti-Estrogen Receptor alpha antibody [E115] (ab194152)
    HRP Anti-Estrogen Receptor alpha antibody [E115] (ab194152)

Protocols

  • Immunohistochemistry protocols

Click here to view the general protocols

Datasheets and documents

    • Datasheet
    • SDS

    Certificate of Compliance

    To download a Certificate of Compliance, please enter your Lot number below:

  • References (0)

    Publishing research using ab194152? Please let us know so that we can cite the reference in this datasheet.

    ab194152 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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